University of Guilan Animal Production Research 2252-0872 6 1 2017 06 27 The effect of In ovo injection of zinc-methionine and nano-zinc methionine on the Zn-T1 gene expression, alkaline phosphatase and maltase activity in broilers small intestine The effect of In ovo injection of zinc-methionine and nano-zinc methionine on the Zn-T1 gene expression, alkaline phosphatase and maltase activity in broilers small intestine 73 87 2222 10.22124/ar.2017.2222 FA K. Razani Ph.D student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran M. Mottaghitalab Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran S. H. Hossieni Moghaddam Assistant professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran Journal Article 2016 12 30 The aim of this study was to investigate the effect of intra amniotic injection of zinc-methionine and nano-zinc-methionine on intestinal zinc transporter (zn-t1)expression and alkaline phosphatase and maltase activity in the broiler jejunum. Four hundred fertile eggs were divided into four groups (treatment), each with four samples (replicate). The first three treatments were subject to intra amniotic injection of one milliliter of physiologic serum solution, physiologic serum solution containing 25% zinc- methionine and/or containing 25% nano-zinc- methionine, respectively, at the 17^th day of incubation. The fourth treatment (negative control) received no injection. The hatchability percentage was calculated and two birds from each replicate were killed then intestinal alkaline phosphatase and maltase activity and Zn-T1gene expression were assayed at 1, 3 and 7 days after hatch. The highest body weight was for Zinc-methionine (43.02± 0.06) and Nano-zinc-methionine (42.77±0.06) treatments (<em>P</em>˂0.01). Also at 1 and 7 days of post hatch the weight of small intestine in the Zinc-methionine (1.55±0.07, 9.7±0.048) and Nano-zinc-methionine (1.51±0.07, 9.5±0.048) treatments were higher than other treatments (<em>p</em>˂0.01). Ten nano-zinc-methionine treatment, at 1, 3 and 7 days after hatching, had the most activity of alkaline phosphatase (7.2 ± 0.04, 11.5± 0.08, 17.8 ± 0.1) and maltase (4.2±0.03, 5.5±0.02, 8.8±0.06) andmost Zn-T1 gene expression (3.67±0.005, 4.24±0.007, 3.17±0.009) (<em>P</em>˂0.01). In conclusion, <em>in Ovo</em> injection of Nano-zinc-methionine showed up-regulate Zn-T1 gene expression and lead to increase alkaline phosphatase and maltase activity of chick intestine during first week after hatch. <br /><em> </em> The aim of this study was to investigate the effect of intra amniotic injection of zinc-methionine and nano-zinc-methionine on intestinal zinc transporter (zn-t1)expression and alkaline phosphatase and maltase activity in the broiler jejunum. Four hundred fertile eggs were divided into four groups (treatment), each with four samples (replicate). The first three treatments were subject to intra amniotic injection of one milliliter of physiologic serum solution, physiologic serum solution containing 25% zinc- methionine and/or containing 25% nano-zinc- methionine, respectively, at the 17^th day of incubation. The fourth treatment (negative control) received no injection. The hatchability percentage was calculated and two birds from each replicate were killed then intestinal alkaline phosphatase and maltase activity and Zn-T1gene expression were assayed at 1, 3 and 7 days after hatch. The highest body weight was for Zinc-methionine (43.02± 0.06) and Nano-zinc-methionine (42.77±0.06) treatments (<em>P</em>˂0.01). Also at 1 and 7 days of post hatch the weight of small intestine in the Zinc-methionine (1.55±0.07, 9.7±0.048) and Nano-zinc-methionine (1.51±0.07, 9.5±0.048) treatments were higher than other treatments (<em>p</em>˂0.01). Ten nano-zinc-methionine treatment, at 1, 3 and 7 days after hatching, had the most activity of alkaline phosphatase (7.2 ± 0.04, 11.5± 0.08, 17.8 ± 0.1) and maltase (4.2±0.03, 5.5±0.02, 8.8±0.06) andmost Zn-T1 gene expression (3.67±0.005, 4.24±0.007, 3.17±0.009) (<em>P</em>˂0.01). In conclusion, <em>in Ovo</em> injection of Nano-zinc-methionine showed up-regulate Zn-T1 gene expression and lead to increase alkaline phosphatase and maltase activity of chick intestine during first week after hatch. <br /><em> </em> https://ar.guilan.ac.ir/article_2222_a5e5e9446c20b5c027f9eefe52945850.pdf