University of Guilan Animal Production Research 2252-0872 4 1 2015 05 22 Genetic variation of camels in the North of Kerman province using microsatellite markers Genetic variation of camels in the North of Kerman province using microsatellite markers 35 45 211 FA M. Ghasemi Meymandi MSc Student of Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman. M. R. Mohammadabadi Associate Professor of Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman A. K. Esmailizadeh Professor of Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman Journal Article 2015 06 16 This study investigated genetic variation within indigenous Camelus dromedarius in north of Kerman province using five microsatellite markers (VOLP03, VOLP08, YWLL08, YWLL38 and CVR01). The blood samples were collected from 81 camels of Shahr Babak, Rafsanjan and Ravar (5 populations). Phenol - chloroform extraction method was used to isolate the DNA. The highest and the lowest allele number and effective alleles are shown in YWLL08 (14 and 10.54) and CVR01 (5 and 4.35), respectively. The expected heterozygosity varied from 0.9108 (YWLL08) to 0.7754 (CVR01). The Hardy-Weinberg equilibrium (HWE) test showed that all loci deviated from HWE (P<0.05). Polymorphism information content ranged from 0.7801 to 0.9165. Fixation index (FST) values for markers YWLL08, VOLP03, VOLP08, YWLL38 and CVR01 was obtained respectively 0.036, 0.089, 0.073, 0.046, 0.054 and 0.056 that indicates the distinction is too much between the populations. The results of the current study indicated that the Camelus dromedarius in the North of Kerman province populations have a relativity high genetic variation, which makes them compatible to drastic environmental changes existing in Kerman province. Therefore, it is essential to maintain this genetic diversity and this valuable animal. This study investigated genetic variation within indigenous Camelus dromedarius in north of Kerman province using five microsatellite markers (VOLP03, VOLP08, YWLL08, YWLL38 and CVR01). The blood samples were collected from 81 camels of Shahr Babak, Rafsanjan and Ravar (5 populations). Phenol - chloroform extraction method was used to isolate the DNA. The highest and the lowest allele number and effective alleles are shown in YWLL08 (14 and 10.54) and CVR01 (5 and 4.35), respectively. The expected heterozygosity varied from 0.9108 (YWLL08) to 0.7754 (CVR01). The Hardy-Weinberg equilibrium (HWE) test showed that all loci deviated from HWE (P<0.05). Polymorphism information content ranged from 0.7801 to 0.9165. Fixation index (FST) values for markers YWLL08, VOLP03, VOLP08, YWLL38 and CVR01 was obtained respectively 0.036, 0.089, 0.073, 0.046, 0.054 and 0.056 that indicates the distinction is too much between the populations. The results of the current study indicated that the Camelus dromedarius in the North of Kerman province populations have a relativity high genetic variation, which makes them compatible to drastic environmental changes existing in Kerman province. Therefore, it is essential to maintain this genetic diversity and this valuable animal.

https://ar.guilan.ac.ir/article_211_b9f1c449165870351f1a04aad6f27446.pdf