University of Guilan Animal Production Research 2252-0872 2 2 2013 08 23 Effect of heat stress on in vitro culture of ram Sertoli cell Effect of heat stress on in vitro culture of ram Sertoli cell 11 17 231 FA F. Salehinejhad M.Sc. Student, Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran A. Lavvaf Associate Professor, Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran A. Kadivar Assistant Professor, Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran H. Hassanpour Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran H. Nazari Ph.D Student, Reproductive biotechnology, Shahrekord University, Shahrekord, Iran Journal Article 2015 06 20 The present study was conducted to evaluate some oxidative stress in ram Sertoli cells under heat stress condition. The testis of 10 ram lambs was gathered from abattoir (during 5 times) and transferred to laboratory for isolation and culture of Sertoli cells. Isolated cells from lamb testes were divided into 3 plates and maintained in temperatures 32 ºC (control), 39 ºC (mild stress) and 42 ºC (severe stress) for 6 hours. The percentage of cell viability, the amount of lipid peroxidation (TBARS), the antioxidant capacity (FRAP) and superoxide dismutase activity were measured in all groups after 6 hours. The percentage of alive cells were reduced in group 42 ºC (78 ± 6.8) as compared to control (95 ± 9.3; P<0.05). Lopud neroxidation was higher in sever stress (0.65 ± 0.06) than control (0.46 ± 0.05; P<0.05). In conclusion, heat stress could be a detrimental factor for viability of Sertoli cells via increasing of lipid peroxidation. The present study was conducted to evaluate some oxidative stress in ram Sertoli cells under heat stress condition. The testis of 10 ram lambs was gathered from abattoir (during 5 times) and transferred to laboratory for isolation and culture of Sertoli cells. Isolated cells from lamb testes were divided into 3 plates and maintained in temperatures 32 ºC (control), 39 ºC (mild stress) and 42 ºC (severe stress) for 6 hours. The percentage of cell viability, the amount of lipid peroxidation (TBARS), the antioxidant capacity (FRAP) and superoxide dismutase activity were measured in all groups after 6 hours. The percentage of alive cells were reduced in group 42 ºC (78 ± 6.8) as compared to control (95 ± 9.3; P<0.05). Lopud neroxidation was higher in sever stress (0.65 ± 0.06) than control (0.46 ± 0.05; P<0.05). In conclusion, heat stress could be a detrimental factor for viability of Sertoli cells via increasing of lipid peroxidation. https://ar.guilan.ac.ir/article_231_f85fad072baf088348b4d1f41f00553e.pdf