Effect of different levels of saponins, gallic acid and tannic acid on in vitro ruminally fermentation kinetic

Document Type : Research Paper


1 Assistant Professor of Animal Science Department, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Iran

2 Former MSc. Student of Animal Science Department, Fculty of Agriculture Science and Natural Resources, Ganbad Kavous Universty, Iran


This study was conducted in order to evaluate effect of different levels (2.5, 5 and 10 % of DM) of saponin, gallic acid and tannic acid on gas production parameters and in vitro digestibility of dry matter and organic matter, using gas production technique and batch culture. Results showed that there were significant differences among treatments on gas production and rate of gas production (P<0.05). Gas production and rate of gas production were higher for control treatment than other groups. The control treatment and treatments with different levels of saponin had the highest and lowest cumulative gas production. However, addition of different levels of saponin, gallic acid and tannic acid caused to decrease organic matter digestibility (OMD), metabolizable energy (ME) and short chain fatty acids (SCFAs), and treatment contained 10% of gallic acid had the lowest OMD, ME and SCFAs. Nitrogen-ammonia concentration was the highest (10.76 mg/dl) and lowest (5.79 mg/dl) in control treatment and treatment contained 10% of tannic acid, respectively. There were significant differences among treatments on gas production, partitioning factor and microbial crude protein (P<0.05). In general, obtained results of this study showed that using different levels of saponin, gallic acid and tannic acid had no significant effect on in vitro ruminally fermentation parameters.


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