University of Guilan Animal Production Research 2252-0872 6 1 2017 06 22 The effect of long-term dietary cadmium chloride on cadmium concentration in tissues, urine and feces and concentration of iron, zinc and copper in tissues in Holstein male calves The effect of long-term dietary cadmium chloride on cadmium concentration in tissues, urine and feces and concentration of iron, zinc and copper in tissues in Holstein male calves 1 11 2172 10.22124/ar.2017.2172 FA M. A. Khorashadi Zadeh PhD Student, Department of Animal Sciences, Faculty of Agricultural Science, Ferdowsi University of Mashhad, Iran R. Valizadeh Professor, Department of Animal Sciences, Faculty of Agricultural Science, Ferdowsi University of Mashhad, Iran A. A. Naserian Professor, Department of Animal Sciences, Faculty of Agricultural Science, Ferdowsi University of Mashhad, Iran Journal Article 2016 10 23 The effects of long-term consumption of cadmium (Cd) on its concentration in liver, kidney, lunges and muscles tissues, blood, urine and feces and also iron, zinc and copper content in liver, kidney, bones and muscles tissues in Holstein male calves were studied in this study. Twenty Holstein male calves (with average body weight of 203 kg and age of 185 days) were allocated to four groups in a completely randomized design. All of the animals were fed a similar diet but differing in concentration of Cd (0, 2, 5 and 10 mg/kg of dry matter) for a period of 300 days. The blood, urine and feces of all experimental calves were sampled at the end of last 3 days of the experimental period. The calves were slaughtered at the end of experimental period and their liver, kidney, lunges, shinbone, thigh and heart muscles and spleen were also sampled. The results revealed that, Cd concentration in liver and kidney tissues urine, and feces samples were significantly (<em>P</em><0.05) higher than the control calves. With increasing Cd intake, the copper concentration in liver and bone and the zinc concentration in liver and kidney decreased significantly (<em>P</em><0.05). It was concluded that soil and water pollution with Cd could increase its concentration in animal body and products which is harmful for their consumers. The effects of long-term consumption of cadmium (Cd) on its concentration in liver, kidney, lunges and muscles tissues, blood, urine and feces and also iron, zinc and copper content in liver, kidney, bones and muscles tissues in Holstein male calves were studied in this study. Twenty Holstein male calves (with average body weight of 203 kg and age of 185 days) were allocated to four groups in a completely randomized design. All of the animals were fed a similar diet but differing in concentration of Cd (0, 2, 5 and 10 mg/kg of dry matter) for a period of 300 days. The blood, urine and feces of all experimental calves were sampled at the end of last 3 days of the experimental period. The calves were slaughtered at the end of experimental period and their liver, kidney, lunges, shinbone, thigh and heart muscles and spleen were also sampled. The results revealed that, Cd concentration in liver and kidney tissues urine, and feces samples were significantly (<em>P</em><0.05) higher than the control calves. With increasing Cd intake, the copper concentration in liver and bone and the zinc concentration in liver and kidney decreased significantly (<em>P</em><0.05). It was concluded that soil and water pollution with Cd could increase its concentration in animal body and products which is harmful for their consumers. https://ar.guilan.ac.ir/article_2172_07f4b060db28302cd1acde8a568075db.pdf