Effect of eucalyptus leaf extract, Virginia, Barley and Basma tobacco smoke on Varroa mite of honey bee

Document Type : Research Paper

Authors

1 Graduated MSc. in Animal Physiology, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Professor, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

The present study was performed to investigate the effect of eucalyptus leaf extract and tobacco smoke on Varroa mite using 36 hives over a period of 34 days. In one experimental group, no action was taken to control Varroa mite (control) and other one (positive control) Apistan Strip were placed in hive for 34 days. In the other experimental groups, Varroa mite was controlled using Barley, Basma, Virginia leaf tobacco smoke and Eucalyptus leaf extract in the first five days (first time) and the last four days (second time) during the control period. In the eucalyptus extract treatment, 15 ml of the extract solution (0.1 ml/mg) was sprayed between frames daily. In tobacco leaf smoke treatments (Barley, Basma, Virginia), the amount of 3 g of tobacco leaf was smoked. Tick shedding (daily), the number of larvae in each frame (on days 5 and 34) and the amount of honey (after 150 days) was measured. The main effect showed that mite shedding was higher in eucalyptus leaf extract (10. 3±2.01), Apistan (9.41±2.01) and Virginia tobacco smoke (7.41±2.01) than the control (0±2.01, P

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References

حسین­زاده فشالمی ن.، زین العابدین شهادتی م.، کیانی غ.، محمدرضا صلواتی م.، زمانی پ.، مهدوی ع.، و علینژاد ر.، 1394. بررسی تنوع ژنتیکی و طبقه بندی ارقام مختلف توتون تیپ شرقی. پژوهشنامه اصلاح گیاهان زراعی، 7: 126-134.
رنجبربهادری ش.، و آذرهوش ف.، 1392. مطالعه ترکیبات گیاهی (دارچین، نعناع و اکالیپتوس) موثر در کنترل جرب قرمز طیور (درمانیسوس گالینه). تحقیقات دامپزشکی، 68: 203-208.
عالی ن.، محمودی ر.، کاظمینیا م.، حضرتی ر. و  آذرپی ف. 1396.  اسانسهای گیاهی به عنوان ترکیبات دارویی طبیعی. مجله دانشکده پزشکی دانشگاه علوم پزشکی تهران، 75: 480-487.
عبدالملکی م.، بهرام نژاد ص.، و عباسی س. 1390. بررسی اثرات ضد قارچی عصاره های برخی گیاهان علیه چهار قارچ بیماری زای گیاهی. گیاهان داوری، 10: 148- 155.
گرزین ز.، نهضتی غ.، و مروج ح.، 1394. سیستم ایمنی زنبور عسل (Apis melifera). علوم و فنون زنبور عسل ایران، 6: 22-26.
مصدق م. س.، و کمیلی بیرجندی ع. ۱۳۷۰. کنه­های زیان آور زنبور عسل. مرکز انتشارات و چاپ دانشگاه شهید چمران.
Arafa W. M., Aboelhadid S. M., Moawad A., Shokeir K. M. and Ahmed O. 2020. Toxicity, repellency and anti-cholinesterase activities of thymol-eucalyptus combinations against phenotypically resistant Rhipicephalus annulatus ticks. Experimental and Applied Acarology, 81: 265-277.
Boot W. J., Calis J. N. M. and Beetsma J. 1992. Differential periods of varroa mite invasion into worker and drone cells of honeybees. Experimental and Applied Acarology, 16: 295-301.
Bowen-Walker P. L., Martin S. J. and Gunn A. 1999. The transmission of deformed wing virus between honeybees (Apis mellifera L.) by the ectoparasitic mite Varroa jacobsoni Oud. Journal of Invertebrate Pathology, 73: 101-106.
Carlson G. R., Dhadialla T.S., Hunter R., Jansson R. K., Jany C. S., Lidert Z. and Slawecki R. A. 2001. The chemicaland biological properties of methoxyfenozide, a new insecticidal ecdysteroid agonist. Pest Management Science, 57: 115-119.
Clemente M. A., de Oliveira Monteiro C. M., Scoralik M. G., Gomes F. T., de Azevedo Prata M. C. and Daemon E. 2010. Acaricidal activity of the essential oils from Eucalyptus citriodora and Cymbopogon nar-dus on larvae of Amblyomma cajennense (Acari: Ixodidae) and Anocentor nitens (Acari: Ixodidae). Parasitology Reseacher, 107: 987-992.
de Ruijter A. and Eijnde J. V. D. 2015. Detection of varroa mites in the Netherlands using tobacco smoke. Bee World, 65: 151-154.
Goodwin R. M., Taylor M. A., McBrydie H. M. and Cox H. M. 2006. Drift of Varroa destructor infested worker honeybees to neighbouring colonies. Journal of Apicultural Research, 45: 155-156.
Gregorc A., Poganik A. and Bowen I. D. 2004. Cell death in honeybee (Apis mellifera) larvae treated with oxalic or formic acid. Apidologie, 35: 453-460.
Guzman-Novoa E., Eccles L., Calvete Y., McGowan J., Kelly P. G. and Correa-Benitez A. 2010.Varroa destructor is the main culprit for the death and reduced populations of overwintered honey bee (Apis mellifera) colonies in Ontario, Canada. Apidologie, 41: 443-450.
Haarmann T., Spivak M., Weaver D., Weaver B. and Glenn T. 2002. Effects of fluvalinate and coumaphos on queen honey bees (Hymenoptera: Apidae) in two commercial queen rearing operations. Journal of Economic Entomology, 95: 28-35.
Imdorf A., Kilchenmann V., Bogdanov S., Bachofen B. and Beretta C. 1995. Toxic effects of thymol, camphor, menthol and eucalyptol on Varroa jacobsoni oud and Apis mellifera L in a laboratory test. Apidologie, 26: 27-31.
Johnson R. M., Dahlgren L., Siegfried B. D. and Ellis M. D. 2013. Acaricide, fungicide and drug interactions in honey bees (Apis mellifera). PloS One, 8: e54092.
Johnson R. M. and Percel E. G. 2013. Effect of a fungicide and spray adjuvant on queen-rearing success in honey bees (Hymenoptera: Apidae). Journal of Economic Entomology, 106: 1952-1957.
Koleoglu G., Goodwin P. H., Reyes-Quintana M., Hamiduzzaman M. M. and Guzman-Novoa E. 2017. Effect of Varroa destructor, wounding and Varroa homogenate on gene expression in brood and adult honey bees. PloS One, 12: e0169669.
Moritz R. F. A. and Erler S. 2016. Lost colonies found in a data mine: Global honey trade but not pests or pesticides as a major cause of regional honeybee colony declines. Agriculture, Ecosystems & Environment, 216: 44-50.
Perrucci S., Marcianti F. and Cionti P. L. 1994. In vitro antifungal activity of essential oils against some isolates of Microsperum canis and M. gypseum. Planta Medica, 60: 84-87.
Ramsey S. D., Gulbronson C., Mowery J., Ochoa R., van Engelsdorp D. and Bauchan G. R. 2018. A multi-microscopy approach to discover the feeding site and host tissue consumed by Varroa destructor on host honey bees. Microscopy and Microanalysis, 24 supplement s1.
Rangel J., Keller J. J. and Tarpy D. R. 2013 The effects of honey bee (Apis mellifera L.) queen reproductive potential on colony growth. Insectes Sociaux, 60: 65-67.
 Ruffinengo S., Eguaras M., Floris I., Faverin C., Bailac P.  and Ponzi M. 2005. LC50 and repellent effects of essential oils from Argentinian wild plant species on Varroa destructor. Journal of Economic Entomology, 98: 651-655.
Ruffinengo S., Maggi M., Faverin C., Rosa S. B., Bailac P., Principal J. and Eguaras M. 2007. Essential oils toxicity related to Varroa destructor and Apis mellifera under laboratory conditions. Zootecnia Tropical, 25: 63-69.
Sakofski F., Koeniger N. and Fuchs S. 1990. Seasonality of honey bee colony invasion by Varroa jacobsoni Oud. Apidologie, 21: 547-550.
Sebei K., Sakouhi F., Herchi W., Larbi Khouja M. and Boukhchina S. 2015. Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves. Biological Research, 48: 7.
Wade A., Lin C., Kurkul C., Ravasz Regan E. and Johnson R. M. 2019. Combined toxicity of insecticides and fungicides applied to California almond crchards to honey bee larvae and adults. Insects, 10: 1-11.
Williams G. R., Shafer A. B. A., Rogers R. E. L., Shutler D. and Stewart D. T. 2008. First detection of Nosema ceranae, a microsporidian parasite of European honey bees (Apis mellifera), in Canada and central USA. Journal of Invertebrate Pathology, 97: 189-192.
Yang Y. C., Choi H. Y., Choi W. S., Clark J. and Ahn Y. J. 2004. Ovicidal and adulticidal activity of Eucalyptus globulus leaf oil terpenoids against Pediculus humanus capitis (Anoplura: Pediculidae). Journal of Agricultural and Food Chemistry, 52: 2507-2511.
Animal Production Research
Volume 9, Issue 4
March 2021
Pages 1-10

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