Identification of Myostatin gene polymorphism by PCR-RFLP and its association with some morphological traits in some horse breeds

Document Type : Research Paper

Authors

1 Assistant Professor, Animal Science Group, Agriculture Faculty, Islamic Azad University, Karaj Branch, Karaj, IRAN

2 Associate Professor, Animal Science Group, Agriculture Faculty, Islamic Azad University, Karaj Branch, Karaj, IRAN

Abstract

The morphological traits are polygenic and are affected by environment and polygenes, at the sametime. In this study, the Myostatin gene polymorphism in promoter region (MSTN) and its association with some morphological traits were studied in 120 random selected horses of Oldenburg, Turkmen, Thoroughbred and Caspian pony. After extraction of genomic DNA, PCR was done for 204 bp region of MSTN. The PCR-RFLP method according by SscpI was done for sample genotyping. The results showed that there are two TT and CT genotypes and T and C alleles for MSTN promoter locus in research samples. The MSTN genotypes had no significant effect on none of the chest width, height, hip and neck length traits across all studied breeds. The neck circuit for Thoroughbred and Caspian pony were 114.26 and 87.59 cm in TT genotype and 109.87 and 81.38 cm in CT genotype, respectively and differences were significant. In Oldenburg and Turkmen the TT genotype showed better performances for neck circuit however differences were no significant. In conclusion, there was polymorphism in the studied promoter region in all studied breeds and the MSTN polymorphism, as a candidate gene, can be used to identify the gentic potential differences in horse breeds.

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