Comparing antibacterial effect of different extracts of chicory (Cichorium intybus L.) and its effects on nutrient digestibility and gastrointestinal microflora population in broiler chicks exposed to heat stress

Document Type : Research Paper

Authors

1 Ph.D student, Faculty of Animal Science, Agriculture and Natural Resources University of Gorgan; Scientific Affair of Animal Science Department, Gonbad Kavoos University, Gonbad, Iran

2 Associate Professor, Department of Animal Science, Agriculture and Natural Resources University of Gorgan, Gorgan, Iran

3 Assistant Professor, Department of Chemistry, Agriculture and Natural Resources University of Gorgan, Gorgan, Iran

4 Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

This experiment was conducted to investigate the effect of chicory extract in the diet of broiler chicks in a completely randomized design with 5 dietary treatments, 4 replicates of 20 broiler chicks (1 d old) in each. Treatments consisted of a corn diet - soy as a basal diet (control) and basal diet containing three levels of 150, 250 and 350 mg ethyl acetate extract of chicory per kg and probiotic the recommended amount per kilogram of feed. Chicory was extracted using four different solvents (chloroform, ethyl acetate, methanol and water) and the most effective extracts in vitro antibacterial properties were determined by disk diffusion method and by measuring the diameter of inhibition zone. The most antibacterial effect against E.coli was observed to the ethyl acetate extract. Effects of ethyl acetate extract were investigated on nutrient digestibility and microbial population of the gastrointestinal tractof broilers under heat stress. To determine the digestibility of dry matter, crude protein and crude fat feed sampling and ileum with chromium oxide marker was used. The results showed that the use of the chicks chicory extract reduced the number of E. coli bacteria and increase the number of lactobacilli in the digestive tract. Digestible of protein and fat under the influence of chicory extract showed a significant increase. In the digestibility of dry matter in different treatments were not affected by treatments (P<0.05).It is concluded that improvement in the microflora and digestibility was expected with the use of ethyl acetate extract.

Keywords


Afsharmanesh M., Sadaghi B. and Silversides F. G. 2013. Influence of supplementation of prebiotic, probiotic, and antibiotic to wet-fed wheat-based diets on growth, ileal nutrient digestibility, blood parameters, and gastrointestinal characteristics of broiler chickens. Comparative Clinical Pathology, 22: 245-251.
Alzueta C., Rodríguez M., Ortiz L., Rebolé A. and Treviño J. 2010. Effects of inulin on growth performance, nutrient digestibility and metabolisable energy in broiler chickens. British Poultry Science, 51: 393-398.
Akbarian A., Golian H., Kermanshahi R., Farhoosh A., Raji R., De S., Smet S. and Michiels. J. 2013. Growth performance and gut health parameters of finishing broilers supplemented with plant extracts and exposed to daily increased temperature. Spanish Journal of Agricultural Research, 11(1): 109-119.
AOAC. 2005. Association of official analytical chemists, official methods of analysis. 18th (Ed). Maryland, USA.
Bailey M. T., Lubach G. R. and Coe C. L. 2004. Prenatal stress alters bacterial colonization of the gut in infant monkeys. The Journal of pediatric Gastroenterology and Nutrition, 38: 414-421.
Biggs P., Parsons C. M. and Fahey G. C. 2007. Several oligosaccharides on growth performance, nutrient digestibility, and cecal microbial populations in young chicks. Poultry Science, 86: 2327-2336.
Ceksteryte V., Kazlauskas S. and Racys J. 2006. Composition of flavonoids in Lithuanian honey bee bread. Biologija, 2: 28-33.
Chen Y. C., Nakthong C. and Chen T. C. 2005. Improvement of laying hen performance by dietary prebiotic chicory oligofructose and inulin. International Journal of Poultry Science, 4: 170-178.
Chow J. 2002. Probiotics and prebiotics: A brief overview. Journal of Renal Nutrition, 12: 76-86.
Engberg R. M., Hedemann M. S. Leser T. D. and Jensen B. B. 2000. Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broilers. Poultry Science, 79: 1311–1319.
Farag R. S., Badei A. Z. M. A., Hewedi F. M. and El-Baroty G. S. A. 1989. Antioxidant activity of some spice essential oils on linoleice and oxidation in aqueous medisa. Journal of the American Oil Chemists' Society, 66: 792-799.
Finke B., Stahl B., Pritschet M., Facius D., Wolfgang J. and Boehm G. 2002. Preparative continuous annular chromatography (P-CAC) enables the large-scale fractionation of fructans. Journal of Agricultural and Food Chemistry, 50: 4743-4748.
Guido F., Antognoli E. and Morelli I. 2001. Two flavonoids and other compounds (Ed.), Longman Scientific and Technical, Essex. Nutrition, 85-117
Hai L., Rong D. and Zhang Z. Y. 2000.The effect of thermal environment on the digestion of broilers. Journal of Animal Physiology and Animal Nutrition, 83: 57-64.
Haj Ayed M., Laamari Z. and Rrkik. B. 2004. Effects of incorporating an antibiotic Avilamycin and probiotic activis in broiler diets Proceeding, Western section, American Society of Animal Science, 55: 237-240.
Herandez F., Madrir J. and Garcia V. 2004. Influence of two plant extracts on broiler performance, digestibility and digestive organ size. Poultry Science, 83: 169-174.
Hinton A. J. R., Buhr R. J. and Ingram K. D. 2000. Reduction of Salmonella in the crop of broiler chickens subjected to feed withdrawal. Journal of Poultry Science, 79: 1566-1570.
Huang K. H., Ravindran V., Li X. and Bryden W. L. 2005. Influence of age on the apparent ileal amino acid digestibility of feed ingredients for broiler chickens. British Poultry Science, 46: 236-245.
Izadi shoorab H., Arshaami J., golian A., Raji A. R. 2013.  Effects of chicory root powder on growth performance and histomorphometry of jejunum in broiler chicks, Veterinary Research Forum, 4: 169-174
Jamroz D. A., Wiliczkiewicz T., Wertelecki J. 2005. Use of active substances of plant origin in chicken diets based on maize and locally grown cereals. British Poultry science, 46: 485-493.
Jang L. S., Ko Y. H., Kang S. Y. and Lee C. Y. 2007. Effect of commercial essential oils on growth performance, digestion enzyme activity and intestinal microflora population in broiler chickens. Animal Feed Science and Technology, 134: 304-315.
Jin L. Z., Ho Y. W., Abdullah N. and Jalaludin S. 1997. Probiotic in poultry: modes of action. Worlds Poultry Science, 53: 351-368.
Klien H. 2002. Poultry feeding programs without antibiotic. From http://www.afac.se/pdf.
Larbier Z. N., Chagnau A. M. and Geraert P. A. 1993. Influence of ambient temperature on true digestibility of protein and amino acids of rapeseed and soybean meals in broilers. Poultry science, 72: 289-295.
Lee K. W., Everts H., Kappert H. J., Frehner M., Losa R. and Beynen A. C. 2003b. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, 44: 450–457.
Lin S. Y., Hung A. T. Y. and Lu. J. J. 2011. Effects of supplement with different level of Bacillus coagulans as probiotic on growth performance and intestinal microflora populations of broiler chickens. Journal of Animal and Veterinary Advances, 10: 111-114.
Mehmood N., Zubaır M., Rızwan K. and Rasool N. 2012. Antioxidant, Antimicrobial and phytochemical analysis of Cichorium intybus seeds extract and various organic fractions. Iranian Journal of Pharmacy Research, 11(4): 1145–1151.
Murry A. C., Hinton A.  and Buhr R. J. 2006. Effect of botanical probiotic containing lactobacilli on growth performance and populations of bacteria in the ceca, cloaca, and carcass rinse of broiler chickens. International Journal of Poultry Science, 5 (4): 344-350.
National Committee for Clinical Laboratory Standards (NCCLS). 1997. Performance Standards for Antimicrobial Disc Susceptibility Test (5th ed.) approved Standard. M2-A6.
Ross R. P., Morgan S. and Hill C. 2002. Preservation and fermentation: Past, present and future. International Journal of Food Microbiology, 79: 3-16
Ravindran V., Cabhug S., Ravindran G. and Bryden W. L. 1999. Influence of microbial phytase on apparent ileal amino acid digestibility of feedstuff for broilers. Poultry Science, 78: 699-706.
Roberfroid M. B., Vanloo J. A. E. and Gibson. G. R. 1998. The bifidogenic nature of chicory inulin and its hydrolysis products. Journal of Nutrition, 128: 11–19.
SAS Institute. 2006. SAS/STAT User’s Guide. Version 9.1. SAS Inst. Inc., Cary, NC.
Si W., Gong J., Tsao R., Zhou T., Yu H., Poppe C., Johnson R. and Du Z. 2006. Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria. Journal of Applied Microbiology, 100: 296-305.
Wallis I. R. and Balnave D. 1984. The influence of environmental temperature, age and sex on the digestibility of amino acids in growing broiler chickens. British Poultry Science, 25: 401-407
Williams C. H., David D. J and Iismaa O. 1962. Determination of chromium in feed and feces samples. The Journal of Agricultural Science, 59: 381-385.
Williams C. M. 1999. Effects of inulin on lipid parameters in humans. Journal of Nutrition, 129: 1471-1473.
Zhang Z. F. and Kim I. H. 2014. Effects of multistrain probiotics on growth performance, apparent ileal nutrient digestibility, blood characteristics, cecal microbial shedding, and excreta odor contents in broilers. Poultry Science, 93: 364–370.