Rumen degradability of xylose-treated soybean meal determined with nylon bag Technique (in situ)

Document Type : Research Paper


1 Former M.Sc. Student, Department of Animal, Science, Islamic Azad University of Karaj

2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Zanjan University


A ruminal in situexperiment using three ruminally fistulated multiparous non-lactating dairy cows was conducted to determine dry matter (DM) and crude protein (CP) degradation of soybean meal and xylose-treated soybean meal. Samples were suspended in the rumen of cows for 0, 2, 4, 8, 16, 24 and 48 h. Xylose-treated  soybean meal had higher soluble digestible fraction (a) of DM than soybean meal (21% vs. 27%). Soluble digestible fraction (a) CP of xylose-treated soybean meal was considerably lower than that of soybean meal (3% vs. 20%). Xylose-treated soybean meal showed higher slowly digestible fraction (b) CP than soybean meal (84% vs. 78%). Effective degradability of CP at outflow rates of k= 0.05 and k= 0.08 h-1 was significantly lower for xylose-treated soybean meal than soybean meal (27 and 20% vs. 62 and 53%). Results from this study showed that xylose-treated soybean meal had lower available protein for rumen microorganisms compared with soybean meal.


Adrian J. 1974. Nutritional and physiologic consequences of the Maillard reaction. World Review of Nutrition
and Dietetics, 19:34-71.
AOAC. 1990. Official Methods of Analysis. 15th ed. Assoc. Offic. Anal. Chem., Arlington, VA.
Atwal A.S., Milligan L.P. and Young B.A. 1974. Effects of volatile fatty acid treatment on the protection of
protein in the rumen
. Canadian Journal of Animal Science, 54: 393-401.
Borucki Castro S.I., Phillip L.E., Lapierre H., Jardon P.W. and Berthiaume R. 2007. Ruminal Degradability and
Intestinal Digestibility of Protein and Amino Acids in Treated Soybean Meal Products.
Journal of Dairy
90: 810-822.
Can A. and Yilmaz A. 2002. Usage of xylose or glucose as non-enzymatic browning agent for reducing ruminal
protein degradation of soybean meal. Small Ruminant Research, 46 : 173–178.
Chalupa W. 1974. Nitrogen metabolism in ruminant animals. In: Proceedings of Guelph Nutritional Conference
on Feed Manufacturers.
Journal of Dairy Science, 58: 1198–1218.
Gonzalez J., Ouarti M., Rodr´
ıguez C.A. and Alvir M.R. 2006. Effects considering the rate of comminution of
particles and microbial contamination on accuracy of in situ studies of feed protein degradability in
ruminants. Animal Feed Science and Technology, 125: 89–98.
Gonzalez J., Andres S., Rodr´
ıguez C.A. and Alvir M.R. 2002. In situ evaluation of the protein value of soybean
meal and processed full fat soybeans for ruminants. Animal Research, 51: 455–464.
Harstad O.M. and Prestlokken E. 2000. Effective rumen degradability and intestinal indigestibility of individual
amino acids in solvent-extracted soybean meal (SBM) and xylose-treated SBM (SoyPass) determined
in situ.
Animal Feed Science and Technology, 83: 31–47.
Kamalaka A., Canbolatb O., Gurbuza Y. and Ozaya O. 2005. In situ ruminal dry matter and crude protein
degradability of plant- and animal-derived protein sources in Southern Turkey. Small Ruminant Research,
58: 135–141.
Lynch G.L., Berger L.L. and Fahey J. 1987. Effects of ethanol, heat, and lipid treatment of soybean meal on
nitrogen utilization by ruminants.
Journal of Dairy Science, 70: 90–97.
M'hamed D., Faverdin P. and Verite R. 2001. Efeects of the level and source of dietary protein on intake and
milk yield in dairy cows. Animal Research, 50: 205-211.
Nakamura T., Klopfenstein T.J., Gibb D.J. and Britton R.A. 1994. Growth efficiency and digestibility of heated
protein feed to growing ruminants. Journal of Animal Science, 72: 774–782.
Nakamura T., Klopfenstein T.J., Owen F.G., Britton R.A., Grant R.J. and Winowiski T.S. 1992.
Nonenzymatically browned soybean meal for lactating dairy cows.
Journal of Dairy Science, 75: 3519-3523.
Orskov E.R. and McDonald I. 1979. The estimation of protein degradability in the rumen from incubation
measurements weighted according to rate of passage. Journal of Agricultural Science, 92: 499-503.
Sadeghi A.A., Nikkhah A., Shawrang P. and Shahrebabak M.M. 2006. Protein degradation kinetics of untreated
and treated soybean meal using SDS-PAGE. Animal Feed Science and Technology, 126: 121–133.
SAS Institute. 1997. SAS/STAT User’s Guide: Statistics. Version 6.12. SAS Institute, Inc., Cary, NC.
Subuh A.M.H., Rowan T.G. and Lawrence T.L.J. 1996. Effect of heat or formaldehyde treatment on the rumen
degradability and intestinal tract apparent digestibility of protein in soybean meal and in rapeseed meal of
different glucosinolate content. Animal Feed Science and Technology, 57: 257-265.
Tuncer S. and Sacakli D.P. 2003. Rumen degradability characteristics of xylose treated canola and soybean
meals. Animal Feed Science and Technology, 107: 211–218.
Vander Aar P.J., Berger L.L. and Fahey J. 1982. The effects of alcohol treatments on solubility and in vitro and
in situ digestibilities of soybean meal protein. Journal of Animal Science, 55: 1179–1189.
Van Soest P.J. 1982. Nutritional Ecology of the Ruminant. O&B Books Inc., Corvallis, OR, pp. 114–117.
Waltz D.M. and Stern M.D. 1989. Evaluation of various methods for protecting soya-bean protein from
degradation by rumen bacteria. Animal Feed Science and Technology, 25: 111-122.
Windschitl P.M. and Stern M.D. 1988. Evaluation of calcium lignosulfonate-treated soybean meal as a source of
rumen protected protein for dairy cattle. Journal of Dairy Science, 71: 3310-322.
Winowiski T.S. and Stern M.D. 1987. Identification of process factors affecting degradability of lignosulfonatetreated soybean meal by rumen microbes. Journal of Animal Science, 65(suppl.1): 468.