Effect of milk fortified with natural honey on performance, digestibility, blood metabolites, and skeletal growth indices of suckling Holstein calves

Document Type : Research Paper

Authors

1 MSc Student, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Professor, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Assistant Professor, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 Ph.D. Student, Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Introduction: The management of suckling calves guarantees health and performance in their productive life. The sensitivity and low immune system in newborn calves increase the odds ratio of some disorder incidence and sometimes it is associated with the death. Therefore, the use of antibiotics in suckling calves became popular. Laws prohibiting the use of antibiotics in raising domestic animals led to the use of natural alternatives with similar properties. These compounds cause positive effects on the reduction of intestinal infections, and disorders and increase the absorption of nutrients by creating intestinal microbial balance. Prebiotics have the same feature that changes the microflora population in the digestive system. It has been proven honey has prebiotic characteristics that improve the immune system in mammals. This product and its by-products are used for different goals in human feed. This prevents intestinal infection, modifies intestine microflora population, and improves health situation and lipid metabolism. Honey is a natural product that is thickened from saturated or supersaturated sugar solutions. It usually consists of 17% water, 38% fructose, 31% glucose, 10% other sugars, and a wide range of micronutrients, vitamins, minerals, and amino acids, with a pH below 4. This study was conducted to evaluate the effect of milk enrichment with natural honey on the performance, digestibility, blood parameters, and skeletal growth indices of suckling Holstein calves.
Materials and methods: Eighteen Holstein suckling male calves with an average weight of 58±4.2 kg were used. Treatments were divided into three groups which contained zero, 2.5, and five g per day of natural honey to the milk consumed. The duration of the experiment was 30 days evaluated in a completely randomized design. Calves were kept in individual pens and fed based on NRC requirements. Starter provided to total mixed ration (TMR) form which is given at 8:00 am and 4:00 pm daily. Calves fed milk based on 10% of body weight in the morning and evening. The amount of milk consumed by calves is measured, and to calculate the milk solids, it is multiplied by a coefficient of 12.5%. Dry matter (DM) digestibility, blood samples, average daily gain, dry matter intake, stool score, and skeleton growth were evaluated during 30 d and compared treatment by the general mixed model in SAS software.
Results and discussion: The results of this study showed that adding natural honey to milk significantly improved the feed conversion ratio and increased the final weight, average daily gain, and dry matter digestibility of the calves (P<0.05). The group of calves that consumed five g of natural honey per day had the highest final weight and average daily gain, as well as the lowest feed conversion ratio. However, the milk and starter intakes were not affected by the experimental treatments, and there was no significant difference observed between the control group and the groups that received natural honey (P<0.05). Supplementing milk with honey improved the growth rate and feed conversion ratio of the calves, likely due to the presence of enzymes and other substances in honey that aid in breaking down polysaccharides into usable energy for the animals. Blood glucose concentration decreased in the calves that received natural honey (P<0.05), but the concentrations of cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were not significantly different among the treatments (P>0.05). The reduction in blood glucose can be attributed to the antioxidant compounds present in honey, which can reduce intestinal glucose absorption by inhibiting the digestive enzymes of alpha-amylase or delaying the emptying of stomach contents into the small intestine. The stool consistency score increased with increasing levels of natural honey in the milk, and the group that received five g of natural honey per day had the best stool score, while the control group had the lowest stool consistency score (P<0.05). However, there was no effect of treatment on the number of animals with diarrhea or the number of days involved with diarrhea (P>0.05). Natural honey is rich in antioxidants and has prebiotic properties, which promote the growth of beneficial microorganisms and decrease harmful microflora in the gut. There was no significant difference observed in skeletal growth indices between the different experimental treatments (P>0.05).
Conclusions: The experiment's findings indicated that incorporating natural honey (up to five g/day) into the milk given to Holstein calves can improve their performance and health, making it a recommended practice.

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Abuharfeil, N., Al-Oran, R., & AboShehawa, M. (1999). The effect of bee honey on the proliferation activities of human B-and Tlymphocytes and activity of phagocytes. Food and Agricultural Immunology, 11, 169-77.
Adebolu, T. T. 2005. Effect of natural honey on local isolates of diarrhea causing bacteria in South Western Nigeria. African Journal of Biotechnology, 4, 1172-1174.
Ajibola, A., Olusakin, J., & Oyewale, A. (2016). Growth and metabolic response of suckling rats fed with natural honey supplements. International Journal of Food Sciences and Nutrition, 3(1), 199-203. doi.org/10.15436/2377-0619.16.041
AOAC. (2005). Official Method of Analysis. 18th Edition, Association of Officiating Analytical Chemists, Washington DC, Method 935.14 and 992.24.
Babaei, C., Rahimi, S., Karimi, Torshizi, M. A., Tahmasabi, G., & Khaleghimiran, N. (2016). Effect of honey, royal jelly and bee pollen on performance, safety system and blood parameters Japanese quail. Animal Production, 17(2), 311-320. doi: 10.22059/jap.2015.53826 [In Persian]
Busserolles, J., Gueux, E., Rock, E., Mazur, A., & Rayssiguier, A. (2002). Substituting honey for refined carbohydrates protects rats from hyper triglycerdemic and pro oxidative effect on fructose. Journal of Nutrition, 132, 3379-3382. doi: 10.1093/jn/132.11.3379
Camacho-Bernal, G., del Socorro, I., Cruz-Cansino, N., Ramírez-Moreno, E., Delgado-Olivares, L., Zafra-Rojas, Q. Y., Castañeda-Ovando, A., & Suárez-Jacob, Á. (2021). Addition of bee products in diverse food sources: functional and physicochemical properties. Applied Sciences, 11, 8156-8171. doi: 10.3390/app11178156
Campos, A. M. A., Rostagno, H. S., & Gomes, P. C. (2011). Effect of in ovo inoculation of nutritious solutions on the hatchability and performance of broiler chickens. Revista Brasileira de Zootecnia, 40, 1712-1717. doi: 10.33448/rsd-v9i8.5178
Dal’Alba, G. M., Melek, C., Schneider, M., Deolindo, G. L., Boiago, M. M., Faria, G. A., Stefani, L. M., da Silva, A. S., & Araujo, D. N. (2020). In vivo nutrition using honey: effects on hatchability, performance and carcass yields in broilers. Research, Society and Development, 9(8), 1-17. doi: 10.33448/rsd-v9i8.5178
Ekanem, J. T., Majolagbe, O. R., Suileman, F. A., & Muhammad, N. O. (2006). Effect of honeys up plemented diet on the parasitemia and some enzymes of trypanosome brucei-infected rats. African Journal of Biotechnology, 5(17), 1557-1561.
El-Hanoun, A., Elkomy, A. E., El-Sabrout, K., & Abdella, M. (2020). Effect of bee venom on reproductive performance and immune response of male rabbits. Physiology and Behavior, 223, 112987. doi: 10.1016/j.physbeh.
Erejuwa, O., Sulaiman, S., Wahab, M., Sirajudeen, K., Salleh, M., & Gurtu, S. 2012. Hepatoprotective effect of tualang honey supplementation in streptozotocin-induced diabetic rats, International Journal of Applied Research in Natural Products, 4(4), 37-41.
Gheldof, N., Wang, X. H., & Engeseth, N. J. (2002). Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricultural and Food Chemistry, 50, 5870-5877. doi: 10.1021/jf0256135
Ghosh, S., Mehla, R. K., Sirohi, S. K., & Tomar, S. K. (2011). Performance of crossbred calves with dietary supplementation of garlic extract. Journal of Animal Physiology and Animal Nutrition, 95, 449-455. doi: 10.1111/j.1439-0396.2010.01071.x
Heinrichs, A. J., Jones, C. M., Elizondo-Salazar, J. A., & Terrill, S. J. ( 2009). Effects of a prebiotic supplement on health of neonatal dairy calves. Livestock Science, 125, 149-154. doi: 10.1016/j.livsci.2009.04.003
Hosseinabadi, M., Torbatinejad, N. M., Ghoorchi, T. & Toghdory, A. (2022). Effect of flaxseed level and processing method of performance, skelet growth indices, health, and rumination behavior of suckling calves. Animal Production Research, 11(2), 31-42. 10.22124/ar.2022.19593.1617 [In Persian]
Isa, A., Aliyu A., Daniel, E., Lawal, A., Dewu, M., & Muhammad, M. (2013). Evaluation of the hypoglycaemic effect of honey in alloxan-induced diabetic wistar rats. Scientific Journal of Pure and Applied Sciences, 2(5), 224-230. doi: 10.14196/sjpas.v2i5.625
Jatkauskas, J., & Vrotniakiene, V. (2010). Effects of probiotic dietary supplementation on diarrhoea patterns, faecal microbiota and performance of early weaned calves. Veterinarni Medicina, 55(10), 494-503. doi: 10.17221/2939-VETMED
Khan, M. A., Lee, H. J., Lee, W. S., Kim, H. S., Kim, S. B., Ki, K. S., Ha, J. K., Lee, H. G., & Choi, Y. J. (2007). Pre- and post-weaning performance of Holstein female calves fed milk through step-down and conventional methods. Journal of Dairy Science, 90, 876–885. doi: 10.3168/jds.S0022-0302(07)71571-0
Khan, M. A., Weary, D. M., & Keyserlingk, M. A. (2011). Invited review: Effects of milk ration on solid feed intake, weaning, and performance in dairy heifers. Journal of Dairy Science, 94, 1071-1081. doi: 10.3168/jds.2010-3733
Kim, D. H., Han, S. M., Yun-Sang, C., Kang, H. K., Hong-Gu, L., & Kyung woo, L. (2019). Effects of dietary bee venom on serum characteristic, antioxidant activity and liver fatty acid composition in broiler chickens. Korean Journal of Poultry Science, 46, 39-46. doi: 10.5536/KJPS.2019.46.1.39
Laila, R., Parveen, F., Khan, M., Rahman, H., Ara, F., & Saha, R. (2012). Effect of natural honey on bloodglucose level of alloxan induced diabetic rats, Journal of Dhaka Medical College, 20(2),119-123. doi: 10.3329/jdmc.v20i2.10537
Langford, R. M., Weary, D. M., & Fisher, L. (2003). Antibiotic resistance in gut bacteria from dairy calves: a dose response to the level of antibiotics fed in milk. Journal of Dairy Science, 86, 3963-3966. doi: 10.3168/jds.S0022-0302(03)74006-5
Lesmeister, K., Heinrichs, A., & Gabler, M. (2004). Effects of supplemental yeast Saccharomyces cerevisiae culture on rumen development, growth characteristics and blood parameters in neonatal dairy calves. Journal of Dairy Science, 87, 1832-1839. doi: 10.3168/jds.S0022-0302(04)73340-8
Lukacinova, A., Mojzis, J., Benacka, R., Keller, J., Maguth, T., Kurila, P., Vasko, L., Racz, O. & Nistiar, F. 2008. Preventive effects of flavonoids on alloxan-induced diabetes mellitus in rats. Acta Veterinaria Brno, 77, 175-182. doi: 10.2754/avb200877020175
Mandal, A. B., Yadav, A. S., Johri, T. S., & Pathak, N. N. (2004). Nutrition and disease management of poultry. International Book distribution CO. Lucknow, India, Pp. 12-370.
Mohammadimanesh, A., Khosravi, H. M., Vahidiniya, A. A., Doaei, S., Salehi, I., & Fayyaz, N. (2016). The comparative effect of different types of honey on levels of glucose, fructosamine and insulin in Streptozocin-induced diabetes in wistar rats. South Asian Journal of Experimental Biology, 6(1), 39-44.‏
Moarrab, A., Ghoorchi, T., Ramezanpour, S., Ganji, F., & Koochakzadeh, A. R. (2016). Effect of synbiotic on performance, intestinal morphology, fecal microbial population and blood medtabolites of suckling lambs. Iranian Journal of Applied Animal Science, 6(3), 621-628.
Munstedt, K., Bohme, M., & Hauenschild, A. (2011). Consumption of rapeseed honey leads to higher serum fructose levels compared with analogue glucose / fructose solutions. European Journal of Clinical Nutrition, 65, 77-80. doi: 10.1038/ejcn.2010.186
Nguyen, H. T. L., Panyoyai, N., Kasapis, S., Pang, E., & Mantri, N. (2019). Honey and its role in relieving multiple facets of atherosclerosis. Nutrients, 11(1), 167. doi: 10.3390/nu11010167
NRC. (2001). Nutrient requirements of dairy cows. 7th edition. National Academy of Sciences, Washington D. C.
Obun, C. O., Yahayab, M. S., Olafadehanc, O. A., Kehindea, A. S., Adeyemid, O. A., Farouka, I. U., & Allison, D. S. (2010). Effect of honey-flavoured diets on the performance and relative organ weights of finisher broiler chickens. Nigerian Society for Animal Production, 38(1), 64-72.‏ doi: 10.51791/njap.v38i1.698
Rodriguez, C., Castro, N., Capote, J., Morales-delanuez, A., Moreno-Indias, H., Sanchez-Macias, D., & Arguello, A. 2009. Effect of colostrum immunoglobulin concentration on immunity in Majorera goat kid. Journal of Dairy Science, 92, 1696-1701. doi: 10.3168/jds.2008-1586
Sarfraz, A., Amrah Sulaiman, S., Amin Baig, A., Ibrahim, M., Liaqat, S., Fatima, S., Jabeen, S., Shamim, N., & Othman, N. (2018). Honey as a potential natural antioxidant medicine: an insight into its molecular mechanisms of action. Oxidative Medicine and Cellular Longevity, 2018, 8367846. doi: 10.1155/2018/8367846
Sarker, M. S. K., & Yang, C. J. (2010). Propolis and illite as feed additives on performance and blood profiles of post-weaning Hanwoo calves. Journal of Animal and Veterinary Advances, 9, 2704-2709. doi: 10.3923/javaa.2010.2526.2531
SAS Institute. (2004). User’s Guide. Version 9.1: Statistics. SAS Institute, Cary, NC.
Schiffrin, E. J., & Blum, S. (2002). Interactions between the microbial and the intestinal mucosa. European Journal of Clinical Nutrition, 56(3), 60-64. doi: 10.1038/sj.ejcn.1601489
Shamala, T. R., Shrijyoth, Y., & Saibaba, P. (2000). Stimulatory effect of honey on multiplication of lactic acid bacteria under in vitro and in vivo conditions. Letters in Applied Microbiology, 30, 453-455. doi: 10.1046/j.1472-765x.2000.00746.x
Yakhkeshi, S., Rahimi, S., & Gharib Naseri, K. (2011). The effects of comparison of herbal extracts, antibiotic, probioticand organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers. Journal of Medicinal Plants, 37, 80-95. doi: 20.1001.1.2717204.2011.10.37.10.1
Van Keulen, J. B., & Young, A. (1977). Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44(2), 282-287. doi: 10.2527/jas1977.442282x
Zhu, R., Hesong, L. V., Tianye L., Yunqiang, Y., Jianing, W., & Shaoze, Y. (2016). Feeding kinematics and nectar intake of the honey bee tongue. Journal of Insect Behavior, 29, 325-339. doi: 10.1007/s10905-016-9561-5