Effect of different levels of silver nanoparticles coated with zeolite on performance, function of superoxide dismutase and glutathione peroxidase, carcass characteristics and internal organs weight of broiler chickens
M.
Esmaili
Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran.
author
S. R.
Hashemi
Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran.
author
Y.
Jafari Ahangari
Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran.
author
یوسف
جعفری آهنگری
گرگان - دانشگاه علوم کشاورزی و منابع طبیعی گرگان – دانشکده علوم دامی، گرگان، گلستان، ایران.
author
S.
Hassani
Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran.
author
A.
Shabani
Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran.
author
text
article
2017
per
The aim of this study was to evaluate the effect of different levels of silver nanoparticles coated with zeolite on performance, function of superoxide dismutase and glutathione peroxidase and level of malondialdehyde of broiler chickens. Three handered seventy five 1-day old broiler chicks divided into 5 treatments with 5 replicates (15 chicks per each) and were fed with basal diet, and basal diet supplemented with 1% zeolite and basal diet supplemented with 1% zeolite coated with 0.25%, 0.5% and 0.75% nanosilver. On days 21 and 42 two birds from each replicate were randomly selected and blood sample from their wing vein were collected to determine the malondialdehyde concentration, glutathione peroxidase and superoxide dismutase function in the serum, and then birds was slaughtered and carcass weight and relative weight percentage of thigh, breast and abdominal fat were measured. The result showed that feed conversion ratio of treatments coated with 0.25 (1.78), 0.5 (1/48) and 0.75 (1.92) percent of silver nanoparticles were not different from other treatments (P<0.05). Addition of zeolite coated with 0.5 percent of nanosilver increased function of superoxide dismutase on 21 (585 U/gHb) and 42(472.20 U/gHb) days and increase of breast muscle weight gain in 21 (50.11%) and 42 (39.19%) days (P>0.05). In conclusion, silver nanoparticles coated with zeolite at levels of 0.5% as feed additive in broiler diets improved oxidative enzymes concentrations and breast muscle relative weight percentage
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
1
11
https://ar.guilan.ac.ir/article_2102_822d8f25397acb34c24ae1af021a73e2.pdf
Identification of quantitative trait loci affecting average daily gain and Kleiber ratio on chromosome 5 in an F2 population of Japanese quail
M.
Iranmanesh
Graduated M.Sc., Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
author
A.
Esmailizadeh
Professor, Shahid Bahonar University of Kerman, Kerman
author
M. R.
Mohammad abadi
Professor, Shahid Bahonar University of Kerman, Kerman
author
S.
Sohrabi
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman
author
text
article
2017
per
This research carried out to identify effective quantitative trait loci in growth rate and Keliber ratio on chromosome 5 of Japanese quail by microsatellite markers. A three-generation resource population was developed by using two distinct Japanese quail strains, wild and white, to map quantitative trait loci underlying growth rate and Kleiber ratio. Eight pairs of white (S) and wild (W) birds were crossed reciprocally and 34 F1 birds were produced. The F1 birds were intercrossed to generate 422 F2 offspring. All of the animals from three generations (472 birds) were genotyped for three microsatellite markers on chromosome 5. Phenotypic data including average daily again and Kleiber ratio were collected on F2 birds. QTL analysis was performed using least squares regression interval mapping method fitting five various statistical models. Significant additive QTL were identified for average daily gain from hatch to one week of age and Kleiber ratio between 3 to 4 weeks of age. Dominance and imprinting QTL effects were not significant. The F2 phenotypic variance explained by the detected additive QTL effects ranged from 1.1 to 3.6 for different traits Significant QTLs identified by doing various statically types for average daily gain and keliber ratio with various effect.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
12
22
https://ar.guilan.ac.ir/article_2062_10da2cd93778d10cbef0512db501014b.pdf
Effect of different levels of garlic (Allium sativum) aqueous extract in drinking water on performance, blood lipids and intestinal microflora of Japanese quail
S.
Mahdizadeh
Graduated MSc student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
author
M.
Mohammadi
Associate Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
author
M.
Mohiti-Asli
Assistant Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
author
text
article
2017
per
The effects of garlic (Allium sativum) aqueous extract in drinking water were studied on performance, blood lipids and intestinal microflora using 280 Japanese quails in a completely randomized design with 5 treatments, 4 replicates and 14 birds per replicate. The treatments included 0, 0.5, 1, 1.5 and 2 mL garlic aqueous extract per litre of drinking water, respectively. On day 42, two birds per replicate were randomly selected and after sampling from ileum for determining the microflora populations, their carcasses were decomposed. Blood samples were collected on day 42 (one male and one female) and after separating the serum, triglycerides, total cholesterol, HDL and LDL were measured. The results indicated that consumption of garlic aqueous extract in drinking water had no significant effect on feed intake, daily gain, feed conversion ratio, weight of carcass, internal organs (P>0.05). Treatments had no significant effect on relative length of the small intestine segments including duodenum, jejunum and ileum, and serum lipids (P>0.05). The different levels of garlic extract had no effect on Lactodacillus and Coliform populations of ileum segment. Number of E.coli colony (CFU/g)in quails that received 2 mL extract (5.83) was lower (P<0.05) than the control group (5.95). It is concluded that the applied levels of garlic aqueous extract had no effect on the studied parameters in Japanese quail, however 2 mL extract reduced E.coli populations.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
23
32
https://ar.guilan.ac.ir/article_2055_8c667c58597667ed8b06d6af14641d82.pdf
Effect of in ovo (embryonic) inoculation of Arginine on pulmonary artery, right ventricular and lugs morphological changes in induced ascites broiler chickens
M.
Haghighat
Assistant Professor Animal Science Department, Faculty of Agriculture, Islamic Azad University, Golpayegan Branch
author
A. A.
Saki
Professor, Animal Science Department, Faculty of Agriculture, Bu-Ali Sina University
author
H. R.
Khodaee
Assistant Professor Animal Science Department, Faculty of Agriculture, Islamic Azad University, Golpayegan Branch
author
text
article
2017
per
In order to evaluate the influence of yolk in ovo (embryonic) inoculation of arginine in fifth day of incubation on pulmonary artery, right ventricular and lungs morphological changes in induced ascites broiler chickens, two separate experiments were designed. In first experiment, 560 fertile eggs divided in 7 groups and 4 subgroups and levels of 0, 20, 40, 60, 80 and 100 mg arginine per mL normal saline injected on yolk in fifth day of incubation and a group had no injection. In 20th day of incubation, nitric oxide (NO) and endothelin 1 (ET1) in the pulmonary artery in 2 fetuses per subgroup was measured. The 40 mg arginine per mL normal saline was the most appropriate level of arginine injection (lowest ET1 was 89.93 ng/100 mg tissue and highest NO was 13.35 µmol/100mg tissue). In the second experiment, 300 fertile eggs divided in 2 groups. In first group 40 mg of arginine per mL normal saline injected to 150 fertile eggs. The chickes were separated by sex after hatch. From day 21 to day 48 the rearing temperature reduced to 14-16 ℃ and ascite was induced. In ovo injection of arginine reduced ascites mortality (17.6 vs 48.8%), but the main effect of sex was insignificant (P>0.05). Main effect of arginine injection increased inner pulmonary artery area (3.47mm2 vs 2.67mm2) In treatments without arginine injection compared with those having argenine injection rather than arginine injection some gaps was observed in the lung cell layers. In ovo injection of arginine lowered the mortality rate due to ascites through increasing the pulmonary artery diameter and preserving the natural layers in the lungs.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
33
44
https://ar.guilan.ac.ir/article_2106_fb282dcc1c04c5cfad2172555101b58b.pdf
The Effect of Different Levels of Alcoholic Extract of White Mulberry Leaves (Morus alba L.) on Immune Responses and Lipid Peroxidation of Serum and Muscle of Broiler
M.
Seidasli
Graduate Master of Animal Physiology, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
author
M.
Roostaei-Ali Mehr
Associate Prof., Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
author
text
article
2017
per
This experiment was conducted to determine the effect of hydro-alcoholic extract of mulberry leaves on immune responses and lipid peroxidation by using 200 broiler chicks. Chickens were assigned to five groups and each group was divided into four categories with 10 birds. Diets were supplemented with 0, 2, 4, 6 and 8 % hydro-alcoholic extract of mulberry leaves in two periods of three-days (14-17 and 21-24 days). To evaluate cellular immune responses were evaluated by intra-dermal injection of at day16. Sheep red blood cells (SRBC) 25% (0.1 ml) was injected intramuscularly at days 16 and 23 and anti-SRBC IgM and IgG antibody titer were determined by hemagglutination test at days 19 and 26. Concentration of malondialdehyde was measured in serum (days 29 and 42) and muscle of raw and cooked thigh (day 42) after slaughtering birds and storage of thighs at -20 ℃ for six mouth. Results showed that, titer IgG was higher in 8 % blackberry leaf extract (5.7) than control (4.1) on day 26 (P<0.05). Concentration of malodialdehyde (nM/mL) in serum was lower in 8 % berry leaf extract (7.6) than control (7.8) on day 42 (P<0.05). Concentrations of malodialdehyde (nM/g) in raw meat were lower in treatments 8 (3), 6 (3.1) and 4 % (3.5) white mulberry leaf extract than control (4.7; P<0.05). Therefore, supplementation of diet with white mulberry leaf extract for short period of time improve humoral immune responses and quality of carcasses in broiler chicken.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
45
57
https://ar.guilan.ac.ir/article_2108_13410010a4b2c7cc8d0cf91010659978.pdf
Identification of Myostatin gene polymorphism by PCR-RFLP and its association with some morphological traits in some horse breeds
A.
Noshary
Assistant Professor, Animal Science Group, Agriculture Faculty, Islamic Azad University, Karaj Branch, Karaj, IRAN
author
A.
Lavvaf
Associate Professor, Animal Science Group, Agriculture Faculty, Islamic Azad University, Karaj Branch, Karaj, IRAN
author
text
article
2017
per
The morphological traits are polygenic and are affected by environment and polygenes, at the sametime. In this study, the Myostatin gene polymorphism in promoter region (MSTN) and its association with some morphological traits were studied in 120 random selected horses of Oldenburg, Turkmen, Thoroughbred and Caspian pony. After extraction of genomic DNA, PCR was done for 204 bp region of MSTN. The PCR-RFLP method according by SscpI was done for sample genotyping. The results showed that there are two TT and CT genotypes and T and C alleles for MSTN promoter locus in research samples. The MSTN genotypes had no significant effect on none of the chest width, height, hip and neck length traits across all studied breeds. The neck circuit for Thoroughbred and Caspian pony were 114.26 and 87.59 cm in TT genotype and 109.87 and 81.38 cm in CT genotype, respectively and differences were significant. In Oldenburg and Turkmen the TT genotype showed better performances for neck circuit however differences were no significant. In conclusion, there was polymorphism in the studied promoter region in all studied breeds and the MSTN polymorphism, as a candidate gene, can be used to identify the gentic potential differences in horse breeds.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
58
69
https://ar.guilan.ac.ir/article_2107_578e783ae2c0071be2f54912c9cc67c1.pdf
Identification of single nucleotide polymorphisms of PPP2CA gene and its effect on carcass, growth and fat storage traits in Lori Bakhtiari and Zel sheep
M.
Zare
M.Sc. gratuated of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran .
author
M.
Sadeghi
Associate professor of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
author
M.
Moradi-Shahrbabak
Professor of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
author
text
article
2017
per
The purpose of this study was to determine the polymorphism of PPP2CA gene in chromosome 5 of sheep and its effect on carcass, growth and fat storage traits in Lori-Bakhtiari and Zel sheep. Blood samples were collected from 140 Zel sheep (breeding or slaughtering), and 165 Lori-Bakhtiari sheep, and their DNA was extracted. Then, the fragment of 243 bp of exon 1 of PPP2CA gene was amplified of and its various patterns were sequenced. Two different banding patterns were observed and the results of sequencing showed existence of two mutations. Within the group of breeding Zel and Lori-Bakhtiari, the effect of T/T and Del/Del genotypes on weaning weight (27.79±1.46 vs 30.67 ± 1.57) and cholesterol (60.76±0.94 vs 54.26±1.65) was significant (P<0.05). In Lori-Bakhtiari, Del/Del genotype resulted in heavier fat tail (5.20±0.21kg) than T/T genotype (5.20±0.21kg vs 3.28±0.12kg) (P<0.05). In slaughtering Zel, the effect of genotypes on carcass fat percentage and triglyceride was significant, so that the T/T genotype had more carcass fat percentage (P<0.05) and Del/Del genotype had higher triglyceride (P<0.01). On the basis of the results, it is expected that this gene is affective on fat traits and should be considered in breeding programs.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
70
78
https://ar.guilan.ac.ir/article_2177_c051538aefe2fc288db966789fe8fc07.pdf
Determianation of chemical composition, gas production and in vitro fermentation parameters of tomato pulp and pistachio hull treated by Pleurotus sajor cajo
A.
Salehi
MS.c graduated, Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
author
D.
Alipour
Associate Professor, department of animal science, faculty of agriculture, Bu-Ali Sina University, Hamedan, Iran.
author
S.
Mirzaei
Assistant Professor, department of plant protection, faculty of agriculture, Bu-Ali Sina University, Hamedan, Iran.
author
Kh.
Zaboli
Assistant Professor, department of animal science, faculty of agriculture, Bu-Ali Sina University, Hamedan, Iran.
author
text
article
2017
per
This study was carried out to assess the nutritional value of tomato pulp (TP) and pistachio hull (PH) treated by pleurotus sajor cajo fungi. The treatments for TP were control (not incubated), positive control (incubated without inoculation), treated with mycelium and treated with spawn, and for PH were control (not incubated), positive control (incubated without inoculation) and treated with spawn. Twenty five grams per each two above-mentioned samples and three replicates of each treatments were poured into the glass flask and after sterilizing were kept inside an incubator at 25 °C for three weeks. Data were analyzed based on a completely randomized design. Dry matter, ash and crude protein concentrations from TP were 95.27, 2.40 and 12.81 percent and from PH were 94.63, 9.33 and 7.81 percent, respectively. Processing with fungi led to increase these components in both of two samples (P<0.05). Phenolic compounds of PH decreased due to treatment with fungi (P<0.05). In TP treated, comparing to control, gas production after 24 hours incubation, apparent in vitro dry matter digestibility and true in vitro organic matter digestibility reduced (P<0.05). Wheras, no changes were observed in PH, due to processing. In TP treated with spawn, compared with control group, the potential of gas production decreased (P<0.05). Overally, the results of this study showed that the nutritional value of tomato pulp and pistachio hull were not increased desirably by pleurotus sajor cajo treatment. This study was carried out to assess the nutritional value of tomato pulp (TP) and pistachio hull (PH) treated by pleurotussajorcajo fungi. The treatments for TP were control (not incubated), positive control (incubated without inoculation), treated with mycelium and treated with spawn, and for PH were control (not incubated), positive control (incubated without inoculation) and treated with spawn. Twenty five grams per each two above-mentioned samples and three replicates of each treatments were poured into the glass flask and after sterilizing were kept inside an incubator at 25 °C for three weeks. Data were analyzed based on a completely randomized design. Dry matter, ash and crude protein concentrations from TP were 95.27, 2.40 and 12.81 percent and from PH were 94.63, 9.33 and 7.81 percent, respectively. Processing with fungi led to increase these components in both of two samples (P<0.05). Phenolic compounds of PH decreased due to treatment with fungi (P<0.05). In TP treated, comparing to control, gas production after 24 hours incubation, apparent in vitro dry matter digestibility and true in vitro organic matter digestibility reduced (P<0.05). Wheras, no changes were observed in PH, due to processing. In TP treated with spawn, compared with control group, the potential of gas production decreased (P<0.05). Overally, the results of this study showed that the nutritional value of tomato pulp and pistachio hull were not increased desirably by pleurotussajorcajo treatment.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
79
91
https://ar.guilan.ac.ir/article_2176_df71d13074caa2204b38143f16dc3f6f.pdf
Effect of feeding different fat sources on milk production and composition and blood metabolites in Hostein dairy cow during transition period
Y.
Rajabi
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
author
Y.
Chashnidel
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
author
E.
Dirandeh
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
author
text
article
2017
per
The aim of this study was to investigate the effects of different sources of fat on milk production and composition and blood metabolites during the transition period in the Holstein dairy cow. Sixty multiparous Holstein cows were chosen randomly according to expected parturition. Cows were assigned to 3 groups from 21 days prior to calving until 21 days postpartum. Groups included: 1. control diet with protected palm oil (n = 20), 2. extruded linseed (n =20) and 3. soybean whole roast (n= 20). The diets were similar in dry matter and crude protein content but were different in fat sources. Milk production and composition were measured weekly. Blood samples collected weekly to measure glucose, beta-hydroxybutyrate (BHBA), non-esterified fatty acids (NEFA), high density lipoprotein (HDL), triglycerides and total cholesterol. Results showed milk yield and DMI were not affected by experimental diets (P > 0.05). Milk compositions were similar (P > 0.05) among diets, except milk fat percentage and milk fat yield which was affected by experimental diets (P < 0.05) and were less in cows fed control and soybean whole roast diets. Triglycerides, NEFA and BHBA concentrations were significantly lower in cows fed extruded linseed compared to control and soybean whole roast groups (P < 0.05). The overall results showed that feeding diets containing soybean and extruded flaxseed can improve health status by decreasing milk fat percentage, NEFA and BHBA in dairy cows at the transition period.
Animal Production Research
University of Guilan
2252-0872
5
v.
4
no.
2017
92
100
https://ar.guilan.ac.ir/article_2188_c2467f249b0f0d390f86173d039753a6.pdf