Gene set enrichment analysis using genome-wide association study to identify genes and biological pathways associated with twinning in Baluchi sheep

Document Type : Research Paper

Authors

1 Ph.D Student, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran

2 Associate Professor, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran

3 Assistant Professor, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran

4 Assistant Professor, University of Tehran, Pardis Aboreyhan, Tehran, Iran

Abstract

Baluchi sheep is one of
the indigenous sheep breeds originating from the eastern part of Iran and it is
adapted to the severe weather conditions. The economic efficiency of sheep < br />breeding is clearly related to the reproduction efficiency of the ewes. In this
study, twinning trait was studied as an important reproductive trait. To
identify the genes associated with twinning trait, genome-wide association was
done using 91 Baluchi ewes genotyped with 50k Illumina SNP chips. For this
purpose, the extended repeatability model was used. In the next step, a gene
set enrichment analysis was implemented to identify the biological pathways
associated with the twinning. Six SNP markers on chromosomes 1, 3, 10, 15 and
25 located in CTH, ANKRD13C, SRSF11, PTGER3, LDHB, LRRC40, and KCNMA1 genes
were identified. According to pathway analysis, 25 pathways were associated
with the twinning trait. Among those pathways, the defense response biological
pathway has an important role in the ovulation. Interferons and interleukins
are the main cytokine proteins in this pathway. These cytokines create a
process such as inflammation that causes oocytes releasefrom the follicle into the oviductfor transport and fertilization. Cell adhesion and
intercellular bridge pathways were other significant pathways. Cell junction pathway
via the CX43 protein can affect the transfer of GDF9 effects to
granulosa cells. To the best of our knowledge, this is the first study that
reports indirect effects of GDF9 on twinning trait in Iranian sheep.

Keywords

Main Subjects

References

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Animal Production Research
Volume 8, Issue 2
September 2019
Pages 63-80

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