Effect of different sources of omega-3 and omega-6 calcium fat powder on performance, hatchability, serum lipids and ovarian follicles count in old broiler breeder hen

Document Type : Research Paper

Authors

1 Ph.D Student of Animal Nutrition, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Professor, Department of Animal Science, College of Agriculture and Natural Sciences, University of Tehran, Karaj, Iran

4 Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

This study was conducted to determine the effect of different levels of omega-3 and omega-6 calcium fat powders on performance, hatchability, serum lipids, abdominal fat, and ovarian follicle count in Ross-308 broiler breeder hens (65 week-old). The experiment was performed in a repeated measurement design with 84 hens and 28 roosters with seven treatments (four replicates) during three months. The experimental groups included control group (no fat powder), 1.5 and 3% of animal omega-3 calcium fat powder of fish oil origin (ω3F), plant omega-3 calcium fat powder of linseed oil origin (ω3L), and omega-6 calcium fat powder of soybean oil origin (ω6S). The results showed that the highest average of HDEP was belonged to the experimental groups containing 1.5% ω3F (61.98), and ω3L (60.25) without significant difference with together (P> 0.05). However, among experimental groups, the highest egg mass (44.78 g/h/d) was obtained from consumption of 1.5% ω3F (P<0.05). The highest concentrations of triglycerides (1806.8 mg/dL), cholesterol (206.25 mg/dL), and low density lipoprotein (244/25 mg/dL) of serum were belonged to control group that was significantly different from other experimental groups (P<0.05). The percentage of hatchability of fertile eggs was improved by consuming diets containing fat powder, except 3% ω6S, when compared to control (P<0.05). Overall, considering the production performance, hatchability and economic explanation of diets, it seems that addition of omega-3 calcium fat powder at the level of 1.5% (ω3F and ω3L) is affordable and can have a positive effect on old broiler breeder hens.

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Abbasi F., Samadi F., Jafari S. M., Ramezanpour S. and Shams Shargh M. 2019. Production of omega-3-enriched meat through feeding broilers with ultrasonicated flaxseed oil nanoemulsions: Performance, serum composition, physicochemical properties and oxidative stability. Iranian Journal of Applied Animal Science, 9(3): 487-496.
Al-Daraji H. J., Al-Mashadani H. A., Al-Hayani W. K., Mirza H. A. and Al-Hassani A. S. 2010. Effect of dietary supplementation with different oils on productive and reproductive performance of quail. International Journal of Poultry Science, 9(5): 429-435.
Al-Hilali A. H. 2018. Effect of dietary flaxseed oil on growth performance and serum lipid profiles in broilers. Pakistan Journal of Nutrition, 17(11): 512-517.
Alagawany M., Elnesr S. S., Farag M. R., Abd El-Hack M. E., Khafaga A. F., Taha A. E., Tiwari R., Yatoo M., Bhatt P., Khurana S. K. and Dhama K. 2019. Omega-3 and Omega-6 fatty acids in poultry nutrition: effect on production performance and health. Animals, 9(8): 573.
Bozkurt M., Cabuk M. and Alcicek A. 2008. Effect of dietary fat type on broiler breeder performance and hatching egg characteristics. Journal of Applied Poultry Research, 17(1): 47-53.
Ceylan N., Ciftçi I., Mizrak C., Kahraman Z. and Efil H. 2011. Influence of different dietary oil sources on performance and fatty acid profile of egg yolk in laying hens. Journal of Animal and Feed Sciences, 20(1): 71-83.
Chashnidel Y., Moravej H., Towhidi A., Asadi F. and Zeinodini S. 2010. Influence of different levels of n-3 supplemented (fish oil) diet on performance, carcass quality and fat status in broilers. African Journal of Biotechnology, 9(5): 687-691.
Delezie E., Koppenol A., Buyse J. and Everaert N. 2016. Can breeder reproductive status, performance and egg quality be enhanced by supplementation and transition of n‐3 fatty acids?. Journal of Animal Physiology and Animal Nutrition, 100(4): 707-714.
Ebeid T. A. 2011. The impact of incorporation of n-3 fatty acids into eggs on ovarian follicular development, immune response, antioxidative status and tibial bone characteristics in aged laying hens. Animal, 5(10): 1554-1562.
El-Hamid I. A., El-Din A. N., Zaghloul A. A., El-Bahrawy K. A., Elshahawy I. I., Allam A. M., El-Zarkouny S. Z. and Hassan G. A. 2016. Effects of calcium salts of fatty acids rich in palmitic and oleic fatty acids on reproduction and serum biochemistry in Barki ewes. Small Ruminant Research, 144: 113-118.
El-Katcha M. I., El-Kholy M. E., Soltan M. A. and EL-Gayar A. H. 2014. Effect of dietary omega-3 to omega-6 ratio on growth performance, immune response, carcass traits and meat fatty acids profile of broiler chickens. Poultry Science Journal, 2(2): 71-94.
Feng Y., Ding Y., Liu J., Tian Y., Yang Y., Guan S. and Zhang C. 2015. Effects of dietary omega-3/omega-6 fatty acid ratios on reproduction in the young breeder rooster. BMC Veterinary Research, 11(1): 73.
Fernandes J. I. M., Bordignon H. L. F., Prokoski K., Kosmann R. C., Vanroo E. and Murakami A. E. 2018. Supplementation of broiler breeders with fat sources and vitamin e: carry over effect on performance, carcass yield, and meat quality offspring. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70 (3): 983-992.
Fouad A. M. and El-Senousey H. K. 2014. Nutritional factors affecting abdominal fat deposition in poultry: a review. Asian-Australasian Journal of Animal Sciences, 27(7): 1057-1068.
Gilbert A. B., Perry M. M., Waddington D. and Hardie M. A. 1983. Role of atresia in establishing the follicular hierarchy in the ovary of the domestic hen (Gallus domesticus). Reproduction, 69(1): 221-227.
Goldberg E. M., Ryland D., Gibson R. A., Aliani M. and House J. D. 2013. Designer laying hen diets to improve egg fatty acid profile and maintain sensory quality. Food Science and Nutrition, 1(4): 324-335.
González-Muñoz M. J., Bastida S., Jiménez O., Vergara G. and Sánchez-Muniz F. J. 2009. The effect of dietary fat on the fatty acid composition and cholesterol content of Hy-line and Warren hen eggs. Grasas y Aceites, 60(4): 350-359.
Güçlü B. K., Uyanık F. and İşcan, K. M. 2008. Effects of dietary oil sources on egg quality, fatty acid composition of eggs and blood lipids in laying quail. South African Journal of Animal Science, 38(2): 91-100.
Gulliver C. E., Friend M. A., King B. J. and Clayton E. H. 2012. The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle. Animal Reproduction Science, 131(1-2): 9-22.
Hassan M. S. H., Radwan N. L., Khalek A. M. A. and El-Samad M. H. A. 2011. Effect of different dietary linoleic acid to linolenic acid ratios on some productive, immunological and physiological traits of Dandarawy chicks. Egyptian Poultry Science Journal, 31(1): 149-160.
Ibrahim D., El-Sayed R., Khater S. I., Said E. N. and El-Mandrawy S. A. 2018. Changing dietary n-6: n-3 ratio using different oil sources affects performance, behavior, cytokines mRNA expression and meat fatty acid profile of broiler chickens. Animal Nutrition, 4(1): 44-51.
Khatibjoo A., Kermanshahi H., Golian A. and Zaghari M. 2018. The effect of n‐6/n‐3 fatty acid ratios on broiler breeder performance, hatchability, fatty acid profile and reproduction. Journal of Animal Physiology and Animal Nutrition, 102(4): 986-998.
Kim Y. J., Park K. E., Kim Y. Y., Kim H., Ku S. Y., Suh C. S., Kim S. H. and Choi Y. M. 2017. Effects of estradiol on the paracrine regulator expression of in vitro maturated murine ovarian follicles. Tissue Engineering and Regenerative Medicine, 14(1): 31-38.
King’Ori A. M. 2011. Review of the factors that influence egg fertility and hatchability in poultry. International Journal of Poultry Science, 10(6): 483-492.
Koppenol A., Delezie E., Aerts J., Willems E., Wang Y., Franssens L., Everaert N. and Buyse J. 2014. Effect of the ratio of dietary n-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid on broiler breeder performance, egg quality, and yolk fatty acid composition at different breeder ages. Poultry Science, 93(3): 564-573.
Koppenol A., Buyse J., Everaert N., Willems E., Wang Y., Franssens L. and Delezie E. 2015. Transition of maternal dietary n-3 fatty acids from the yolk to the liver of broiler breeder progeny via the residual yolk sac. Poultry Science, 94(1): 43-52.
Mala S., Slezáčková I., Strakova E., Suchý P. and Večerek V. 2004. Plant-based diets containing Ca-salts of fatty acids and their influence on performance, carcass characteristics, and health status of broiler chickens. Acta Veterinaria Brno, 73(3): 321-328. 
Management Guide of the Ross 308 parents Stock. 2018. (http://en.aviagen.com/ross-308).
Mellouk N., Ramé C., Marchand M., Staub C., Touzé J. L., Venturi É., Mercerand F., Travel A., Chartrin P., Lecompte F. and Ma L. 2018. Effect of different levels of feed restriction and fish oil fatty acid supplementation on fat deposition by using different techniques, plasma levels and mRNA expression of several adipokines in broiler breeder hens. PloS ONE, 13(1): p.e0191121.
Monfaredi A., Rezaei M. and Sayyahzadeh H. 2011. Effect of supplemental fat in low energy diets on some blood parameters and carcass characteristics of broiler chicks. South African Journal of Animal Science, 41(1): 24-32.
Manohar G. R. 2017. Effect of dietary omega-3 fatty acid rich oil sources on fertility and hatchability performance of Japanese quail eggs. International Journal of Science, Environment and Technology, 6(1): 923-926.
Murali P., George S. K., Ally K. and Dipu M.T. 2015. Effect of L-carnitine supplementation on growth performance, nutrient utilization, and nitrogen balance of broilers fed with animal fat. Veterinary World, 8(4): 482.
Murata L. S., Ariki J., Machado C. R., da Silva L. D. P. and Rezende M. J. M. 2003. Effect of oils sources on blood lipid parameters of commercial laying hens. Brazilian Journal of Poultry Science, 5(3): 203-206.
Nateghi R., Alizadeh A., Jafari Ahangari Y., Fathi R. and Akhlaghi A., 2019. Stimulatory effects of fish oil and vitamin E on ovarian function of laying hen. Italian Journal of Animal Science, 18(1): 636-645.
Olubowale O. S., De Witt F. H., Greyling J. P. C., Hugo A., Jooste A. M. and Raito M. B. 2014. The effect of dietary lipid sources on layer fertility and hatchability. South African Journal of Animal Science, 44(5): 44-50.
Ponter A. A., Parsy A. E., Saadé M., Mialot J. P., Ficheux C., Duvaux-Ponter C. and Grimard B. 2006. Effect of a supplement rich in linolenic acid added to the diet of postpartum dairy cows on ovarian follicle growth, and milk and plasma fatty acid compositions. Reproduction Nutrition Development, 46(1): 19-29.
Radwan N. L., El-Samad M. A. and Nada S. A. 2012. Effects of different dietary ratios of linoleic acid to α-linolenic acid on productive performance, immunity of laying hens and egg yolk fatty acid composition. Egyptian Poultry Science Journal, 32(1): 163-188.
Rebolé A., Rodriguez M. L., Ortiz L. T., Alzueta C., Centeno C., Viveros A., Brenes A. and Arija I. 2006. Effect of dietary high-oleic acid sunflower seed, palm oil and vitamin E supplementation on broiler performance, fatty acid composition and oxidation susceptibility of meat. British Poultry Science, 47(5): 581-591.
Rosenfeld C. S., Wagner J. S., Roberts R. M. and Lubahn D. B. 2001. Intraovarian actions of oestrogen. Reproduction, 122(2): 215-226.
Saber S. N. and Kutlu H. R. 2018. Effect of omega-3 and omega-6 fatty acid inclusion in broiler breeder's diet on laying performance, egg quality, and yolk fatty acids composition. Indian Journal of Animal Science, 88(12): 1374-1378.
Saber S. N. and Kutlu H. R. 2019. Effect of including n-3/n-6 fatty acid feed sources in diet on fertility and hatchability of broiler breeders and post-hatch performance and carcass parameters of progeny. Asian-Australasian Journal of Animal Sciences, 33(2): 305-312.
Saleh A. A. 2013. Effects of fish oil on the production performances, polyunsaturated fatty acids and cholesterol levels of yolk in hens. Emirates Journal of Food and Agriculture, 25(89): 605-612.
Saleh H., Rahimi S. and Karimi T. M. 2009. The effect of diet that contained fish oil on performance, serum parameters, the immune system and the fatty acid composition of meat in broilers. International Journal of Veterinary Science, 3 (2): 69-75.
SAS. 2013. Base SAS 9.4 procedures guide: statistical procedures. Cary, NC, USA: SAS Institute Inc.
Viveros A., Ortiz L. T., Rodríguez M. L., Rebolé A., Alzueta C., Arija I., Centeno C. and Brenes A. 2009. Interaction of dietary high-oleic-acid sunflower hulls and different fat sources in broiler chickens. Poultry Science, 88(1): 141-151.
Wathes D. C., Abayasekara D. R. E. and Aitken R. J. 2007. Polyunsaturated fatty acids in male and female reproduction. Biology of Reproduction, 77(2): 190-201.
Wongsuthavas S., Yuangklang C., Vasupen K., Mitchaotha J., Alhaidary A., Mohamed H. E. and Beynen A. C. 2011. Fatty acid metabolism in broiler chickens fed diets either rich in linoleic or alpha-linolenic acid. Asian Journal of Animal and Veterinary Advances, 6(3): 282-289.
Yang X., Sheng W., Sun G. Y. and Lee J. C. M. 2011. Effects of fatty acid unsaturation numbers on membrane fluidity and α-secretase-dependent amyloid precursor protein processing. Neurochemistry International, 58(3): 321-329.
Zanussi H. P., Shariatmadari F., Sharafi M. and Ahmadi H. 2019. Dietary supplementation with flaxseed oil as source of omega-3 fatty acids improves seminal quality and reproductive performance in aged broiler breeder roosters. Theriogenology, 130: 41-48.
Zeron Y., Sklan D. and Arav A. 2002. Effect of polyunsaturated fatty acid supplementation on biophysical parameters and chilling sensitivity of ewe oocytes. Molecular Reproduction and Development, 61(2): 271-278.