Effect of parent type and sex on HSP70 gene expression of silkworm in response to severe heat shock

Document Type : Research Paper

Authors

1 MSc student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Iran

2 Assistant Professor, Department of Animal Science and Department of Sericulture, Faculty of Agricultural Sciences, University of Guilan, Iran

3 Associate Professor, Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Iran

Abstract

In order to study the parents and sexes effect as genetic elements on response of silkworm larva to increased temperature, the expression of HSP70 was measured in both sexes of four silkworm parents including 110 , 104 (Chinese varieties) and 103, 107 (Japanese varieties). Larvae was exposed to 35 min of heat shock at 45°C and then sampling of larval fat body was done at zero, two, four and twenty-four hours after heat shock in the fourth day of the fifth instar. After RNA isolation and determination of HSP70 relative gene expression by qRT-PCR, results showed that the gene expression in female was 13.8 times of male larvae with high significant differences (P<0.0001). Therefore, the comparison of silkworm parents in the four times samplings were done by factorial analysis and Duncan’s test in each sex separately. HSP70 gene expression differences among varieties was highly significant (P<0.0001). 107 and 110 varieties showed the highest expression of HSP70 gene (552 and 53) among female and male silkworm larvae groups, respectively. This gene was increasingly expressed immediately after heat exposure, but there were no significant differences between the times of zero and 2 hour after heat shock. Thermal tolerance test showed that two varieties of 110 and 107 (0.63 and 0.44) had less mortality and more tolerant than 103 and 104 (0.94 and 0.74). The results showed that the 103 variety which had the lowest HSP70 expression, was also so sensitive to heat stress.

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References

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Animal Production Research
Volume 6, Issue 2
September 2017
Pages 87-97

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