University of Guilan Animal Production Research 2252-0872 9 3 2020 11 21 Effect of different sources of omega-3 and omega-6 calcium fat powder on performance, hatchability, serum lipids and ovarian follicles count in old broiler breeder hen Effect of different sources of omega-3 and omega-6 calcium fat powder on performance, hatchability, serum lipids and ovarian follicles count in old broiler breeder hen 1 15 4433 10.22124/ar.2020.14808.1463 FA F. Sattari Najafabadi Ph.D Student of Animal Nutrition, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran A. Mohit Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran H. Moravej Professor, Department of Animal Science, College of Agriculture and Natural Sciences, University of Tehran, Karaj, Iran H. Darmani Kuhi Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran N. Ghavi Hossein-Zadeh Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran Journal Article 2019 10 22 This study was conducted to determine the effect of different levels of omega-3 and omega-6 calcium fat powders on performance, hatchability, serum lipids, abdominal fat, and ovarian follicle count in Ross-308 broiler breeder hens (65 week-old). The experiment was performed in a repeated measurement design with 84 hens and 28 roosters with seven treatments (four replicates) during three months. The experimental groups included control group (no fat powder), 1.5 and 3% of animal omega-3 calcium fat powder of fish oil origin (ω3F), plant omega-3 calcium fat powder of linseed oil origin (ω3L), and omega-6 calcium fat powder of soybean oil origin (ω6S). The results showed that the highest average of HDEP was belonged to the experimental groups containing 1.5% ω3F (61.98), and ω3L (60.25) without significant difference with together (<em>P</em>> 0.05). However, among experimental groups, the highest egg mass (44.78 g/h/d) was obtained from consumption of 1.5% ω3F (<em>P</em><0.05). The highest concentrations of triglycerides (1806.8 mg/dL), cholesterol (206.25 mg/dL), and low density lipoprotein (244/25 mg/dL) of serum were belonged to control group that was significantly different from other experimental groups (<em>P</em><0.05). The percentage of hatchability of fertile eggs was improved by consuming diets containing fat powder, except 3% ω6S, when compared to control (<em>P</em><0.05). Overall, considering the production performance, hatchability and economic explanation of diets, it seems that addition of omega-3 calcium fat powder at the level of 1.5% (ω3F and ω3L) is affordable and can have a positive effect on old broiler breeder hens. This study was conducted to determine the effect of different levels of omega-3 and omega-6 calcium fat powders on performance, hatchability, serum lipids, abdominal fat, and ovarian follicle count in Ross-308 broiler breeder hens (65 week-old). The experiment was performed in a repeated measurement design with 84 hens and 28 roosters with seven treatments (four replicates) during three months. The experimental groups included control group (no fat powder), 1.5 and 3% of animal omega-3 calcium fat powder of fish oil origin (ω3F), plant omega-3 calcium fat powder of linseed oil origin (ω3L), and omega-6 calcium fat powder of soybean oil origin (ω6S). The results showed that the highest average of HDEP was belonged to the experimental groups containing 1.5% ω3F (61.98), and ω3L (60.25) without significant difference with together (<em>P</em>> 0.05). However, among experimental groups, the highest egg mass (44.78 g/h/d) was obtained from consumption of 1.5% ω3F (<em>P</em><0.05). The highest concentrations of triglycerides (1806.8 mg/dL), cholesterol (206.25 mg/dL), and low density lipoprotein (244/25 mg/dL) of serum were belonged to control group that was significantly different from other experimental groups (<em>P</em><0.05). The percentage of hatchability of fertile eggs was improved by consuming diets containing fat powder, except 3% ω6S, when compared to control (<em>P</em><0.05). Overall, considering the production performance, hatchability and economic explanation of diets, it seems that addition of omega-3 calcium fat powder at the level of 1.5% (ω3F and ω3L) is affordable and can have a positive effect on old broiler breeder hens. https://ar.guilan.ac.ir/article_4433_46e6c868fb45fcf51affb7d4ba8d63f8.pdf