Effect of replacing alfalfa with subabul (Leucaena leucocephala) pod on digestibility, in vitro fermentation and in situ degradability in cow and buffalo

Document Type : Research Paper

Authors

1 MS.c Graduated student, College of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

2 Associate Professor, College of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

Abstract

This experiment was conducted to investigate effect of replacing alfalfa with subabul pod (0, 9 and 18 % of DM) on digestibility, in vitro fermentation and ruminal degradability in fistulated cow and buffalo. Fermentation of experimental diets was determined by gas production, digestibility by tilly and terry and degradability by in situ (4 replicates per diet) and the data were analyzed by split plot design. Digestibility of dry mater, NDF and ADF of diets containing different levels of subabul pod were not different in cow and buffalo (P>0.05). But potential of gas production in diets containing subabul pod was lower than control diet (P<0.05). However, replacing alfalfa with subabul pod didn’t affect gas production rate, microbial biomass and other fermentative parameters in cow and buffalo (P>0.05). Cell wall degradability was greatest for control treatment (in buffalo 23.9 % and cow 35.9 %, respectively) and the lowest value was for diet containing 18 % subabul pod (in buffalo 64.1% and cow 64.3%, respectively) (P<0.05). Replacement of alfalfa with subabul pod did not influence degradability parameters in cow and buffalo (P>0.05). According the result, replacement of alfalfa hay with subabul pod in cow and buffalo diet, didn’t affect in vitro digestibility and gas production parameters, but improved in situ ruminal degradability. Therefore, it seems 50 % subabul pod can be used instead of alfalfa in cow and buffalo nutrition.

Keywords

Main Subjects


Abubakr A. R., Alimon A. R., Yaakub H., Abdullah N. and Ivan M. 2013. Digestibility, rumen protozoa and ruminal fermentation in goats receiving dietary palm oil by-products. Journal of the Saudi Society of Agricultural Sciences, 1-8.
Aref Ibrahim M. 2005. Performance of Leucaenaleucocephala and Albizialebbeck trees under low irrigation water in the field. Journal of Agriculture Sciences, 24 (10): 5627-5636.
Arzani H., Zohdi M., Fisher E., ZaheddiAmiri G. H., Nikkhah A. and Wester. A. D. 2004. Phenological effects on forage quality of five grass species.  Journal of Range Management, 57: 624-630.
Babayemi O. J., Demeyer D. and Fievez V. 2004. In vitro rumen fermentation of tropical browse seeds in relation to their content of secondary metabolites. Journal of Animal  and Feed Sciences, 13(1): 31-34.
Bhatia S. K., Kumar S. and Sangowan, D. C. 2003. Nutritional microbiology and digestive physiology of buffalo and cattle. Teaching Manual. Department of Animal Nutrition. CCS HAU. Hisar, P: 42-44.
Blummel M., Makkar H. P. S. and Becker K. 1997. In vitro gas production- a technique revisited. Journal of Animal Physiology and Animal Nutrition, 77: 24-34.
Chaudhary P. P., Dagar S. S. and Sirohi S. K. 2012. Comparative quantification of major rumen microbial population in Indian Cattle and Buffalo fed on wheat straws based diet. Prime Journal of Microbiology Research, 2(3): 105-108.
Dryden G. 2008. Animal Nutrition Science. CABI. p 205.
Frutos P., Hervás G., Giráldez F. J. and Mantecón A. R. 2002. Condensed tannin content of several shrub species from a mountain area in northern spain, and its relationship to various indicators of nutritive value. Animal Feed Science and Technology, 95: 215–226.
Gupta H. K. and Atreja P. P. 1999. Influence of feeding increasing levels of Leucaena leaf meal on the performance of milch goats and metabolism of mimosine and 3, 4- DHP; Animal Feed Science and Technology, 78: 159.
Islam M., Nahar T. N. and Islam M. R. 1995. Productivity and nutritive value of leucaena leucocephala for ruminant nutrition. Bangladesh Livestock Research Institute, 8(3): 213-217.
Melaku S., Peters K. J. and Tegegne A. 2003. In vitro and in situ evaluation of selected multipurpose trees, wheat bran and Lablab purpureus as potential feed supplements of tef (Eragrostistef) straw. Animal Feed Science and Technology, 108: 159-179.
Menk K. H. and Stingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28: 6-55.
Mozafaryan W. 2008. Ilam Flora, Publisher Department of Natural Resources of Ilam (pp. 597-598).
NAS (National Academy of Science). 1977. Leucaena promising forage and tree crop for the tropics. National Academy of Science. Washington. DC. P 115.
NRC. 2001. Nutrient Requirments of Dairy Cattel (7th rev. Ed.) The National Academies Press, Washington, DC.
Orskov E. R. and McDonald P. 1979. The estimation of protein degradability in the rumen fromi ncubation measurements weighed according to rate of passage. Journal of Agricultural Science, 92: 499-503.
Patra A. K., Kamra, D. N. and Agarwal N. 2010. Effects of extracts of spices on rumen methanogenesis, enzyme activities and fermentation of feeds in vitro. Journal of the Science of Food and Agriculture, 90: 511–520.
Russell J. B., Strovel H. J. and Chen G. 1988. Enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production. Applied and Environmental Microbiology, 54: 872-877.
Sallam S. M. A. 2005. Nutrition value assessment of the alternative feed researches by gas production and rumen fermentation in vitro. Research Journal of Agriculture and Biological Science, 1(2): 200-209.
Singh S., Pradhan K. and Bhatia S. K. 1994. The effect of trans-inoculation of rumen contents on microflora concentration in the rumen of cattle and buffalo. Indian Journal of Animal Nutrition, 11: 133.
Tilly J. M. A.  and Terry R. A. 1963. A two stage technique for the indigestion of forage crops. Journal of British Grassland Society, 18: 104-111.
Zhang Y., Gao W. and Meng Q. 2006. Fermentation of plant cell walls by ruminal bacteria, protozoa and fungi and their interaction with fibre particle size. Archives of Animal Nutrition, 61 (2): 114-125.