University of Guilan Animal Production Research 2252-0872 8 4 2020 02 20 Evaluation of genetic variations of the PRNP gene in Mehraban and Romanov sheep breeds Evaluation of genetic variations of the PRNP gene in Mehraban and Romanov sheep breeds 9 18 3536 10.22124/ar.2019.12995.1401 FA M. Hashemi MSc. Graduated in Animal Breeding and Genetics, Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran A. Ahmadi Assistant Professor, Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran R. Abdoli Ph.D Graduated in Animal Breeding and Genetics, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran 0000-0003-0792-1980 Journal Article 1970 01 01 This study was conducted to investigate the polymorphisms of the Prion protein gene (PRNp ) exon 3 in Mehraban and Romanov sheep. Blood samples were collected from 124 sheep (85 Mahraban and 39 Romanov). After DNA extraction, polymerase chain reactions (PCR) were performed to amplify a 173 bp fragment of the PRNp gene exon 3 using a specific pair of primers. Polymorphisms of the studied fragments were explored using single strand conformational polymorphism (SSCP) and DNA sequencing analysis. In both Mehraban and Romanov breeds, three different banding patterns (AA, AG and GG genotypes) along with a single nucleotide polymorphism (g.625A>G) were identified, that deduced one amino acid substitution (p.171R>Q). Three haplotypes including ARR/ARR, ARR/ARQ and ARQ/ARQ were identified, which the most frequency (61.2 and 46.15% in Mehraban and Romanov breeds, respectively) was related to the ARR/ARR haplotype with high resistance to scrapie disease. The results of current study regarding the similarity of polymorphism in PRNp gene of domestic and foreign breeds could be used in sheep breeding programs. This study was conducted to investigate the polymorphisms of the Prion protein gene (PRNp ) exon 3 in Mehraban and Romanov sheep. Blood samples were collected from 124 sheep (85 Mahraban and 39 Romanov). After DNA extraction, polymerase chain reactions (PCR) were performed to amplify a 173 bp fragment of the PRNp gene exon 3 using a specific pair of primers. Polymorphisms of the studied fragments were explored using single strand conformational polymorphism (SSCP) and DNA sequencing analysis. In both Mehraban and Romanov breeds, three different banding patterns (AA, AG and GG genotypes) along with a single nucleotide polymorphism (g.625A>G) were identified, that deduced one amino acid substitution (p.171R>Q). Three haplotypes including ARR/ARR, ARR/ARQ and ARQ/ARQ were identified, which the most frequency (61.2 and 46.15% in Mehraban and Romanov breeds, respectively) was related to the ARR/ARR haplotype with high resistance to scrapie disease. The results of current study regarding the similarity of polymorphism in PRNp gene of domestic and foreign breeds could be used in sheep breeding programs.

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