Effect of the addition of egg yolk to skim milk extender and cold shock on ram coated spermatozoa under cold liquid storage

Document Type : Research Paper

Authors

1 Graduate M.Sc. Student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

This experiment was conducted to determine the effect of egg yolk levels and cold shock on coated spermatozoa by using 2 × 5 factorial arrangement of the ten treatments and 5 replicates under a repeated measure randomize design. At 5 sessions, semen was collected from three ram by artificial vagina contact with a tube containing Tris- fructose-egg yolk 15%. Samples were pooled, centrifuged and removed supernatant. Aliquot was split into two groups and each one was split into 5 subgroups and after that it was added egg yolk 0, 5, 10, 15 and 20%. First group was encountered with cold shock and second group was gradually cooled up to 5◦C then samples were incubated for 72 h. Progressive sperm motility, plasma membrane integrity, viability (by Hoechst 33258 fluorescent staining) and acrosome reaction (by PNA-Alexa flur-488) were investigated at 0, 24, 48 and 72 h. Sperm viability under cold shock and gradual cooling was higher in 5% egg yolk (67.6 ±1.47and 77.3±1.47, respectively) than 0% egg yolk (59.75±1.47 and 71.2±1.47, respectively). Sperm progressive motility, plasma membrane and acrosome integrity were improved in present of egg yolk; but, there was no difference among the treatments containing egg yolk. It was suggested that 5% egg yolk was superior to keep the function of ram coated spermatozoa for storage at 5ºC in skim milk extender.

Keywords

References

Aboagla E. M. E. and Terada T. 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology, 62: 1160–1172.
Bergeron A., Brindle Y., Blondin P. and Manjunath P. 2007. Milk caseins decrease the binding of the major bovine seminal plasma proteins to sperm and prevent lipid loss from the sperm membrane during sperm storage. Biology of Reproduction, 77: 120–126.
Bergeron A., Crete, M. H., Brindle Y. and Manjunath P. 2004. Low-density lipoprotein fraction from hen’s egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biology Reproduction, 70: 708–717.
Bergeron A. and Manjunath P. 2006. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molcular Reproduction and Development, 73: 1338–1344.
Braun J., Hochi S., Oguri N., Sato K. and Torres-Boggino F. 1995. Effect of different protein soplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa. Cryobiology, 32: 487-492.
Bucak M. N., Sariozkan S., Tuncer P. B., Ulutas P. A. and Akcadag H. I. 2009. Effect of antioxidants on microscopic semen parameters, lipid peroxidation, and antioxidant activities in angora goat semen following cryopreservation. Small Ruminant Research, 81: 90-95.
Darin-Bennett A. and White I. G. 1977. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology, 14: 466-470.
De Leeuw A. M., Den Daas G. H. E. and Woelders H.  1991. The fix vital stain method simultaneous determination of viability and acrosomal and status of bovine spermatozoa. Journal of Andrology, 12: 112-118.DE
De pauw I. M. C., Van soom A., Maes D., Verberckmoes S. and De Kruif A. 2003. Effect of sperm coating on the survival and penetrating ability of invitro stored bovine spermatozoa. Theriogenology, 59: 1109- 1122.
Drobins E. Z.,  Crowe L. M., Berger T., Anchordoguy T. J., Overstreet J. W. and  Crowe J. H. 1993. Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model. Journal of experimental zoology, 265: 432-437.
El Bagir N. M., Hama A. Y., Hamed R. M., Abd El Rahim A. G. and Beynen A. C. 2006. Lipid composition of egg yolk and serum in laying hens fed diets containing black cumin (Nigella sativa). International Journal of Poultry Science, 5: 574–578.
Farrell H. M., Jimenez-Flores R., Bleck G. T., Brown E. M., Butler J. E., Creamer L. K., Hicks C. L., Hollar C. M., Ng-Kwai-Hang K. F. and Swaisgood H. E. 2004. Nomenclature of the proteins of cows' milk sixth revision. Journal of Dairy Science, 87: 1641-1674.
Garcia-Artiga C. 1994. Test de endósmosis en ovino. In: 7th International Meeting on Animal Reproduction, Murcia, Spain, 77-81.
Gill J., Fierro S., Bentancur O. and Olivera-Muzante J. 2011. Chilled Storage of Ram Semen Improves with the Addition of Egg Yolk and Glycerol to Milk-Based Extenders. Reproduction in Domestic Animals, 46: 503–507.
Gill J., Lundeheim N., Soderquist L. and Rodriguez-Martinez H. 2003. Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology, 59: 1241–1255.
Jasko D. J., Hathaway J. A., Schaltenbrand V. L., Simper W. D. and Squires E. L. 1992. Effect of seminal plasma and egg yolk on motion characteristics of cooled stalion spermatozoa. Theriogenology, 37: 1241-1252.
Jeyendran R. S., Van-der-Ven H. H. and Zaneveld L. J. 1992. The hypoosmotic swelling test: an update. Archives of Andrology, 29: 105-116.
Jiménez-Rabadna P., Ramna M., Garca-lvarezb O., Maroto-Moralesb A., del Olmob E., Pérez-Guzmna M. D., Bisbalb A., Fernndez-Santosb M. R., Gardeb J. J. and Solerb A. J. 2012.  Effect of semen  collection method (artificial vagina vs. electroejaculation), extender and centrifugation on post-thaw sperm quality of Blanca-Celtibérica buck ejaculates. Animal Reproduction Science, 132: 88– 95.
Kulaksiz R., cebi C.  and akcay E.  2012. The effect of different extenders on the motility andmorphology of ram sperm frozen or stored at 4 °C. Turkish Journal of Veterinary and Animal Science, 36: 177-182.
Leboeuf B., Guillouet P. H., Batellier F., Bernelas D., Bonne J. L., Forgerit Y., Renaud G.  and Magistrini M. 2003. Effect of native phosphocaseinate on the in vitro preservation of fresh semen, Theriogenology, 60: 867–877.
Lusignan M. F., Bergeron A., Lafleur M. and Manjunath P. 2011. The major proteins of bovine seminal plasma interact with caseins and whey proteins of milk extender. Biology of Reproduction, 85: 457-464.
Manjunath P. 2012. New insights into the understanding of the mechanism of sperm protection byextender components. Animal Reproduction, 9: 809-815.
Manjunath P., Sairam M. R. and Uma J. 1987. Purification of four gelatinbinding proteins from bovine seminal plasma by affinity chromatography. Bioscience Reports, 7: 231–238.
Manjunath P. and Thérien I. 2002. Role of seminal plasma phospholipidbinding proteins in sperm membrane lipid modification that occurs during capacitation. Journal of Reproductive Immunology, 53: 109–119.
Marti J. I., Martı E., Cebrián-Pérez J. A. and Muiño-Blanco T. 2003. Survival rate and antioxidant enzyme activity of ram spermatozoa after dilution with different extenders or selection by a dextran swim-up procedure. Theriogenology, 60: 1025–1037.
Maxwell W. and Salamon S. 1993. Liquid storage of ram semen: a review. Reproduction Fertility and Development, 5: 613–638.
Maxwell W. M. C.  and Stojanov T. 1996. Liquid Storage of Ram Semen in the Absence or Presence of Some Antioxidants. Reproduction Fertility Development, 8: 1013-1020.
Moore A. I., Squires E. L. and Graham J. K. 2005. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology, 51: 241–249.
Olde-Clark P. and Sego R. 1992. Calcium alters capacitation and progressive motility of uterine sperm from +/+ and tw/32/+ mice. Biology of Reproduction, 47: 629-635.
Ortman K. and rodriguez-Martinez H. 1994. Membrane damage during dilution, cooling and freezig-thawing of boar spermatozoa packaged in plastic bags. Journal of Veterinary Medicine Series A, 41: 37-47.
Pagl R., Aurich J. E., Muller-Schlosser F., Kankofer M. and Aurich C. 2006. Comparison of an extender containing defined milk protein fractions with a skim milk- based extender for storage of equine semen at 5 ºC. Theriogenology, 66: 1115–1122.
Pummer A. C., Linfor J. J. and Meyers S. A. 2002. Capacitation and acrosomal exocytosis  are enhanced by incubation of stallion spermatozoa in commercial semen extenders. Theriogenology, 57: 1493-1501.
Salamon S and Maxwell W. M. C. 2000. Storage of ram semen. Animal Reproduction Science, 62: 77–111.
Santiani A., Huanca W., Sapana R., Huanca T., Sepúlveda N. and Sánchez R. 2005. Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders .Asian Journal of Andrology, 7: 303–309.
Stellflug, J. N., Cockett N. E. and  Lewis G. S. 2008. The influence of breeding intensity on above- and belown average sexual performance rams in single- and multiple-sire breeding environments. Animal Reproduction Science, 104: 248-256.
Trimeche A., Anton A. M., Renard P., Gandemer G. and Tainturie D. 1996. Quail egg yolk: a novel cryoprotectant for the freeze preservation of Poitou jackass sperm. Cryobiology, 34: 385–393.
Varisly O., Uguz C., Agca C. and Agca Y. 2009. Motility and acrosomal integrity comparision between electroejaculated and epididimal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Animal Reproduction Science, 110: 256-268.
White I. G. 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction Fertility Development, 5: 639-658.
Witte T. S. and  Schäfer-Somi S. 2007. Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Animal Reproduction Science, 102: 181–193.
Witte T. S., Schäfer-Somi S., Kuchar A.,  Mostl E., Iben C. and Aurich C. 2009. Effect of hen’s egg yolk on capacitation and acrosome reaction of diluted canine spermatozoa. Animal Reproduction Science, 110: 293–305.
Zhao Y. and Buhr M. M. 1995. Cryopreservation extenders affect calcium flux in bovine spermatozoa during temperature challenge. Journal of Andrology, 16: 278-285.

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