Polymorphisms in third intron of growth hormone of Guilan native cows using PCR-RFLP markers

Document Type : Research Paper

Authors

1 Graduated MS student, Animal Science Department, University of Guilan

2 Associate Professor, Animal Science Department, University of Guilan

3 Associate Professor, Plant Breeding Department, University of Guilan

4 Researcher, Rice Research Institute of Iran

Abstract

Bovine growth hormone(bGH) gene, is a part of the multiple gene family that contains prolactin and
placental lactogens. The variations in the introns of this gene, have potential usefulness as genetic markers and
could help in the genetic improvement of populations. In ordre to investigate allele frequency of MspI in introne
3 of growth hormone gene of Guilan native cattle breed, blood samples were collected from seventy heads of
randomaly choosen cattles (cows). Genomic DNA was extracted from these samples, using modified salting out
method. Polymerase chain-reaction (PCR) procedure was used to amplify a 345 bp segment in this region and
then digested with MspI restriction enzymes. Digested samples were run on the acrylamid gel 8% to recognize
their genotype. This enzyme had two restriction sites and after digestion with MspI enzyme on that fragment and
2 allels and 3 genotypes have been observed. Frequency of MspI(+) allele was estimated to 0.4643. X2 test has
showed the equilibrium of frequency MspI in samples.

Keywords

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Animal Production Research
Volume 1, Issue 2 - Serial Number 2
September 2012
Pages 65-72

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