Effect of adding different levels of propolis extracts on digestibility, gas production and in vitro ruminal fermentation parameters

Document Type : Research Paper

Authors

1 MSc. Graduated, Department of Animal Science, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad, Iran

2 Assistant Professor, Department of Animal Science, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad, Iran

Abstract

A study was conducted to evaluate the effect of adding different levels of ethanol, methanol and their 50:50 (v/v) binary mixture of propolis extract on in vitro gas production parameters, dry matter and organic matter digestibility, protozoa counts and methane production in a completely randomized design. Crude propolis sample was collected from Ramian region and its extraction was taken by ethanol, methanol and their mixture. Experimental treatments were including: 1) control containing basal diet with forage: concentrate ratio of 50:50 (without any extract), treatments 2 till 5 (control treatment + 25, 50, 100, 200 µl levels of ethanol extract, respectively), treatments 6 to 9 (control treatment + levels 25, 50, 100, 200 µl of methanolic extract, respectively), treatments 10 to 13 (control treatment + 25, 50, 100, 200 µl levels of binary solvents mixture extract, respectively). Results showed that the highest and lowest potential gas productions were related to treatment containing 200 µl and 25 µl of methanolic extract, respectively (P<0.05). Treatment containing 200 µl of binary solvents mixture extract had the highest metabolizable energy, organic matter digestibility and short chain fatty acid concentration (P<0.05). There was significant difference between treatments on dry matter and organic matter digestibility (P<0.05). Hence, treatments containing 25 and 100 µl of methanolic extract had the highest and control treatment had the lowest dry matter and organic matter digestibility, respectively (P<0.05). Using 25 µl and 200 µl levels of binary solvents mixture extract reduced Halotrish more than other levels and other extract. However, treatments containing propolis extract reduced methaneproduction remarkably. Generally, it can be concluded that propolis methanolic extract has potential to improve ruminal fermentation efficiency. However, more research is warranted to manipulate rumen microbial fermentation.

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Main Subjects

References

Alencar S. M., Oldoni T. L. C., Castro M. L., Cabral I. S. R., Costa-Neto C. M., Cury J. A., Rosalen P. L. and Ikegaki M. 2007. Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. Journal of Ethnopharma Cology, 113: 278-283.
Beuvink J. M. W. and Spoelstra S. F. 1992. Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro. Appllied Microbiology and Biotechnology, 37: 505-509.
Blümmel M., MakkarH. P. S. and BeckerK. 1997. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition, 77: 24-34.
Bonvehi J. S. and Coll F. V. 2000. Study on propolis quality from China and Uruguay. Zeitschrift für Naturforschung, 55 (9-10): 778-784.
Broudiscou L. P., Papon Y. and Broudiscou A. F. 2000. Effects of dry extracts on fermentation and methanogenesis in continuous culture of rumen microbes. Animal Feed Science and Technology, 87: 263-277.
Burdock G. A. 1998. Review of the biological properties and toxicity of propolis. Food and Chemical Toxicology, 36: 341-363.
Calsamiglia S., Busquet M., Cardozo P. W., Castillejos L. and Ferret A. 2007. Invited review: Essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science, 90: 2580-2595.
Cattani M., Franco T., Lucia B. and StefanoS. 2012. Synthetic and natural polyphenols with antioxidant properties stimulate rumen microbial growth in vitro. Animal Production Science, 52: 44-50.
Chung Y. H., Zhou M., Holtshausen L., Alexander T. W., Mcallister T. A., Guan L. L., Oba M. and Beauchemin K. A. 2012. Populations and enteric methane emissions. Journal of Dairy Science, 95: 1419-1427.
Cottica S. M., Sawaya A. C., Eberlin M. N., Franco S. L., Zeoula L. M. and Visentainer J. V. 2011. Antioxidant activity and composition of propolis obtained by different methods of extraction. Journal of the Brazilian Chemical Society, 22: 929-935.
Dehority B. A. 1993. Laboratory manual for classification and morphology of rumen ciliate protozoa. CRC press, Inc, Boca Raton. FL.
Duffield F. T. and Bagg R. N. 2000. Use of ionofores in lactating dairy cattle: a review. Canadian Veteterinary Journal. Ottawa, 41: 388-394.
Duffy C. F. and Power R. F. 2001. Antioxidant and antimicrobial properties of some Chinese plant extracts. Intrernational Journal of Antimicrobial Agents, 17: 527-529.
Fernandes L. B., Franzolin R., Franco A. V. M. and Carvalho G. 2008. Aditivos orgânicos no suplemento concentrado de bovinos de corte mant idos em pastagem. Revista Brasileira de Saúde e Produção Animal, 9: 231-238.
Fievez V., Babayemi O. J. and Demeyer D. 2005. Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acids production that requires minimal laboratory facilities. Animal Feed Science and Technology, 123-124: 197-210.
Giannenas I., Skoufos J., Giannakopoulos C., Wiemann M., Gortzi O., lalas S. and Kyriazakis I. 2011. Effects of essential oils on milk production, milk composition, and rumen microbiota in Chios dairy ewes. Journal of Dairy Science, 94: 5569-5577.
Guan H., Wittenberg K. M., Ominski K. H., Krause D. O. 2006. Efficacy of ionophores in cattle diets for mitigation of enteric methane. Journal of Animal Science, 84: 1896-1906.
Itavo C. C. B. F., Morais M. G., Costa C., Itavo L. C. V., Franco G. L., Silva J. A., Reis F. A. 2011. Addition of propolis or monensin in the diet: Behavior and productivity of lambs in feedlot. Animal Feed Science and Technology, 165: 161-166.
Iwami Y., Schachtele C. F. and Yamada T. 1995. Effect of sucrose monolaurate on acid production, levels of glycolytic intermediates, and enzyme activities of Streptococcus mutans NCTC 10449. Journal of Dental Research, 74: 1613-1617.
Jenkins T. and Vazquez-Anon M. 2007. Effects of feeding oxidized fat with or without dietary antioxidants on nutrient digestibility microbial nitrogen and fatty acids metabolism. Journal of Dairy Science, 90: 4361-4367.
Khampa S. and Wanapat M. 2007. Manipulation of rumen fermentation with organic acids supplementation in ruminants raised in the tropics. Pakistan Journal of Nutrition, 6: 20-27.
Kolankaya D., Selmanglu G., OrkunK and Salih S. B. 2002. Protective effect of Turkish propolis on alcohol induced serum lipid changes and liver injury in male rats. Food Chemistry, 78: 213-217.
Kreuzer M., Kirchgessner M. and Muller H. L. 1986. Effect of defaunation on the loss of energy in wethers fed different quantities of cellulose and normal or steam flaked maize starch. Animal Feed Science and Technology, 16: 233-241.
Lana R. P., Camardelli M. M. L., Rodrigues M. T., Eifert E. C., Oliveira M. V. M., Stradiotti J. D. and Oliveira J. S. 2007. Soybean oil and propolis in the diets of dairy goats: intake of nutrients and ruminal metabolism. Revista Brasileira de Zootecnia, 36: 191-197.
Makkar H. P. S. 2005. In vitro gas methods for evaluation of feeds containing phytochemicals. Animal Feed Science and Technology, 123: 291-302.
Menke K. H., Salewski L. A., Steingass H., Fritz D. and Schneider W. 1979. The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor. The Journal of Agricultural Science, 93: 217-222.
Mirzoeva O. K., Grishanin R. N. and Calder P. C. 1997. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiological Research, 152: 239-246.
Mohammadzadeh S., Sharriatpanahi M., Hamedi M., Amanzadeh Y., Sadat Ebrahimi S. E. and Ostad S. N. 2007. Antioxidant power of Iranian propolis extract. Journal of the Food Chemistry, 103: 729-733.
Morsy A. S., Soltan Y. A., Elzaiat H. M., Sallam S. M. A., Alencar S. M., Louvandini H. and Abdalla A. L. 2011. Effect of two types of Brazilian propolis extracts on rumen gas and methane production and truly degradability in vitro. The Middle East and North Africa Journal of Animal Science, 4: 446-456.
Morsy A. S., Soltan Y. A. Sallamb S. M. A., Kreuzer M., Alencar A. S. and Abdall A. L. 2015. Comparison of the in vitro efficiency of supplementary bee propolis extracts of different origin in enhancing the ruminal degradability of organic matter and mitigating the formation of methane. Animal Feed Science and Technology, 199: 51-60.
Oeztuerk H. and Sagmanligil V. 2009. Role of live yeasts on rumen ecosystem. DTW. Deutsche Tierärztliche    Wochenschrift, 116: 244-248.
Oezturk H., Pekcan M., Sireli M. and Fidanci U. R. 2010. Effects of propolis on in vitro rumen microbial fermentation. Ankara university veteriner fakültesi Dergisi, 57: 217-221.
Oliveira M. V. M., Lana R. P., Eifert E. C., Luz D. F., Pereira J. C., Perez J. R. O. and Vargas J. R. F. M. 2007. Effect of monensin on intake and apparent digestibility of nutrients in sheep fed diets with different crude protein levels. Revista Brasileira de Zootecnia, 36: 643-651.
Olivera M. P. 1998. Use of in vitro gas production technique to assess the contribution of both soluble and insoluble fraction on the nutritive value of forage. MSc Thesis. University of Aberdeen, Scotland.
Ørskov E. R. and McDonald I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agriculture Science, 92: 499-503.
Park Y. K., Ikegaki M. and Abreu J. A. S. 1998. Estudo da preparação dos extratos de propolis e suas aplicações. Ciēncia e Tecnologia de Alimentos, 18: 313-318.
Rispoli T. B., Rodrigues I. L., Martinsneto R. G., Kazama R., Prado O. P. P., Zeoula L. M. and Arcuri P. B. 2009.  Ruminal ciliate protozoa of cattle and buffalo fed on diet supplemented with monensin or extracts from propolis. Pesquisa Agropecuária Brasileira, 44: 92-97.
Russell J. B. and Strobel H. J. 1988. Effect of additives on in vitro ruminal fermentation: a comparison of monensin and bacitracin, another gram-positive antibiotic. Journal of Animal Science, 66: 552-558.
Sallam S. M., Abdelgaleil A. S., Bueno A. M., Nasser I. C. S., Araujo M. E. A., R. C. and Abdalla A. L. 2011. Effect of some essential oils on in vitro methane emission. Archives of Animal Nutrition, 65: 203-214.
Seidel V., Peyfoon E., Watson D. G. and Fearnley J. 2008. Comparative study of the antibacterial activity of propolis from different geographical and climatic zone. Phytotherapy Research, 22: 1256-1263.
Smith J. L., Sheffield L. G. and Saylor D. 2002. Impact of ethoxyquin on productivity of dairy cattle. Journal of Dairy Science, 85: 358.
Stack R. J. and Cotta M. A. 1986. Effect of 3-phenylpropanoic acid on growth of and cellulose utilization by cellulolytic ruminal bacteria. Applied and Environmental Microbiology, 52: 209-210.
Stockdale C. R. and Gill H. S. 2011. Effect of duration and level of supplementation of diets of lactating dairy cows with selenized yeast on selenium concentrations in milk and blood after the withdrawal of supplementation. Journal of Dairy Science, 94: 2351-2359.
Stradiotti Junior D., Queiroz A. C., Lana R. P., Pacheco C. G., Eifert E. C. and Nunes P. M. M. 2004. Effect of the propolis on amino acids deamination and ruminal fermentation. Revista Brasileira de Zootecnia, 33: 1086-1092.
Stradiotti Junior D., Queiroz A. C., Lana R. P., Pacheco C. G., Camardelli M. M. L., Detmann E., Eifert E. C., Nunes P. M. M. and Oliveira M. V. M. 2004. Effect of the propolis on the in vitro fermentation of different feedstuffs by the technique of gas production. Revista Brasileira de Zootecnia, 33: 1093-1099.
Theodorou M. K., Williams B. A., Dhanoa M. S., McAllan A. B. and France J. 1994. A simple production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48: 185-197.
Ultee A. and Kets E. J. 1999. Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus. Applied and Environmental Microbiology, 65: 4606-4610.
Van Soest P. J. 1994. Nutritional ecology of the ruminant. Cornel University Press, Ithaca, New York. 476 pp.
Winter J., Moore L. H., Dowell Junior V. R. and Bokkenheuser V. D. 1989. C-ring cleavage of flavonoids by human intestinal bacteria. Applied and Environmental Microbiology, 55: 1203-1208.
Zhou X., Meile L., Kreuzer M. and Zeitz J. O. 2013. The effect of saturated fatty acids on methanogenesis and cell viability of Methanobrevi bacter ruminantium. Archaea, http://dx.doi.org/10.1155/2013/106916.
Zhou J. H., Li Y., Zhao J., Xue X. F., Wu L. M. and Chen F. 2008. Geographical traceability of propolis by high-performance liquid-chromatography fingerprints. Food Chemistry, 108: 749-759.
Animal Production Research
Volume 7, Issue 4
March 2019
Pages 33-46

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