University of Guilan Animal Production Research 2252-0872 7 4 2019 02 20 Immunohistochemical localization of Ghrelin in sex accessory glands of bull Immunohistochemical localization of Ghrelin in sex accessory glands of bull 79 88 3321 10.22124/ar.2019.10142.1302 FA M. Rahmi MSc. Graduated, Department of Animal Science, Agriculture Faculty, Sanandaj branch, Islaic Azad University, Kurdistan, Iran B. Shokrollahi Associate Professor, Department of Animal Science, Agriculture Faculty, Sanandaj branch, Islaic Azad University, Kurdistan, Iran A. A. Amiri Lecturer, Department of Basic Sciences, Veterinary Faculty, Sanandaj branch, Islaic Azad University, Kurdistan, Iran Journal Article 2018 04 15 Ghrelin is a 28 amino acid peptide hormone that is expressed in the stomach and a range of peripheral tissues. Expression and functional role of ghrelin in the sex accessory glands of mammals have not been studied yet (except for human). This study was aimed to determine the immunohistochemical position of ghrelin in prostate, seminal vesicles and ampulla tissues of Holstein bulls. Anti-ghrelin mouse monoclonal antibody was used as primary antibody and donkey polyclonal antibody Anti-IgG Horseradish Peroxidase (HRP) was used as secondary antibody. Samples of sex accessory glands tissues were collected from five Holstein bulls aged two years old, and preserved in 10% buffered formalin for the immunohistochemical test. Then, paraffin blocks and tissue sections were prepared. The immunohistochemical results were positive for the secretory cells of prostate, seminal vesicles and ampulla. But the intensity of staining in the prostate glands was very weak than to the other glands. According to the results of this research, the expression of ghrelin in some accessory glands of the bull may display its possible role in fertility parameters of sperm and overall fertilization process. Ghrelin is a 28 amino acid peptide hormone that is expressed in the stomach and a range of peripheral tissues. Expression and functional role of ghrelin in the sex accessory glands of mammals have not been studied yet (except for human). This study was aimed to determine the immunohistochemical position of ghrelin in prostate, seminal vesicles and ampulla tissues of Holstein bulls. Anti-ghrelin mouse monoclonal antibody was used as primary antibody and donkey polyclonal antibody Anti-IgG Horseradish Peroxidase (HRP) was used as secondary antibody. Samples of sex accessory glands tissues were collected from five Holstein bulls aged two years old, and preserved in 10% buffered formalin for the immunohistochemical test. Then, paraffin blocks and tissue sections were prepared. The immunohistochemical results were positive for the secretory cells of prostate, seminal vesicles and ampulla. But the intensity of staining in the prostate glands was very weak than to the other glands. According to the results of this research, the expression of ghrelin in some accessory glands of the bull may display its possible role in fertility parameters of sperm and overall fertilization process. https://ar.guilan.ac.ir/article_3321_c2d3c7ff33a7a2fe92250d335f446073.pdf