Investigation of gene expression and gene networks related to apoptosis in sensitive and resistant Aryan broiler breeders with RNA-Seq

Document Type : Research Paper

Authors

1 Ph.D Student, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

4 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Torbat-e-Jam University, Torbat-e-Jam, Iran

5 Associate Professor, School of Molecular and Biomedical Science, The University of Adelaide, Australia

Abstract

The designing of animal breeding program on Aryan broiler chicks without development of vital organs such as the heart, has made the chicken most susceptible for ascites syndrome. The cell apoptosis is common in ascites syndrome. Therefore, this study was done with the aim of identification of genes and genetic networks that involved in cardiac cell apoptosis among normal Aryan chickens and Aryan chickens resistant to ascites. For this purpose, two ascites and two normal samples of Aryan chicken were RNA extracted and transcriptome data were produced by the Next Generation Sequencing. The data quality and elimination of pollution were performed with FastQC and Trimmomatic softwares. In addition, the gene expression analyses were investigated using Cuffmerge, Cufflink and Cuffdiff. The results showed that there were 20034 genes in samples that 291 genes were associated with 66 biological pathways. Among this, 53 genes were associated with apoptosis pathways. Investigation of the biological process of gene ontology showed that regulation of cellular process and negative regulation of apoptosis pathways were significantly affected by chickens resistant to ascites syndrome (FDR <0.01). The gene network was extracted by 48 nodes in two clusters. The results of genes’ networking showed that MEF2A, FGF10, CDK1 and MAD2L1 genes had maximum gene association. Therefore, the ascites syndrome in Aryan chickens can be controlled focusing on these genes for breeding programs and drug designing.

Keywords

Main Subjects

References

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Animal Production Research
Volume 8, Issue 1
June 2019
Pages 53-66

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