Effect of unprotected methionine and choline on feed intake, growth, digestibility, fecal score, blood metabolites, and feeding behavior of suckling calves

Document Type : Research Paper

Authors

1 Professor, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

2 Former Ph.D. Student, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

3 Assistant Professor, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

Abstract

Introduction: Choline and methionine are crucial nutrients for livestock as they provide methyl groups. The relationship between these two nutrients is intricate, with varying opinions on their interdependence. Methionine is often a limiting amino acid in dairy diets and its metabolism is linked to that of choline and betaine. Choline as an alternative to methionine, can act as a precursor to phosphatidylcholine, which is important for dairy cows around calving time. Furthermore, choline and betaine impact methionine requirements and methionine can affect choline metabolism. Given that limited study and experimentation have been conducted so far to investigate the effects of unprotected methionine and choline on calves, this research aimed to examine the impact of unprotected methionine and choline on feed intake, growth, digestibility, fecal score, blood metabolites, and feed behavior of pre-weaned calves. The expected hypothesis of the study was the significant effect of utilizing unprotected methionine and choline on feed intake, growth, digestibility, fecal score, blood metabolites, and feeding behavior of pre-weaned calves due to their mono-gastric nature and lack of rumen development.
Materials and methods: A total number of 32 Simmental male suckling calves, aged approximately one month old with an initial weight of 44±2.1 kg, were used in this study. The experimental treatments included: 1) a basal diet without choline and methionine (control), 2) the basal diet with three grams of methionine, 3) the basal diet with three grams of choline, and 4) the basal diet with 1.5 grams of methionine and 1.5 grams of choline per head per day. The calves were randomly assigned to the experimental treatments in a completely randomized design with eight replications. The study lasted for 45 days, including a 7-day adaptation period. Blood glucose, cholesterol, triglycerides, and urea nitrogen levels were measured on the last day of the study, four hours after morning feeding, to evaluate the metabolic profile of the animals. Additionally, feed consumption behavior was visually observed for 24 hours on the last day of the study to calculate the animal's rumination, eating, chewing, and resting time. To determine the digestibility of dry matter, samples were collected from feed and feces during the last three days of the experiment using the acid-insoluble ash internal indicator method. Stool consistency was scored for all of the calves as an indicator of animal health.
Results and discussion: The inclusion of three grams of methionine in the experimental treatments resulted in a decrease in dry matter intake, and when methionine and choline were added, it increased the daily weight gain of the animals (P<0.05). However, the treatments did not have a significant effect on the feed conversion ratio (P>0.05). The results of other studies showed that the addition of choline did not have a significant effect on the amount of dry matter consumed, daily weight gain, and feed conversion ratio of Holstein bull calves. Consistent with the results of the present study, no improvement was observed in the feed efficiency of steers with the addition of a protected choline supplement, and no effect was observed on dry matter intake. The treatments did not have a significant impact on dry matter digestibility, stool consistency, blood glucose, cholesterol, triglyceride, and urea nitrogen (P>0.05). In agreement with the results of the present study, the digestibility of dry matter and organic matter in steers was not affected by choline. Researchers reported that the use of protected biocholine and methionine did not have a significant effect on the digestibility of dry matter in calves. Also, it was observed that the use of choline did not have a significant effect on the digestibility of dry matter in sheep and pre-weaned calves. Researchers have reported that the use of protected methionine and choline did not have a significant effect on the levels of glucose and triglycerides in the blood of dairy cows before calving. In another study, the urea nitrogen in the blood of dairy cows was not affected by methionine and choline. Additionally, the experimental treatments did not affect feed consumption behavior (P>0.05).
Conclusions: Feeding unprotected choline and methionine did not have a significant effect on glucose, cholesterol, triglycerides, blood urea nitrogen, digestibility of dry matter, fecal consistency score, and feeding behavior. However, the use of methionine improved dry matter intake and daily weight gain. Feeding Simmental calves with three grams of methionine per day is recommended to improve dry matter intake and daily weight gain, although it does not significantly affect the feed conversion ratio.

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Amrutkar, S. A., Pawar, S. P., Thakur, S. S., Kewalramani, N. J., & Mahesh, M. S. (2015). Dietary supplementation of rumen-protected methionine, lysine and choline improves lactation performance and blood metabolic profile of Karan-Fries cows. Agricultural Research, 4(4), 396-404. doi: 10.1007/s40003-015-0178-2
Araujo, R. C., Pires, A. V., Susin, I., Mendes, C. Q., Rodrigues, G. H., Packer, I. U., & Eastridge, M. L. (2008). Milk yield, milk composition, eating behavior, and lamb performance of ewes fed diets containing soybeen hulls replacing coastcross (Cynodon species) hay. Journal of Animal Science, 86, 3511-3521. doi: 10.2527/jas.2008-0940
Ardalan, M., Dehghan-Banadaky, M., Rezayazdi, K., and Ghavi Hossein-Zadeh, N. (2011). The effect of rumen-protected methionine and choline on plasma metabolites of Holstein cows. Journal of Agricultural Science, 149, 639-646. doi: 10.1017/S0021859610001292
Association of Official Analytical (AOAC.) (1999). Official Methods of Analysis. Washington, D. C., USA.
Atkins, K. B., Erdman, R. A., & Vandersall, J. H. 1988. Dietary choline effects on milk yield and duodenal choline flow in dairy cattle. Journal of Animal Science, 71, 109-116.  doi: 10.3168/jds.S0022-0302(88)79531-4
Bindel, D. J., Drouillard, J. S., Titgemeyer, E. C., Wessels, R. H., & Loest, C. A. (2000). Effects of ruminally protected choline and dietary fat on performance and blood metabolites of finishing heifers. Journal of Animal Science, 78, 2497-2503. doi: 10.2527/2000.78102497x
Bindel, D. J., Titgemeyer, E. C., Drouillard, J. S., & Ives, S. E. (2005). Effects of choline on blood metabolites associated with lipid metabolism and digestion by steers fed corn-based diets. Journal of Animal Science, 83, 1625-1632.  doi: 10.2527/2005.8371625x
Bryant, T. C., Rivera, J. D., Galyean, M. L., Duff, G. C., Hallford, D. M., & Montgomery, T. H. 1999. Effects of dietary level of ruminally protected choline on performance and carcass characteristics of finishing beef steers and on growth and serum metabolites in lambs. Journal of Animal Science, 77, 2893-2903. doi: 10.2527/2005.8371625x
Chung, Y. H., Brown, N. E., Martinez, C. M., Cassidy, T. W., & Varga, G. A. (2009). Effects of rumen-protected choline and dry propylene glycol on feed intake and blood parameters for Holstein dairy cows in early lactation. Journal of Dairy Science, 92, 2729-2736. doi: 10.3168/jds.2008-1299
Daniyalzadeh, A., & Zareian, Kh. (1995). Principles of Biochemistry. Tehran University Jihad Publications. [In Persian]
Davidson, S., Hopkins, B. A., Odle, J., Brownie, C., Fellner, V., & Whitlow, L. W. (2008). Supplementing limited methionine diets with rumen-protected methionine, betaine, and choline in early lactation Holstein cows. Journal of Dairy Science, 91, 1552-1559. doi: 10.3168/jds.2007-0721
Drouillard, J. S., Flake, A. S., & Kuhl, G. L. (1998). Effects of added fat, degradable intake protein, and ruminally protected choline in diets of finishing steers. K-State's Cattlemen's Day Program, 804, 75. doi: 10.4148/2378-5977.1910
Erdman, R. A., & Sharma, B. K. (1991). Effect of dietary rumen-protected choline in lactating dairy cows. Journal of Dairy Science, 74, 1641-1647. doi: 10.3168/jds.S0022-0302(91)78326-4
Galyean, M. L., Harris, S. C., Nunnery, G. A., Salyer, G. B., Defoor, P. J., Robinson, K. D., & Rocha, R. I. (1999). Effects of source and level of ruminally protected choline on performance and carcass characteristics of finishing beef steers. research report. Department of Animal Sciences and food Tech., Texas Tech. University, Lubbock, 79409-21480.
Grant, R. J., Colenbrander, V. F., & Mertens, D. R. (1990). Milk fat depression in dairy cows: role of particle size of alfalfa hay. Journal of Dairy Science, 73, 1823-1833. doi: 10.3168/jds.S0022-0302(90)78862-5
Hajilou, M., Dehghan-Banadaky, M., Zali, A., & Rezayazdi, K. (2015). Effect of choline and L-carnitine on performance, apparent digestibility and carcass characteristics of Holstein male calves. Journal of Animal Science Research, 25(4), 11-19. [In Persian]
Hartwell, J. R., Cecava, M. J., & Donkin, S. S. (2000). Impact of dietary rumen- undegradable protein and rumen-protected choline on intake, peripartum liver triacylglyceride, plasma metabolites and milk production in transition dairy cows. Journal of Dairy Science, 83, 2907-2917. doi: 10.3168/jds.S0022-0302(00)75191-5
Hosseinabadi, M., Torbatinejad, N. M., Ghoorchi, T., & Toghdory, A. (2022). Effect of flaxseed  level and processing method of performance, skelet growth indices, health, and rumination behavior of suckling calves. Animal Production Research, 11(2), 31-42. doi: 10.22124/ar.2022.19593.1617. [In Persian]
Janovick Guretzky N. A., Carlson D. B., Garrett J. E., & Drackley J. K. (2006). Lipid metabolite profiles and milk production for Holstein and Jersey cows fed rumen-protected choline during the periparturient period. Journal of Dairy Science, 89, 188-200. doi: 10.3168/jds.S0022-0302(06)72083-5
Krober, T. F., Kreuzer, M., Senn, M., Langhans, W., & Sutter, F. (2000). Lactational and metabolic effects in cows of lysine and methionine added to a ration deficient according to the I.N.R.A. method. Archives of Animal Nutrition, 53, 375-394. doi: 10.1080/17450390009381959
Larson, L. L., Owen, F. G., Albright, J. L., Appleman, R. D., Lamb, R. C., & Muller, L. D. (1977). Guidelines toward more uniformity in measuring and reporting calf experimental data. Journal of Dairy Science, 60, 6. doi: 10.3168/jds.S0022-0302(77)83975-1
Li, H., Wang, H., Yu L., Wang, M., Liu, S., Sun, L., & Chen, Q. (2015). Effects of supplementation of rumen-protected choline on growth performance, meat quality and gene expression in longissimus dorsi muscle of lambs. Archives of Animal Nutrition, 69, 340-350. doi: 10.1080/1745039X.2015.1073001
Maekawa M., Beauchemin K. A., & Christensen, D. A. (2002). Chewing activity, saliva production, and ruminal pH of primiparous and multiparous Lactating Dairy Cows. Journal of Dairy Science, 85, 1176-1182. doi: 10.3168/jds.S0022-0302(02)74180-5
Mertens, D. R. (1997). Creating a system for meeting the fiber requirements of dairy Cow. Journal of Dairy Science, 80, 1463-1481. doi: 10.3168/jds.S0022-0302(97)76075-2
Moura, L. V., Oliveira, E. R., & Fernandes, A. R. M. (2017). Feed efficiency and carcass traits of feedlot lambs supplemented either monensin or increasing doses of copaiba (Copaifera spp.) essential oil. Journal of Animal Feed Science and Technology, 232, 110-118. doi: 10.1016/j.anifeedsci.2017.08.006
National Research Council (NRC). (2001). Nutrient Requirements of Dairy Cattle. 7th revised edition. National Academy of Science, Washington, DC.
Piepenbrink, M. S., & Overton, T. R. (2003). Liver metabolismand production of cows fed increasing amounts of rumen-protected choline during the periparturient period. Journal of Dairy Science, 86, 1722-1733. doi: 10.3168/jds.S0022-0302(03)73758-8
Pinotti, L., Baldi, A. M., & Dell’Orto, V. (2002). Comparative mammalian choline metabolism with emphasis on thehigh-yielding dairy cow. Nutrition Research Review, 15, 315-331. doi: 10.1079/NRR200247  
Pinotti, L., Baldi, A., Politis, I., Rebucci, R., Sangalli, L., & Dell’Orto, V. (2003). Rumen-protected choline administration to transition cows: effects on milk production and vitamin E ststus. Journal of Veterinary Medicine Series A, 50, 18-21. doi: 10.1046/j.1439-0442.2003.00502.x
Pipenbrink, M. S., Overton, T. R., & Clark, J. H. (1996). Response of cows fed a low crude protein diet to ruminally protected methionine and lysine. Journal of Dairy Science, 79, 1638-1646. doi: 10.3168/jds.S0022-0302(96)76527-X
Powell, J. M., Broderick, G. A., & Misselbrook, T. H. (2008). Seasonal diet affects ammonia emissions from tiestall dairy barns. Journal of Dairy Science, 91, 857-869. doi: 10.3168/jds.2007-0588
Rodríguez-Guerrero, V., Lizarazo, A. C., Ferraro, S., Suárez, N., Miranda, L. A., & Mendoza, G. D. (2018). Effect of herbal choline and rumen-protected methionine on lamb performance and blood metabolites. South African Journal of Animal Science, 48(3), 427-434. doi: 10.4314/sajas.v48i3.3
Sales, J., Homolka, P., & Koukolová, V. (2010). Effect of dietary rumen-protected choline on milk production of dairy cows: A meta-analysis. Journal of Dairy Science, 93, 3746-3754. doi: 10.3168/jds.2010-3106
SAS. (2003). Statistical Analysis System user’s guide. SAS 9.1. Cary (NC): SAS Institute.
Sharma, B. K., & Erdman, R. A. (1988). A. Abomasal infusion of choline and methionine with or without 2-amino-2-methyl-1-propanol for lactating dairy cows. Journal of Dairy Science, 71, 2406-2411. doi: 10.3168/jds.S0022-0302(88)79825-2
Soltan, M. A., Mujalli, A. M., Mandour, M. A., & Abeer, M. E. (2012). Effect of dietary rumen protected methionine and/or choline supplementation of rumen fermentation characteristics and protective performance of early lactating cows. Pakistan Journal of Nutrition, 11(3), 221-230. doi: 10.3923/pjn.2012.221.230
Suksombat, W., Homkao, J., & Klangnork, P. (2012). Effect of biotin and rumen protected choline supplementation on milk production, milk composition, live weight change and blood parameters in lactating dairy weights. Journal of Animal and Veterinary Advances, 10, 2186-2192. doi: 10.3923/javaa.2012.1116.1122
Sun, F., Cao, Y., Cai, C., Li, S., Yu, C., & Yao, J. (2016). Regulation of nutritional metabolism in transition dairy cows: Energy homeostasis and health in response to post-ruminal choline and methionine. PloS One, 11, 8, e0160659. doi: 10.1371/journal.pone.0160659
Thomas, L. C., Wright, T. C., Formusiak, A., Cant, J. P., & Osborne, V. R. (2007). Use of flavored drinking water in calves and lactating dairy cattle. Journal of Dairy Science, 90, 3831-3837. doi: 10.3168/jds.2007-0085
Toghdory, A., Ghoorchi, T., Naserian, A., Jafari Ahangari, Y., & Hassani, S. (2009). Nutritional effects of choline on productive performance and some blood metabolites of lactating Holstein dairy cow. Journal of Agricultural Sciences and Natural Resources, 16(1). [In Persian]
Van Keulen, J., & Young, B. A. (1977). Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44, 282-287. doi: 10.2527/jas1977.442282x
Van Soest, P. J. (1994). Nutritional Ecology of the Ruminants. Cornell University Press, Ithaca, New York. steers. Nebraska Beef Cattle Reports, 67A, 72-74.
Yao, Z. M., & Vance, D. E. (1988). The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes. Journal of Biological Chemistry, 263, 2998-3004.
Ye, J. A., Wang, C., Wang, H. F., Liu, H. Y., Wang, Y. M., & Chen, B. (2010). Effects of pelletizing and supplementary methionine, lysine, and choline on the performance of periparturient dairy cows. Acta Agriculturae Scandinavica, 60(10), 230-238. doi: 10.1080/09064702.2010.532566
Zahra, L. C., Duffield, T. F., Leslie, K. E., Overton, T. R., Putnam, D., & Leblank, S. J. (2006). Effects of rumen-protected choline and monensin on milk production and metabolism of periparturient dairy cows. Journal of Dairy Science, 89, 4808-4818. doi: 10.3168/jds.S0022-0302(06)72530-9
Zeisel, S. H. (2006). Choline: critical role during fetal development and dietary requirements in adults. Annual Review of Nutrition, 2(1), 229-250. doi: 10.1146/annurev.nutr.26.061505.111156
Zeisel, S. H., & da Costa, K. (2009). Choline: An essential nutrient for public health. Nutrition Reviews, 67, 615-623. doi: 10.1111/j.1753-4887.2009.00246.x
Zhou, Z., Vailati-Riboni, M., Trevisi, E., Drackley, J. K., Luchini, D. N., & Loor, J. J. (2016). Better postpartal performance in dairy cows supplemented with rumen protected methionine compared with choline during the peripartal period. Journal of Dairy Science, 99, 1-17. doi: 10.3168/jds.2015-10525