Comparison of lactoferrin and TLR4 genes expression in milk somatic cells of Holstein and Guilan native cattle and their crossbreds

Document Type : Research Paper

Authors

1 MS.c student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan

2 Assistant professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan

3 Ph.D student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan

4 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan

Abstract

The aim of the present study was to compare the expression of lactoferrin and TLR4 genes in cow milk somatic cells to investigate genetic differences effects and crossbreeding on the gene expression of the immune system as well as the effect of mastitis. Total RNA was extracted from fresh milk cells of 3 healthy Holstein, 3 Guilan Native and 3 F1 crossbred cattle belong to the National Animal Breeding Center of Iran and 3 Holstein with mild symptoms of clinical mastitis. After cDNA synthesis genes expression was measured using Real-time PCR relative to the reference gene GAPDH. The results showed while in crossbred cows the average expression of both genes was higher than parents (P<0.05), but the standard deviation of the lactoferrin gene expression was high in crossbred group. TLR4 gene expression in Holstein with clinical mastitis was significantly higher than Holstein without clinical mastitis (P<0.05), but no significant difference was observed in lactoferrin gene expression between healthy and diseased cattle (P>0.05). This study indicated that while there was non-significance difference between Guilan native and Holstein, but the crossbreeding clearly affected on the expression of two genes associated with genetic resistance.

Keywords

References

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Animal Production Research
Volume 5, Issue 2
September 2016
Pages 61-71

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