University of Guilan Animal Production Research 2252-0872 5 4 2017 02 19 The Effect of Different Levels of Alcoholic Extract of White Mulberry Leaves (Morus alba L.) on Immune Responses and Lipid Peroxidation of Serum and Muscle of Broiler The Effect of Different Levels of Alcoholic Extract of White Mulberry Leaves (Morus alba L.) on Immune Responses and Lipid Peroxidation of Serum and Muscle of Broiler 45 57 2108 FA M. Seidasli Graduate Master of Animal Physiology, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran M. Roostaei-Ali Mehr Associate Prof., Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran 0000-0001-7578-467X Journal Article 2016 08 30 This experiment was conducted to determine the effect of hydro-alcoholic extract of mulberry leaves on immune responses and lipid peroxidation by using 200 broiler chicks. Chickens were assigned to five groups and each group was divided into four categories with 10 birds.  Diets were supplemented with 0, 2, 4, 6 and 8 % hydro-alcoholic extract of mulberry leaves in two periods of three-days  (14-17 and 21-24 days). To evaluate cellular immune responses were evaluated by intra-dermal injection of at day16. Sheep red blood cells (SRBC) 25% (0.1 ml) was injected intramuscularly at days 16 and 23 and anti-SRBC IgM and IgG antibody titer were determined by hemagglutination test at days 19 and 26. Concentration of malondialdehyde was measured in serum (days 29 and 42) and muscle of raw and cooked thigh (day 42) after slaughtering birds and storage of thighs at -20 ℃ for six mouth. Results showed that, titer IgG was higher in 8 % blackberry leaf extract (5.7) than control (4.1) on day 26 (P<0.05). Concentration of malodialdehyde (nM/mL) in serum was lower in 8 % berry leaf extract (7.6) than control (7.8) on day 42 (P<0.05). Concentrations of malodialdehyde (nM/g) in raw meat were lower in treatments 8 (3), 6 (3.1) and 4 % (3.5) white mulberry leaf extract than control (4.7; P<0.05). Therefore, supplementation of diet with white mulberry leaf extract for short period of time improve humoral immune responses and quality of carcasses in broiler chicken. This experiment was conducted to determine the effect of hydro-alcoholic extract of mulberry leaves on immune responses and lipid peroxidation by using 200 broiler chicks. Chickens were assigned to five groups and each group was divided into four categories with 10 birds.  Diets were supplemented with 0, 2, 4, 6 and 8 % hydro-alcoholic extract of mulberry leaves in two periods of three-days  (14-17 and 21-24 days). To evaluate cellular immune responses were evaluated by intra-dermal injection of at day16. Sheep red blood cells (SRBC) 25% (0.1 ml) was injected intramuscularly at days 16 and 23 and anti-SRBC IgM and IgG antibody titer were determined by hemagglutination test at days 19 and 26. Concentration of malondialdehyde was measured in serum (days 29 and 42) and muscle of raw and cooked thigh (day 42) after slaughtering birds and storage of thighs at -20 ℃ for six mouth. Results showed that, titer IgG was higher in 8 % blackberry leaf extract (5.7) than control (4.1) on day 26 (P<0.05). Concentration of malodialdehyde (nM/mL) in serum was lower in 8 % berry leaf extract (7.6) than control (7.8) on day 42 (P<0.05). Concentrations of malodialdehyde (nM/g) in raw meat were lower in treatments 8 (3), 6 (3.1) and 4 % (3.5) white mulberry leaf extract than control (4.7; P<0.05). Therefore, supplementation of diet with white mulberry leaf extract for short period of time improve humoral immune responses and quality of carcasses in broiler chicken. https://ar.guilan.ac.ir/article_2108_13410010a4b2c7cc8d0cf91010659978.pdf