اثر افزودن چای سبز و روغن ماهی به جیره جوجه‌های گوشتی بر عملکرد و پاسخ ایمنی هومورال علیه نیوکاسل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد گروه علوم دامی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد ساوه

2 استادیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

3 استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد ساوه

چکیده

به‌منظور بررسی اثر چای سبز و روغن ماهی بر عملکرد و عیار پادتن علیه بیماری نیوکاسل، 405 قطعه جوجه گوشتی یکروزه در قالب طرح کاملاً تصادفی با استفاده از آزمون فاکتوریل 3×3 مورد بررسی قرار گرفتند. جوجه‌ها به 9 گروه و هر گروه به 3 دستۀ 15 قطعه‌ای تقسیم شدند. جوجه‌ها با 9 جیره حاوی مقادیر صفر (T0)، 75/0 (T0.75) یا 5/1 (T1.5) درصد پودر خشک چای سبز و مقادیر صفر (F0)، 75/0 (F0.75) یا 5/1 (F1.5) درصد روغن ماهی تغذیه شدند. واکسن نیوکاسل در روز 8 و 17 استفاده شد. جهت تعیین عیار پادتن علیه ویروس نیوکاسل به‌وسیله آزمایش ممانعت از هموآگلوتیناسیون (HI) در روزهای 25، 32 و 39، از ورید بال 5 جوجه در هر تکرار نمونه خون گرفته شد. نتایج نشان داد که در کل دوره ضریب تبدیل خوراک بین تیمار‌ها تفاوتی نداشت (05/0<P) و کمترین افزایش وزن روزانه (43/30) و بیشترین مصرف خوراک (25/73) در مقدار 5/1 درصد روغن ماهی و 5/1 درصد چای سبز مشاهد شد (05/0>P). در روز 39، بیشترین عیار پادتن (3) در سطح 5/1 درصد روغن ماهی مشاهده شد (05/0>P). در روزهای 32 و 39 عیار پادتن ضد ویروس نیوکاسل در سطح 5/1 درصد پودر خشک چای سبز (به‌ترتیب 09/2 و 18/2) کمتر از صفر درصد پودر خشک چای سبز (به‌ترتیب 64/2 و 84/2) بود (05/0>P). در نتیجه افزودن 5/1 درصد روغن ماهی و 5/1 درصد چای سبز به جیره جوجه‌های گوشتی به‌ترتیب سبب تحریک و تضعیف پاسخ‌های ایمنی هومورال علیه بیماری نیوکاسل می‌شود (05/0<P).

کلیدواژه‌ها


عنوان مقاله [English]

The effect of the addition of green tea and fish oil to diet of broiler on the performance and response of humoral immunity against Newcastle

نویسندگان [English]

  • S. Zare-Zade 1
  • M. Roostaei-Ali Mehr 2
  • A. R. Alizadeh 3
  • M. Mohammadi Gheysar 1
1 MSc student, Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Saveh, Iran
2 Assistant professor in the Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Iran
3 Assistant professor in the Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Saveh, Iran
چکیده [English]

In order to study the effect of green tea and fish oil on performance and antibody titer against Newcastle disease, 405 day-old broiler chickens were evaluated in a 3×3 factorial experiment using completely randomized design. Chicks were divided into 9 parts and each part was split into 3 groups. Each part was fed by diet containing 0% (T0), 0.75% (T0.75) or 1.5% (T1.5) dry powder of green tea and 0% (F0), 0.75% (F0.75) or 1.5% (F1.5) fish oil. Feed intake, gain weight and feed conversion ratio were estimated weekly. The vaccine of Newcastle was inoculated at 8 and 17d. To assay the antibody of Newcastle by HI test, blood samples were collected from five checks of each group from brachial vein at 25, 32 and 39 d. The results showed that there was no difference between treatments on the feed conversion ratio (P>0.05) and the lowest weight gain (30.43) and the highest feed intake (72.25) were observed by 1.5% fish oil and 1.5% green tea. At 39 d, the highest level of antibody (3) was obtained by 1.5 % fish oil (P<0.05). At 32 and 39d, the levels of antibody against Newcastle was lower in 1.5% green tea (2.09 and 2.18, respectively) than 0% green tea (2.64 and 2.84, respectively; P<0.05). Therefore, the addition of 1.5% fish oil and 1.5% green tea to diet of broiler chickens increased and decreased the humoral immunity responses against Newcastle disease, respectively.

کلیدواژه‌ها [English]

  • Feed conversion ratio
  • Fish Oil
  • Green tea
  • Newcastle
  • Immunity
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