University of Guilan Animal Production Research 2252-0872 5 4 2017 02 19 Identification of single nucleotide polymorphisms of PPP2CA gene and its effect on carcass, growth and fat storage traits in Lori Bakhtiari and Zel sheep Identification of single nucleotide polymorphisms of PPP2CA gene and its effect on carcass, growth and fat storage traits in Lori Bakhtiari and Zel sheep 70 78 2177 FA M. Zare M.Sc. gratuated of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran . M. Sadeghi Associate professor of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. M. Moradi-Shahrbabak Professor of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Journal Article 2016 02 14 The purpose of this study was to determine the polymorphism of PPP2CA gene in chromosome 5 of sheep and its effect on carcass, growth and fat storage traits in Lori-Bakhtiari and Zel sheep. Blood samples were collected from 140 Zel sheep (breeding or slaughtering), and 165 Lori-Bakhtiari sheep, and their DNA was extracted. Then, the fragment of 243 bp of exon 1 of PPP2CA gene was amplified of and its various patterns were sequenced. Two different banding patterns were observed and the results of sequencing showed existence of two mutations. Within the group of breeding Zel and Lori-Bakhtiari, the effect of T/T and Del/Del genotypes on weaning weight (27.79±1.46 vs 30.67 ± 1.57) and cholesterol (60.76±0.94 vs 54.26±1.65) was significant (P<0.05). In Lori-Bakhtiari, Del/Del genotype resulted in heavier fat tail (5.20±0.21kg) than T/T genotype (5.20±0.21kg vs 3.28±0.12kg) (P<0.05). In slaughtering Zel, the effect of genotypes on carcass fat percentage and triglyceride was significant, so that the T/T genotype had more carcass fat percentage (<em>P</em><0.05) and Del/Del genotype had higher triglyceride (<em>P</em><0.01). On the basis of the results, it is expected that this gene is affective on fat traits and should be considered in breeding programs. The purpose of this study was to determine the polymorphism of PPP2CA gene in chromosome 5 of sheep and its effect on carcass, growth and fat storage traits in Lori-Bakhtiari and Zel sheep. Blood samples were collected from 140 Zel sheep (breeding or slaughtering), and 165 Lori-Bakhtiari sheep, and their DNA was extracted. Then, the fragment of 243 bp of exon 1 of PPP2CA gene was amplified of and its various patterns were sequenced. Two different banding patterns were observed and the results of sequencing showed existence of two mutations. Within the group of breeding Zel and Lori-Bakhtiari, the effect of T/T and Del/Del genotypes on weaning weight (27.79±1.46 vs 30.67 ± 1.57) and cholesterol (60.76±0.94 vs 54.26±1.65) was significant (P<0.05). In Lori-Bakhtiari, Del/Del genotype resulted in heavier fat tail (5.20±0.21kg) than T/T genotype (5.20±0.21kg vs 3.28±0.12kg) (P<0.05). In slaughtering Zel, the effect of genotypes on carcass fat percentage and triglyceride was significant, so that the T/T genotype had more carcass fat percentage (<em>P</em><0.05) and Del/Del genotype had higher triglyceride (<em>P</em><0.01). On the basis of the results, it is expected that this gene is affective on fat traits and should be considered in breeding programs. https://ar.guilan.ac.ir/article_2177_c051538aefe2fc288db966789fe8fc07.pdf