Effects of In- Ovo injection of beta-hydroxy-beta-methyl butyrate and glucose on intestinal morphology and growth performance of broiler chickens

Document Type : Research Paper

Authors

1 Associate Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Graduated M.Sc Student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

This study was conducted to investigate the effects of in- Ovo injection of beta-hydroxy-beta-methyl butyrate and glucose on intestinal morphology and growth performance of broiler chickens. On day 18 of incubation 400 Ross 308 breeder fertile eggs were weighed and distributed into 4 treatments in a completely randomized design. 1 ml of an in-Ovo fed solution including: 1) 150 g glucose in 5 g/l Nacl(GLU), 2) 1 g beta-hydroxy-beta-methyl butyrate in 5g/l Nacl (HMB)and 3) 5g/l NaCl was injected into amniotic fluid of eggs(PC). The control group remained non-injected (NC). Upon hatch, chicks were weighed and transferred to the experimental house and reared to 42 days. Feed intake and daily gain were recorded and FCR was calculated based on observed data. Glucose and HMB lead to significant reduction in hatchability (P<0.05). Hatched chicks Body weight and body weight to egg weight ratio produced from treated eggs were significantly higher than that control (P<0.05), though FCR remained with no significant difference. In ovo administration of glucose and HMB appeared with no significant differences on FCR. On day 21 post-hatch, compared to control group, villi height and surface area of hatched chicks from egg injected with glucose and HMB, lead to significantly increase(P<0.05).
Conclusion was that, in- Ovo injection of Glucose and HMB can improve post-hatch chick performance and development parameters such as: day old chick weight, chick to egg weight ratio as well as broiler chicken intestinal morphology.

Keywords


Bhattacharyya A., Majumdar S., Bhanja S. K., Mandal A. B., Dash B. B. and Agarwal S. K. 2007. Effect of in ovo injection of glucose on growth, immunocompetence and development of digestive organs in turkey poults. 16th European Symposium on Poultry Nutrition, August 26-30, 2007, Strasbourg, France.
Bhanja S. K., Mandal A. B., Agarwal S. K. and Majumdar S. 2008. Effect of in ovo injection of glucose on the hatch weight and blood biochemical parameters of the day-old broiler chicks Indian. Animal Science, 78(8): 869-872.
Ferket P. R. 2006. Incubation and in ovo nutrition affects neonatal development. 33rd Annual Carolina Poultry Nutrition Conference, September 26, 2006, Sheraton Imperial Hotel, North Carolina.
Foy O.T. 2005. The biochemical and molecular effects of amnionic nutrient administration, “in ovo feeding” on intestinal development and function and carbohydrate metabolism in turkey embryos and poults. PhD Dissertation. Department of Poultry Science North Carolina State University.
Foy O.T., Ashwell C., Uni Z. and Ferket P. R. 2009. The effect of intra-amnionic feeding of arginine and/or β-Hydroxy-β-Methylbutyrate on jejunal gene expression in the turkey embryo and hatchling. Poultry Science, 8(5): 437-445
Foy O. T., Uni Z. and Ferket P. R. 2006. Effect of in ovo feeding egg wight protein, β-Hydroxy-β-Methylbutyrate, and carbohydratee on status and neonatal growth of turkeys. Poultry Science, 85: 1185-1192.
Geyra A., Uni Z. and Sklan D. 2001a. Enterocyte dynamics and mucosal development in the posthatch chick. Poultry Science, 80: 776-782.
John T. M., George J. C. and. Moran E. T. 1988. Metabolic changes in pectoral muscle and liver of turkey embryos in relation to hatching: influence of glucose and antibiotic treatment of eggs. Poultry Science, 67: 463-469.
Nissen S. L. and Abumrad N. N. 1997. Nutritional role of the leucine metabolite β-hydroxy-β-methylbutyrate (HMB). Journal of Nutritional Biochemistry, 8: 300–311.
Nissen S., Jr. Fuller J. C, Sell J., Ferket P. R and. Rives D.B. 1994. The effect of β-methyl-β-hydroxybutyrate on growth, mortality, and carcass qualities of broiler chickens. Poultry Science, 73(1):137-155.
Nissen S., Sharp R. L., Panton L., Vukovich M., Trappe S. and Jr Fuller J. C. 2000. β -Hydroxy- β -Methylbutyrate (HMB) supplementation in humans is safe and may decrease cardiovascular risk factors. American Society for Nutritional Sciences, 130: 1937–1945.
Noy Y. and Sklan D. 1999a. Different types of early feeding and performance in chicks and poults. Journal of Applied Poultry Research, 8: 16-24.
Noy Y. and. Sklan D. 1998. Yolk utilization in the newly hatched poult. British Poultry Science, 39: 446-451.
Ostaszewski P. and Nissen S. 1988. Effect of hyperglucagonemia on whole-body leucine metabolism in immature pigs before and during a meal. American Journal of Physiology, 254:372–377.
Sklan D. 2001. Development of the digestive tract of poultry. Worlds Poultry Science Journal, 57: 415–427.
Tako E., Ferket P. R. and Uni Z. 2004. Effect of in ovo feeding of carbohydrates and β-Hydroxy-β-Methyl butyrate and carbohydratee on the development of chicken intestine. Poultry Science. 83: 2023-2028.
Uni Z. and Ferket P.R. 2004. Methods for early nutrition and their potential. Worlds Poultry Science Journal, 60: 101-111.
Uni Z., Ganot S. and Sklan D. 1998. Posthatch development of mucosal function in the broiler small intestine. Poutry Science, 77: 75-82.
Uni Z., ferket P. R., Tako E. and kedar O. 2005. In ovo feeding improves energy status of late-term chicken embryos. Poultry Science, 84: 764-770.