Influence of vitamin D3 and lactic acid on performance, egg quality, and hatchability in broiler breeder hens

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Associate Professor, Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

3 Professor, Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

4 Associate Professor, Department of Poultry Sciences, College of Agricultural and Environmental Science, University of Georgia, Athens, USA

5 Assistant Professor, Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

Introduction: Vitamin D3 is one of the important vitamins in calcium metabolism, which is significantly involved in the absorption of calcium from the intestine. The main role of 1, 25-hydroxyvitamin D3 in vertebrates is to regulate calcium homeostasis, as 1, 25-hydroxyvitamin D3 has a direct effect on the gut, kidney, and bones by inhibiting the production of parathyroid hormone in the parathyroid glands. The synthesis of 1, 25-dihydroxycholecalciferol is tightly controlled, and the main stimulus for its synthesis is the reduction in plasma calcium. This is a feedback stimulus for the release of parathyroid hormone from the parathyroid gland. This hormone in turn stimulates the 1-hydroxylase enzyme complex in the kidney and causes the conversion of 25-hydroxycholecalciferol through the synthesis of calcium-binding protein in the duodenum, thereby increasing dietary calcium absorption and ultimately plasma calcium. Another powerful factor in calcium absorption is organic acids. By lowering the pH of the digestive tract, organic acids (lactic acid) prevent the formation of an insoluble complex of phytic acid with minerals, making phytate more sensitive to the action of endogenous phytase and preventing it from interfering with the absorption of minerals. Therefore, the purpose of this research was to study the effect of vitamin D3 and lactic acid on performance, egg quality, and hatchability in broiler breeders.
Materials and methods: A total of 240 broiler breeder hens and 24 roosters of Ross 308 strain were used in a completely randomized design with a 2×2 factorial arrangement including two levels of vitamin D (3500 and 5500 IU) and two levels of organic acid (zero and 500 mg/kg) in four treatments, six replications, and 10 hens and one rooster per replication. During the experiment, the percentage of hatchability, the number, and weight of eggs produced by each pen were recorded and using the common formulas of egg mass (as the percentage of laying multiplied by the average egg weight), the percentage of egg production as chicken-day and the feed conversion factor (as grams of feed consumed per gram of egg), the amount of feed used to produce each number of eggs and each piece of chicken, as well as the number of chickens produced per chicken was also calculated. Egg characteristics (egg weight and shape index) and egg shell quality (egg specific weight, eggshell weight compared to total egg weight and eggshell thickness), albumen and yolk pH, yolk index, Haugh unit, yolk color, percentage albumen and yolk percentage of four eggs from each replicate were measured every 28 days. The specific weight of the eggs was determined using the flotation method. The egg shape index was determined by measuring the width and length of the egg with a caliper to calculate the ratio of width to length. To determine eggshell parameters, eggs were identified and broken individually. Eggshells were washed under running water and dried at 35 °C for 72 hours. Then the eggshells were weighed and their relative weight was calculated. A digital micrometer (Series 500, Mitoyota, Tokyo, Japan) was used to measure the shell thickness.
Results and discussion: Results showed that the main effect of vitamin D3 at higher concentrations was affecting egg weight and reducing egg weight (P<0.05). The main effect of vitamin D3 at higher concentrations caused an increase in albumen pH, shell percentage, shell thickness, and specific gravity of the egg, and a decrease in yolk pH (P<0.05). Probably, with the increase in vitamin D3, the concentration of 1,25-cholecalciferol and the amount of calcium absorption increase, and since most of the shell is related to calcium carbonate, this leads to an increase in the thickness of the eggshell. The main effect of lactic acid showed a significant effect on performance parameters, and the addition of lactic acid improved performance (P<0.05). Lactic acid also significantly increased hatchability and shell thickness and reduced the number of broken eggs (P<0.05). It appears that organic acids increase the solubility of wheat phytates during germination. Therefore, acidification of the diet provides a better environment for phytase to reduce the amount of phytate present in digestion and flowing into the small intestine, thereby largely preventing the formation of insoluble mineral-phytate complexes and increasing the quality of the eggshell. Also, the main effect of lactic acid showed no significant influence on the internal quality of the eggs. The low levels of vitamin D3 and the addition of lactic acid improved shell thickness and reduced the percentage of shell breakage (P<0.05).
Conclusions: In general, according to the results of the present experiment, the use of lactic acid at the rate of 500 mg/kg along with 3500 IU of vitamin D3 can increase the percentage of production, shell thickness, reduce the number of broken eggs, improve the feed conversion ratio, increase chicken production and reduce feed consumption per egg and chicken at the end of the production period of broiler breeder hens.

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Abdulrahim S. M., Patel M. B. and McGinnis J. 1979. Effects of vitamin D3 and D3 metabolites on production parameters and hatchability of eggs. Poultry Science, 58: 858-863.
Afsharmanesh M. and Pourreza J. 2005. Effect of calcium, citric acid, ascorbic acid, vitamin D3 on the efficacy of microbial phytase in broiler starters fed wheatbased diets on performance, bone mineralization and ileal digestibility. International Journal of Poultry Science, 4(6): 418-424.
Atencio A., Edwards H. M. and Pesti G. M. 2005. Effect of the level of cholecalciferol supplementation of broiler breeder hen diets on the performance and bone abnormalities of the progeny fed diets containing various levels of calcium or 25-hydroxycholecalciferol. Poultry Science, 84: 1593-1603.
Bar A. and Hurwitz S. 1979. The interaction between calcium and gonadal hormones in their effect on plasma calcium, bone 25-hydroxycholecalciferol-1-hydroxylase, and duodenal calcium binding protein, measured by radioimmunoassay in chicks. Endocrinology, 104: 1455-1460.
Bar A., Striem S., Rosenburg J. and Hurwitz S. 1988. Eggshell quality and cholecalciferol metabolism in aged laying hens. Journal of Nutrition, 118: 1018-1023.
Browning L. C. and Cowieson A. J. 2015. Interactive effects of vitamin D3 and strontium on performance, nutrient retention, and bone mineral composition in laying hens. Journal of the Science of Food and Agriculture, 95: 1080-1087.
Castagliuolo I., Scarpa M. and Brun P. 2021. Co-administration of vitamin D3 and lacticaseibacillus paracasei DG increase 25-hydroxyvitamin D serum levels in mice. Annual Microbiology, 71(1): 42.
Coto S., Cerate Z., Wang F., Yan Y., Min F. P., Costa P. W. and Waldroup. 2010. Effect of source e level of vitamin D on the performance of breeder’s hens and carryover to the progeny. International Journal of Poultry Science, 7: 623-633.
Divari N., Kianfar R., Mirghelenj S. A. and Janmohammadi H. 2020. Effects of raw and autoclaved amaranth grain on performance and egg quality of layer hens. Research on Animal Production, 11(28): 32-40. (In Persian).
Duarte V., Minafra C. S., Santos F. R. D. and Perim F. D. S. 2015. Inclusion of canthaxanthin and 25- hydroxycholecalciferol in the diet of broiler breeders on performance and incubation parameters. Ciência Rural, 45: 2050-2055.
Dusso A. S., Brown A. J. and Slatopolsky E. 2005. Vitamine D. American Journal of Physiology, 289: F8-F28
Edmonds M. S., Lohalt S. and Moreland S. 2014. Effect of supplemental humic acid and butyric acid on performance and mortality in broilers raised under various environmental conditions. Journal of Applied Poultry Research, 23: 1-8.
Funk E. M. 1948. The relation of yolk index determined in natural position to the yolk index as determined after separating the yolk from the albumen. Poultry Science, 27: 367.
Garcia V. P., Catala-Gregori F., Hernandez-Megras M. and Madrid D. J. 2006. Effect of formic acid and plant extracts on growth, nutrient digestibility, intestine mucosa morphology, and meat yield of broilers. Journal Applied Poultry Resource, 16: 555-562.
Gasprovic B. C., Cabanas M. E., Alonso J., Murillo I., San Segundo B. and Arus C. 1997. Measurement of intracellular pH of maize seeds (Zea mays) during germination by P-nuclear magnetic resonance spectroscopy. Molecular and Cellular Biology, 43: 609-620.
Harms R. H., Bootwalla S. M., Woodward S. A., Wilson H. R. and Untawale G. A. 1990. Some observations on the influence of vitamin D metabolites when added to the diet of commercial laying hens. Poultry Science, 69: 426-432.
Holick M. F. 1981. The cutaneous photosynthesis of previtamin D3: A unique photoendocrine system. Journal of Investigation in Dermatolgy, 77: 51-58.
Hollander D., Muralidhara K. S. and Zimmerman A. 1978 Vitamin D3 intestinal absorption in vivo: influence of fatty acids, bile salts, and perfusate pH on absorption. Gut, 19: 267-272.
Iba A. M. and Berchieri-J.r. A. 1995. Studies on the use of a formic acid-propionic acid mixture (Bio-Add) to control experimental salmonella infection in broiler chickens. Avian Pathology, 24: 303-311.
Izat A. L., Tidwell N. M.., Thomas R. A., Reiber M. A., Adams M. H., Colberg M. and Waldroup P. W. 1990. Effects of a buffered propionic acid in diets on the performance of broiler chickens and on microflora of the intestine and carcass. Poultry Science, 69: 818-826.
KaetzeL. D. M., Soares J. H. and Swerdel M. R. 1978. Effects of vitamin D3 metabolites on the bones and eggshells of aged quail and chickens. Proceedings of the Maryland Nutrition Conference, College Park, MD. Pp. 50-54.
Kappeli S., Fröhlich E., Sabine G., Gebhardt-Henrich A., Pfulg Heidi Schäublin R., Zweifel H., Wiedmer and Stoffel M. H. 2011. Effects of dietary supplementation with synthetic vitamin D3 and 25 hydroxycholecalciferol on blood calcium and phosphate levels and performance in laying hens. Archiv für Geflügelkunde, 75: 179-184.
Kazemifard M., Nasiri-Moghadam H. and Saki A. A. 2010. Effect of different levels of calcium, phosphorus and vitamin D3 on the calcium, phosphorus and magnesium of plasma, hatchability and performance on broiler breeder hens. Resarch Journal of Biological Science, 5(2): 223-227.
Keshavarz K. 2003. A comparison between cholecalciferol and 25-OH-cholecalciferol on performance and eggshell quality of hens fed different levels of calcium and phosphorus. Poultry Science, 82: 1415-1422.
Klinsoda J., Vötterl J., Zebeli Q. and Metzler-zebeli B. U. 2019. Lactic acid treatment of cereals and dietary phytase modified fecal microbiome composition without affecting expression of virulence factor genes in growing pigs. Frontiers in Microbiolgy, 10: 2345.
Langhout P. 2000. New additives for broiler chickens. World Poultry, 16(3): 22-27.
Mattila P. H., Valkonen E. and Valaja J. 2011. Effect of different vitamin D supplementations in poultry feed on vitamin D content of eggs and chicken meat. Journal of Agricultural and Food Chemistry, 59: 8298-8303.
Mattila P., Valaja J., Rossow L., Venalainen E. and Tupasela T. 2004. Effect of vitamin D2- and D3-enriched diets on egg vitamin D content, production, and bird condition during an entire production period. Poultry Science, 83: 433-440.
Meng Q. W., Yan L., AO X., Jang H. D., Cho J. H. and Kim I. H. 2010. Effect chitooligosaccharide supplementation on egg production, nutrient digestibility, egg quality and blood profiles in laying hens. Journal of Applied Poultry Research, 18: 598-604.
Mirghelenj S., Kianfar R. Janmohammadi H. and Taghizadeh A. 2018. Effect of different levels of grape pomace on egg production performance and egg internal quality during different keeping times and temperatures. Animal Production Research, 6(4): 81-91. (In Persian).
Moghadam A. N., Pourreza J. and Samie A. H. 2006. Effect of different levels of citric acid on calcium and phosphorus efficiencies in broiler chicks. Pakistan Journal of Biological Sciences, 94(9): 1250-1256.
Nascimento D. O., Murakami G. R., Guerra A. Q. F. M., Rojas I. C., Ferreira M. F. Z. and Fanhani J. C. 2014. Effect of different vitamin D sources and calcium levels in the diet of layers in the second laying cycle. Revista Brasileira Deciencia Avícola, 16: 37-42.
Nys Y. 2001. Recent developments in layer nutrition for optimising shell quality. In: Proceedings of 13th European Symposium of Poultry Nutrition. Blankenberge, Belgium. Pp. 45-52.
Packard M. J. and Packard G. C. 1991. Patterns of mobilization of calcium, magnesium, and phosphorus by embryonic yellow-headed black birds (Xanthocephalus xanthocephalus). Journal of Comparative Physiology B, 160: 649-654.
Parks C. W., Grimes J. L., Ferket P. R. and Fairchild A. S. 2001. The effect of mannanoligosaccharides, mambermycins, and virginiamycin on performance of large white male market turkeys. Poultry Science, 80: 718-723.
Peng H. W., Ding X. M., Bai S. P., Luo Y. H., Zhu Q. and Zhang K. Y. 2013. Effects of maternal dietary 25- hydroxycholecalciferol and progeny dietary vitamin 370 premixes on progeny performance, immune responses and bone quality of broiler chicks. Journal of Food, Agriculture and Environment, 11: 701-706.
Pike J. W., Zella L. A., Meyer M. B., Fretz J. A. and Kim S. 2007. Molecular actions of 1, 25‐dihydroxyvitamin D3 on genes involved in calcium homeostasis. Journal of Bone and Mineral Research, 22(S2): V16-V19.
Plaimast H. and Kijparkorn S. 2010. Effects of supplementary vitamin D3 on eggshell quality and vitamin D3 content in egg of aged hens fed different levels of calcium. Proceedings of the 9th Chulalongkorn University Veterinary. Annual Conference.
Plaimast H., Kijparkorn S. and Ittitanawong P. 2015. Effects of vitamin D3 and calcium on productive performance, egg quality and vitamin D3 content in egg of second production cycle hens. Thai Journal of Veterinary Medicine, 45: 189-195.
Rafacz-Livingston K. A., Amezcua C. M., Parsons C. M., Baker D. H. and Snow J. 2005. Citric acid improves phytate phosphorus utilization in crossbred and commercial broiler chicks. Poultry Science, 84: 1370-1375.
Roy R. D., Edens F. W., Parkhurst C. R., Qureshi M. A. and Havenstein G. B. 2002. Influence of a propionic acid feed additive on performance of turkey poults with experimentally induced poult enteritis and mortality syndrome. Poultry Science, 81: 951-957.
Salvador D., Faria D. E. D., mazalli M. R., Ito D. T., Faria Filho D. E. and Araújo L. F. 2009. Vitamins D and C for laying hens at the initial phase of egg production. Revista Brasileira Dezootecnia, 38: 887-892.
Saunders-blades J. L. and Korver D. R. 2014. The effect of maternal vitamin D source on broiler hatching egg quality, hatchability, and progeny bone mineral density and performance. The Journal of Applied Poultry Research, 23: 773-783.
Scott M. L., Nesheim M. and Young R. 1982. Nutrition of the chicken, Scott, Ithaca, Nueva York. P.119.
Soares J. H., Ottinger M. A. and Buss E. G. 1988. Potential role of 1, 25-dihydroxycholecalciferol in egg shell calcification. Poultry Science, 67: 1322-1328.
Soares J. H., Swerdel M. R. and Ottinger M. A. 1979. The effectiveness of the vitamin D analog 1 α-OH-D3 in promoting fertility and hatchability in the laying hen. Poultry Science 58: 1004-1006.
Soltan M. A. 2008. Effect of dietary organic acid supplementation on egg production, egg quality and some blood serum parameters in laying hens. International Journal of Poultry Science, 7: 613-621.
Torres C. A., Vieira S. L., Reis R. N., Ferreira A. K., Silva P. X. D. and Furtado F. V. F. 2009. Productive performance of broiler breeder hens fed 25-415 hydroxycholecalciferol. Revista Brasileira Dezootecnia, 38: 1286-1290.
Valizadeh R., Kianfar R. Mirghelenj S. and Olyaee M. 2018. Interaction effect of ginger root and red pepper powders in wheat based diet on performance and immune response of layer hens in post-molting period. Animal Production Research, 7(1), 81-92. (In Persian).
Vötterl J. C., Klinsoda J., Zebeli Q., Hennig-Pauka I., Kandler W. and Metzler-Zebeli B. U. 2020. Dietary phytase and lactic acid-treated cereal grains differently affected calcium and phosphorus homeostasis from intestinal uptake to systemic metabolism in a pig Model. Nutrients, 12: 1542.
Washburn K. W. 1982. Incidence, cause, and prevention of eggshell breakage in commercial production. Poultry Science, 61: 205-2012.
Yalcin S. K., Bozdemir M. T. and Ozbas Z. Y. 2009. A comparative study on citric acid production kinetics of two Yarrowia lipolytica strains in two different media. Indian Journal of Biotechnology, 8: 408-417.
Yesilbag D. and Colpan I. 2006. Effects of organic acid supplemented diets on growth performance, egg production and quality and on serum parameters in laying hens. Revue de Medecine Veterinaire, 157(5): 280-284.
Zhong Y., Armbrecht H. J. and Christakos S. 2009. Calcitonin, a regulator of the 25-hydroxyvitamin D3 1α-hydroxylase gene. Journal of Biological Chemistry, 284(17): 11059-11069.