Effect of Silymarin on performance and immune responses of broilers

Document Type : Research Paper

Authors

1 Graduated M.S. Student, Department of Animal Science, College of Agriculture, University of Guilan

2 Associate professor, Department of Animal Science, College of Agriculture, University of Guilan

3 Assistant professor, Department of Animal Science, College of Agriculture, University of Guilan

Abstract

The effects of different levels of Silymarin were studied on performance and immune responses in 288 one-day chicks (Cobb 500) in a completely randomized design with 6 treatments and 4 replicates and 12 observations per replicate. The treatment groups received 0 (Control), 160, 200, 240, 280 and 320 ppm of Silymarin in diet, respectively during days 3 to 42. Daily feed intake, daily body weight gain and feed conversion ratio (performance) were measured. The birds were immunized with sheep red blood cells (SRBC) on days 8 and 22 of age and serum antibody levels produced in response to SRBC were measured on days 21, 28, 35 and 42 by hemagglutination assay. Skin response to phytohemagglutinin-P (PHA-P) injected intradermally on day 16 were measured 24 and 48 h after injection. The results indicated that consumption of 200, 240, 280 and 320 ppm Silymarin reduced feed intake and feed conversion rate (P<0.05), but they did not affect on daily weight gain (P>0.05). The levels of 200, 240, 280 and 320 ppm Silymarin were increased total Anti-SRBC and IgG titer in experimental groups compared to control group (P<0.05), but IgM titer and skin response against PHA-P were not affected by Silymarin (P>0.05). It is concluded that the continuum consumption of 200, 240, 280 and 320 ppm Silymarin improved performance and increased humoral immunity in broilers.

Keywords

References

تیموری‌زاده ز.، رحیمی ش.، کریمی ترشیزی م. ا. و امیدبیگی ر. 1388. مقایسه اثر عصاره‌های آویشن(Thymus vulgaris L.)، سرخارگل(Moench. (L.) Echinacea purpurea)، سیر(Allium sativum L.) و آنتی‌بیوتیک ویرجینیامایسین بر جمعیت میکروفلور روده و سیستم ایمنی جوجه‌های گوشتی. فصلنامه علمی- پژوهشی تحقیقات گیاهان دارویی و معطر ایران، 25 (1): 39-48.
زرگری ع. 1376. گیاهان دارویی. موسسه انتشارات و چاپ دانشگاه تهران. چاپ پنجم، جلد سوم، صفحه: 24-38.
Agarwal R. Agarwal C. H., Ichikaw H. and Singh R. P. 2006. Anticancer potential of Silymarin: From bench to bed side (Review). Anticancer Research, 26: 4457-4498.
Basiglio C. L., Sanchez Pozzi E. J., Mottino A. D. and Roma M. G. 2009. Differential effects of Silymarin and its active component silibinin on plasma membrane stability and hepatocellular lysis. Chemico-Biological Interaction, 179: 297-303.
Bendich A. 1993. Physiological role of antioxidant in the immune system. Journal of Dairy Science, 76: 2789-2794.
Blevins S., Siegel P. B., Blodgett D. J., Ehrich M., Saunders G. K. and Lewis R. M. 2010. Effects of Silymarin on gossypol toxicosis in divergent lines of chickens. Poultry Science, 89: 1878-1886.
Cook N. C. and Samman S. 1996.
Flavonoids-chemistry, metabolism, cardio protective effects, and dietary sources. The Journal of Nutritional Biochemistry, 7: 66-76.
Feher J., Lang I., Nekam K., Csomos G., Muzes G. and Deak G. 1987. Effect of silibinin on the activity and expression of superoxide dismutase in lymphocytes from patients with chronic alcoholic liver disease. Free Radical Research Communical, 3: 373-377.
Gawe A., Kotonski B., Madej J. A. and Mazurkiewicz M. 2003. Effect of silymarin on chicken and turkey broilers’ rearing and the production indices of reproduction hen flocks. Medycyna Weterynaryjna, 59 (6): 517-520.
Gharagozloo M., Velardi E., Bruscoli S., Agostini, M., Di Sante M., Donato V., Amirghofran Z. and Riccardi C. 2010. Silymarin suppress CD4+ T cell activation and proliferation: Effects on NF-B activity and IL-2 production. Pharmacological Research, 61: 405-409.
Goel V., Chang C., Slama J. V., Barton R., Bauer R., Gahler R. and Basu T. K. 2002. Alkylimids of Echinacea purpurea stimulate alveolar macrophage function in normal rats. International Journal of Immunopharmacology, 2(3): 381-387.
Grasman K. A. 2010. In vivo functional tests for assessing immunotoxicity in birds (Ed.), Immunotoxicity testing: Methods and Protocols, Methods in Molecular Biology (pp. 387-397). Humana Press, Product.
Johnson V. J., Osuchowski M. F., He, Q. and Sharma R. P. 2002. Physiological responses to a natural antioxidant flavonoids mixture, Silymarin, in BALB/c mice: II Alterations on thymic differentiation correlate with changes in c-myc gene expression. Planta Medica, 68 (11): 289-296.
Kreunkinet M. and Van der Zijpp I. 1990. Effect of different dose of sheep erythrocytes on the humoral immune response of chicken lines selected for high or low antibody production. Poultry Science, 69: 608-614.
Kvasnicka F., Biba B., Sevcik R., Voldrich M. and Kratka J. 2003. Analysis of the active components of Silymarin. Journal of Chromatography, 990: 239-245.
Meroni P. L., Barcellini W., Borghi M. O., Vismara A., Ferraro G., Ciani D. and Zanussi C. 1988. Silybin inhibition of human T-lymphocyte activation. International Journal of Tissue Reactions, 10(3): 177-181.
Morazzoni P., Magistretti M. J., Giachetti C. and Zanolo G. 1992. Comparative bioavailability of Silipide, a new flavanolignan complex, in rats. European Journal of Drug Metabolism and Pharmacokinetics, 17: 39-44.
Muzes G., Deak G., Lang I., Nekam K., Gergely P. and Feher J. 1991. Effect of the bioflavonoid Silymarin on the in vitro activity and expression of superoxide dismutase (SOD) enzyme. Acta Physiologica Hungarica, 78: 3-9.
Quarantelli A., Righi F., Bonomi A., Renzi M., Bruni R. and Fusari A. 2005. La silimarina nell’alimentazione delle galline ovaiole. Congresso della Societa` Italiana, 5 Jun. Italia, pp. 463-464.
Recoquillay F. 2006. Active plant extracts show promise in poultry production. Poultry International, 45 (2): 28-30.
Schiavone A., Righi F., Quarantelli A., Bruni R., Serventi P. and Fusari A. 2007. Use of Silybum marianum fruit extract in broiler chicken nutrition: influence on performance and meat quality. Journal of Animal Physiology and Animal Nutrition, 91: 256-262.
Schumann J., Prockl J., Kiemer A. K., Vollmar A. M., Bang R. and Tiegs G. 2003. Silibinin protects mice from T cell-dependent liver injury. Journal of Hepatology, 39(3): 333-340.
Soto C., Recoba R., Barron H., Alvarez C. and Favari L. 2003. Silymarin increases antioxidant enzymes in alloxan-induced diabetes in rat pancreas. Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, 136: 205-212.
Suchy P., Strakova E., Kummer V., Herzig I. and Blechova E. 2008. Hepatoprotective effects of milk thistle (Silybum marianum) seed cakes during the chicken broiler fattening. Acta Veterinaria Brno, 77: 31-38.
Takahashi K., Mashiko T. and Akiba Y. 2000. Effect of dietary concentration of xylitol on growth in male broiler chicks during immunological stress. Poultry Science, 79: 743-747.
Tedesco D., Steidler S., Galletti S., Tameni M., Sonzogni O. and Ravarotto L. 2004. Efficacy of Silymarin-phospholipid complex in reducing the toxicity of aflatoxin B[1] in broiler chicks. Poultry Science, 83(11): 1839-1843.
Urbanczyk J., Hanczakowska E. and Swiatkiewicz M. 2002. Herb mixture as an antibiotic substitute in pig feeding. Medycyna Weterynaryjna, 58 (11): 887-889.
Van der zijpp, A. J., Scott T. R. and Glick B. 1986. The effect of different routes of antigen administration on the humoral immune response of the chicks. Poultry Science, 65: 809-811.
Vogle G. 1979. Studies on pharmacodynamics, site and mechanism of action of silymarin the antihepatotoxic principale from Silybum marianum (L.) Gaert. Arzneim Forsch, 25: 179-185.
Wilasrusmee C., Kittur S., Shah G., Siddiqui J., Bruch D. and Wilasrusmee S. K. 2002. Immunostimulatory effect of Silybum marianum (milk thistle) extract. Medical Science Monitor, 8(11): 439-443.
Animal Production Research
Volume 2, Issue 3 - Serial Number 3
December 2013
Pages 49-58

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