University of Guilan Animal Production Research 2252-0872 5 1 2016 05 21 Effects of Lemon Balm (Melissa officinalis) essential oil on performance, mortality and some blood parameters related to ascites in broiler chickens Effects of Lemon Balm (Melissa officinalis) essential oil on performance, mortality and some blood parameters related to ascites in broiler chickens 27 37 1723 FA M. Fathi Assistant Professor of Animal Science, Department of Agriculture, Payam Noor University, Tehran, Iran M. Haydari Scientific Member of Animal Science, Department of Agriculture, Payam Noor University, Tehran, Iran Journal Article 2016 06 26 This experiment was conducted to evaluate the effect of different levels of Essential Oil on Growth, mortality and Ascites Parameters in Broiler chickens. Research was performed in a completely randomize design with 4 treatments, 5 replicate and 30 broilers in each replicate for 49 days. The treatments consist of the control  (0), 5, 10 and 20 ml of Lemon Balm essential oil in drinking water. At day 49, four chicks from each replicate were randomly slaughtered and ascetic index calculated Total red blood cell, Wight blood cell, hemoglobin, hematocrit, glucose, protein, cholesterol, triglycerides, high density lipoprotein (HDL), activity of Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Cratine Kinase  (CK), Glutathione peroxidase (GPX), superoxide dismutase (SOD) total antioxidant status (TAS) and malondialdehyde (MDA) content of plasma were determined at day 49. Results showed that, 5 ml of essential oil had no significant effect on growth performance but, reduced ascetic index (0.24), mortality due to ascites (%10).It is also, 5 ml of essential oil significantly, reduced MDA (2.02 n mol/ml), cholesterol (92.56 mg/dl), AST, ALP and CK activity in plasma (254, 675 and 2495 U/L).  Moreover, level of 5 ml of essential oil increased GPx activity (278 u/g Hg)  and TAS (1.97 n mol/ml) in plasma. In concluded that, 5 ml of essential oil of lemon balm can reduce mortality due to Ascites in broilers. This experiment was conducted to evaluate the effect of different levels of Essential Oil on Growth, mortality and Ascites Parameters in Broiler chickens. Research was performed in a completely randomize design with 4 treatments, 5 replicate and 30 broilers in each replicate for 49 days. The treatments consist of the control  (0), 5, 10 and 20 ml of Lemon Balm essential oil in drinking water. At day 49, four chicks from each replicate were randomly slaughtered and ascetic index calculated Total red blood cell, Wight blood cell, hemoglobin, hematocrit, glucose, protein, cholesterol, triglycerides, high density lipoprotein (HDL), activity of Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Cratine Kinase  (CK), Glutathione peroxidase (GPX), superoxide dismutase (SOD) total antioxidant status (TAS) and malondialdehyde (MDA) content of plasma were determined at day 49. Results showed that, 5 ml of essential oil had no significant effect on growth performance but, reduced ascetic index (0.24), mortality due to ascites (%10).It is also, 5 ml of essential oil significantly, reduced MDA (2.02 n mol/ml), cholesterol (92.56 mg/dl), AST, ALP and CK activity in plasma (254, 675 and 2495 U/L).  Moreover, level of 5 ml of essential oil increased GPx activity (278 u/g Hg)  and TAS (1.97 n mol/ml) in plasma. In concluded that, 5 ml of essential oil of lemon balm can reduce mortality due to Ascites in broilers. https://ar.guilan.ac.ir/article_1723_10ef881289dceb770fe2941543b5170c.pdf