Polymorphism of the Second Exon of MHC-DRB Gene in Jabal-Barez Red Goat

Document Type : Research Paper

Authors

1 Academic member of Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman

2 MSc student of Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman

Abstract

More than 450000 Jabalbarez Red Goat are breeding in the cities of Jiroft and Kahnooj and produce cashmere,
meat and dairy products. In this study which was carried out to analyse the polymorphism of GoLA-DRB3
gene in Jabalbarez Red goat using PCR-RELP, blood samples were randomly and individually taken from 100
goats. Then DNA was extracted from blood samples using DIAtom DNA prep Kit and its quantities and
qualities were determined. Exon 2 of DRB 3.2 gene encompassing 285 bp amplified with heminested-PCR
method in two rounds and PCR products were digested into fragments at 122bp and 163bp (T restriction
pattern) or an undigested fragment at 285bp (t restriction patten). The Population was hardy-Weinberg
equilibrium (P<0.05). Shanon Index, Nei index, observed heterozygosity and expected heterozygosity were
calculated 0.69, 0.50, 0.52 and 0.50 respectively. Results show that the studied population has good genetic
diversity, and the efficiency of GOLA-DRB3 marker for determination of genetic diversity is good. By using
the results of this study and previous studies and the application of quantitative records and information for
locus of this population, loci affecting quantitative traits can be detected.

Keywords

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Animal Production Research
Volume 1, Issue 2 - Serial Number 2
September 2012
Pages 1-8

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