Correlation between stress and acute phase and humoral immune responses in broiler chicken

Document Type : Research Paper

Authors

1 Ph.D Student of Poultry Disease, Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran

4 Professor, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

A better understanding of the relationship between innate and adaptive immunity, as well as inflammation and immune response, can help to improve immunization methods. This study aimed to evaluate the correlations between specific immunity and inflammation. A total of 60 Ross 308 broiler chicks were divided into two groups of an equal number of birds, 30 per treatment and 10 per replicate. One group, as vaccinated, was subjected to intramuscular injection of inactivated oil emulsion of Newcastle disease vaccine on days 8 and 20. The control group administered PBS by the same route on the same days. Blood samples were collected on days 7, 10, 19, and 22. The heterophil/lymphocyte ratio was used as a stress index, serum amyloid A (SAA), and alpha 1 acid glycoprotein (AGP) for acute phase response and the level of a specific antibody for quantification of adaptive immunity. Stress index, as well as acute phase proteins, increased after vaccination (p < /em><0.05). In comparison between the control and vaccinated group, the stress index elevated in the vaccinated group to 1.5 fold (0.68% to 0.97%). Significant increase was observed for SAA (0.086 mg/L to 0.096 mg/L) at day 22 (p < /em>< 0.001) and AGP (300.63 mg/L to 600.12 mg/L) at day 10 (p < /em><0.05). SAA adversely correlated with the amounts of AGP (r = -0.229, p < /em>=0.081). Stress index correlated with SAA (r= 0.411, p < /em><0.001) and antibody levels (r= 0.313, p < /em><0.001). Stress had a significant correlation with adaptive immunity and could be used for the evaluation and monitoring of vaccine efficacy.

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References

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Animal Production Research
Volume 9, Issue 4
March 2021
Pages 23-31

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