Allelic polymorphism in exon 2 of the BoLA-DRB3 gene in Iranian Holstein cows

Document Type : Research Paper

Authors

1 Protessor, Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Resident in department of microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Major histocompatibility complex (MHC) of vertebrates comprises a group of genes, which plays a central role
in immune response and includes MHC- class I, II and III. The bovine MHC system is known as BoLA and its
corresponding genes are located on chromosome 23. The BoLA-DRB3.2 genes are part of the MHC class II in
cattle. These genes are highly polymorphic, found to be associated with resistance/susceptibility to infections and
also with fertility, growth and milk production parameters. Polymorphism of exon 2 of the BoLA-DRB3 gene was
detected by the PCR-RFLP method in 350 Iranian Holstein cattle. After DNA extraction, second exon of BOLADRB3
was amplified by the seminested PCR method. The fragments produced by amplifying second exon were cut
by three enzymes, RsaI, PsuI and HaeIII. In restriction fragment analysis 18, 5 and 6 different allelic patterns were
observed for RsaI, PsuI and HaeIII restriction enzymes, respectively. In total, 48 different alleles were detected
where
 
٩ out of 48 alleles were previously unreported. Allelic frequencies, genotype frequencies, expected and
observed homozygosty and heterozygosity were calculated. The other step was evaluating the frequencies of alleles
mediating resistance and susceptibility to Bovine leukosis(BLV), Foot and mouth disease (FMD), mastitis and
somatic cell count (SCC), Cystic ovarian disease, some parasitic infections and increasing or decreasing milk
production traits. The result of this study indicates that the exon 2 region of BoLA-DRB3 gene is highly
polymorphic in the Iranian Holstein cattle. Also the high presence of alleles that are sensitive to BLV, mastitis, FMD
and to some extent tick borne disease was a remarkable point. Alleles associated with susceptibility to BLV were
most frequent. Frequency of alleles associated with milk production traits was similar to previous studied
populations.

Keywords

References

Babik W. 2010. Methods for MHC Genotyping in
Non-Model Vertebrates. Molecular Ecology
Resources: 10: 237-251.
Ballingall K. T., Luyai A., Rowlands G. J., Sales J.,
Musoke A. J., Morzaria S. P., and McKeever D. J.
2004. Bovine leukocyte antigen major
histocompatibility complex class II DRB3*2703
and DRB3*1501 alleles are associated with
variation in levels of protection against Theileria
parva challenge following immunization with the
sporozoite p67 antigen. Infection and Immunity
(IAI): 72: 2738-2741.
Eveline M., and Ibeagha-Awemu P. K. 2008. A
critical analysis of disease-associated DNA
polymorphisms in the genes of cattle, goat, sheep,
and pig. Mammalian Genome: 19: 226-245.
Garcia-Briones M. M., . Russell G. C., Oliver R. A.,
Tami C., Taboga O., Carrillo E., Palma E. L.,
Sobrino F. and Glass E. J. 2000. Association of
bovine DRB3 alleles with immune response to
FMDV peptides and protection against viral
challenge. Vaccines: 19: 1167-1171.
Gibson J. P. and Bishop S. C. 2005. Use of molecular
markers to enhance resistance of livestock to
disease: a global approach. Revue Scientifique et
Technique-Office International des Epizootic:
24(1): 343-353.
Juliarena MA., Poli M., Sala L., Ceriani C., Gutierrez
S., Dolcini G., et al. 2008. Association of BLV
infection profiles with alleles of the BoLADRB3.2
gene. Animal Genetics: 39(4): 432-438.
Ledwidge S. A., mallard B. A., Gibson J. P., Jansen G.
B. and Jiang Z. H. 2001. Multi-primer target PCR
for rapid identification of bovine DRB3 alleles.
Animal Genetics: 32: 219-221.
Lewin, H. A., Russell G. C. and Glass E. J. 1999.
Comparative organization and function of the
major histocompatibility complex of domesticated
cattle. Immunological Reviews: 167: 145-58.
Maillard J. C., Berthier D., Chantal I., Thevenon S.,
Sidibe I., Stachurski F., Belemsaga D.,
Razafindraibe H. and Elsen J. M. 2003. Selection
assisted by a BoLADR/ DQ haplotype against
susceptibility to bovine dermatophilosis. Genetics
SelectionEvolution: 35: S193-S200.
Maillard J. C., Renard C., Chardon P., Chantal I., and
Bensaid A.1999. Characterization of 18 new
BoLA-DRB3 alleles. Animal Genetics: 30: 200-
203.
Martinez M.L., Machado MA., Nascimento CS., Silva
MVGB, Teodoro RL. and et al. 2006.
Association of BoLA-DRB3.2 alleles with tick
(Boophilus microplus) resistance in cattle. Genetic
and Molecular Research(GMR): 5: 513-524.
Menshad A., Eftekhar shahroodi F., Nassiry R.,
Valizadeh R. and et al. 1385. Study of genetic
polymorphism and allelic frequency of BoLADRB3.2
loci in Mazandaran Waterbuffaloes. In:
Proceeding of 9
 
thCongress of Iranian genetic
society 20-22 May. Tehran, Naghshine publication. pp. 481. (In Farsi)
Nassiry M. R., Eftekhar shahroodi F., Mosafer J.,
Norouzy A., and Javadmanesh A. 2004
Distribution of bovine
lymphocyte antigen (BoLA-DRB3) alleles in Iranian
Holstein cattle. In: Proceeding of 11
 
th Asian-
Australian Animal Production Congress.
Malaysia, pp. 266-268.
Nikbakht Gh. and Emam M. 2008. Polymorphism of
BOLA-BRB 3.2 gene in Iranian Holstein cattle by
PCR-RFLP method. Pajouhesh & Sazandegi: 77:
141-148. (In Farsi)
Rupp R., Hernandez A. and Mallard BA. 2007.
Association of Bovine Leukocyte
Antigen(BoLA)DRB3.2 with Immune Responce,
mastitis and production and type traits. Dairy
science: 90: 1029-1039.
Schaschl H., Goodman S. J. and Suchentrunk F. 2004.
Sequence analysis of the MHC class II DRB
alleles in Alpine chamois (Rupicapra r. rupicapra).
Developmental and Comparative Immunology
(DCI): 28: 265-277.
Sharif S., Mallard BA., Wilkie BN., Sargeant
JM.,Scott HM., Dekkers JCM. and Leslie KE.
1998. Associations of the bovine major
histocompatibility complex DRB3 (BoLA-DRB3)
alleles with occurrence of disease and milk
somatic cell score in Canadian dairy cattle.
Animal Genetics: 29: 185-193.
Sharif S., Mallard B. A., Wilkie B. N., Sargeant J. M.,
Scott H. M., Dekkers J. C. and Leslie K. E. 1999.
Associations of the bovine major
histocompatibility complex DRB3 (BoLA-DRB3)
with production traits in Canadian dairy cattle.
Animal Genetics: 30: 157-160.
Sharif S., Mallard B. A. and Sargeant J. M. 2000.
Presence of glutamine at position 74 of pocket 4
in the BoLA-DR antigen binding groove is
associated with occurrence of clinical mastitis
caused by Staphylococcus species. Veterinary
Immunology and Immunopathology: 76: 231-238.
Starkenburg R. J., Hansen L. B. and Kehrli M. E. and
Chester-Jones H
 
. 1997. Frequencies and Effects of
Alternative DRB3.2 Alleles of Bovine
Lymphocyte Antigen for Holsteins in Milk
Selection and Control Lines. Dairy Scinece: 80:
3411-3419.
Takeshima SN., Ikegami M., Morita M. and Nakai
Yand Aida Y. 2001. Identification of new cattle
BoLADRB3 alleles by sequence-based typing.
Immunogenetics: 53: 74-81.
Tizard, I. R. 2009. Veterinary Immunology (7
 
th ed.),
Acquired immunity: Antigen-presenting receptors
(pp. 67-76) Saunders, Philadelphia.
Udina IG, Karamysheva EE, Turkova SO, OrlovaAR
and Sulimova GE. 2003. Genetic mechanisms of
resistance and susceptibility to leukemia in
Ayrshire andBlack pied cattle breeds determined
by allelic distribution of gene BoLA-DRB3.
Russian Journal of Genetics: 39(3):306-317.
Van Eijk MJ., Stewart Haynes JA. and Lewin HA.
1992. Extensive polymorphism of the BoLADRB3gene
distinguished by PCR-RFLP. Journal
of Animal Genetic: 23(6): 483-496.
Williams JKG, Rubelik AR, Livak KJ, Rafalski JA
andTingley SV 1990. DNA polymorphisms
amplified byarbitrary primers are useful as genetic
markers. Nucleic Acid Research, 18: 6531-6535.
Xu A., Van Eijk MJ., Park C. and Lewin HA. 1993.
Polymorphism in BoLA-DRB3 exon 2 correlates
with resistance to persistent lymphocytosis caused
by bovine leukemia virus. The Journal of
Immunology: 151(12): 6977.
Yoshida T., Mukoyama H., Furuta H., Kondo Y.,
Takeshima S., Aida Y., et al. 2009. Association of
the amino acid motifs of BoLA-DRB3 alleles with
mastitis pathogens in Japanese Holstein cows.
Animal Science Journal: 80(5): 510-9.
Zanotti M, Poli G, Ponti W, Polli M, Rocchi M,
Bolzani E, Longeri M, et al., 1996. Association of
BoLA class II haplotypes with subclinical
progression of bovine leukaemia virus infection in
    Holstein-Friesian cattle. Animal Genetics: 27
(5):337-341.
Animal Production Research
Volume 1, Issue 2 - Serial Number 2
September 2012
Pages 33-41

Files

Share

How to cite

Statistics