Effect of vitamins thiamine and riboflavin on population growth, functional traits, and body fat and protein reserves in Iranian honey bee (Apis mellifera meda) colonies

Document Type : Research Paper

Authors

1 Former MSc Student, Animal Science Department, Urmia University, Urmia, Iran

2 Associate Professor, Animal Science Department, Urmia University, Urmia, Iran

3 Assistant Professor, Animal Science Research Department, Kurdistan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Sanandaj, Iran

Abstract

Introduction: Honey bees need nutrients such as protein, carbohydrates, lipids, minerals, and vitamins. Among nutrients, vitamins are particularly important for their roles in brood rearing, hypopharyngeal gland development, ovary development, longevity, bee immunity, bee weight, and flight muscle development. The vitamins stored in the body of the bees are vitally important in strengthening the immune system, normal growth, and development of broods, producing royal jelly, longevity of bees, and especially wintering quality of honey bees. Among the vitamins required by the honey bees, the most important are thiamine and riboflavin. Pollen quality depends on the supply of vitamins required by honey bees. Most pollen grains are poor and deficient in thiamine and riboflavin. This study aimed to investigate the effect of thiamine and riboflavin on population growth, functional traits, and fat and protein reserves of the bee body in Iranian honey bee colonies.
Materials and methods: An experiment was conducted in a completely randomized design with four treatments and seven repetitions from April 2022 to September 2023 in the climatic conditions of Kurdistan province, Iran. Experimental treatments included the sugar syrup (control), thiamine (1500 ppm), riboflavin (1500 ppm), and the combination of thiamine and riboflavin (1500 ppm). From the beginning of 14 April 2023, experimental treatments were fed with the mentioned concentrations of vitamins thiamine, riboflavin, and their combination of 0.5 liters every other day for 45 days and 15 days without feeding. Then, traits such as honey production, pollen collection, population (adults and broods), and protein and fat content of carcass were measured in experimental treatments.
Results and discussion: The results of the effect of thiamine, riboflavin, and their combination on the population of adult bees showed that the addition of thiamine and riboflavin in the bees' nutrition was significantly effective in the increase of adult bees' population in the investigated periods as well as the average of the entire period (P<0.05). A comparison of the averages of the experimental treatments revealed that the highest and lowest adult bee populations were associated with the treatments fed the vitamin combination and the control group, respectively. The results of the effect of thiamine, riboflavin, and their combination on the population of broods showed that the addition of thiamine and riboflavin in the diet of bee colonies during the studied periods did not have a significant effect on the increase in the population of newborns, but it showed a significant effect on the average of the entire period (P<0.05). The results of the mean comparison showed that the highest population of broods in the studied honey bee colonies was related to the treatments fed with the combination of thiamine and riboflavin, and the lowest population of broods was related to the control treatment. The results of the effect of thiamine and riboflavin on honey and pollen production traits showed that the use of vitamins and their combination in honey bee nutrition caused a significant increase in honey and pollen production traits of experimental treatments (P<0.05). The results of the mean comparison showed that the highest and lowest amounts of honey and pollen production of the studied honey bee colonies were observed in the treatment fed with the combination of vitamins and the control group, respectively. The effect of thiamine and riboflavin on the carcass protein and fat showed that the use of thiamine, riboflavin, and their combination in feeding the colonies had a significant effect on the amount of carcass protein and fat (P<0.01). A comparison of treatments showed that honey bees in the treatment fed the vitamin combination had the highest carcass protein and fat, and honey bees in the control treatment had the lowest carcass fat and protein.
Conclusions: Based on the results of the present experiment, it could be concluded that the use of thiamine and riboflavin, and their combination in feeding honey bees had a positive effect on population growth, functional traits, and fat and protein reserves of the bees' body in the colonies and improved the performance and increased the economic efficiency of the colony.

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References

Anderson, J., & A. Dietz. (1976). pyridoxine requirement of the honey bee (Apis mellifera) for brood rearing. Apidologie, 7, 67-84.
Back, E. (1956). Influence of the vitamins in pollen on the longevity of honey bees, the development of their pharyngeal glands and their ability to rear brood. Insectes Socianx, 3, 285-292.
Bamidele, J. A., Idowu, A. B., Ademolu, K., & Osipitan, A. A. (2021). Nutritional composition of Apis mellifera. (Hymenoptera: Apidae) from three ecological zones of Nigeria. Journal of Apiculture Research, 60(3), 445-56. doi: 10.1080/00218839.2021.1874709
Brodschneider, R., & Crailsheim, K. (2010). Nutrition and health in honey bees. Apidologie, 41(3), 278-294. doi: 10.1051/apido/2010012
Chaichi Mansour, S., Alou Qhutbi, A., & Nehzati Paghaleh, G. (2014). Different levels of multivitamins affect the number of eggs, larvae, and pupae (rearing babies), population growth, percentage of fat and protein in worker carcasses, and wintering of honey bee colonies.  In: Proceeding of 8th National Congress on Livestock and Poultry Nutrition, 4 -5 March., Iran. Pp. 507-514. [In Persian]
Crailsheim, K. (2009). Nutrition and Health in Honey Bees. Apidologie, 41, 278–294. doi:10.1051/apido/2010012
Darat, M., Tahmasbi, G. H., & Zareei, L. (2017). The effects of different levels of vitamin E on Performance and reproductive traits of Iranian honeybee colonies. Iranian Honey Bee Science and Technology, 8(14), 1-12. doi: 10.22092/HBSJ.2017.111892
De Grandi-Hoffman, G., Wardell, G., Ahumada-Secura, F., Rinderer, T. E., Danka, R., & Pettis, J. (2008). Comparisons of pollen substitute diets for honeybees: consumption rates by colonies and effects on brood and adult populations. Journal of Apiculture Research, 47, 265-270. doi: 10.3896/IBRA.1.47.4.06
Elbassiouny, A. M. (2006). Effect of vitamin additive and colony management on honey bee performance. Arab Universities Journal of Agricultural Sciences, 14(1), 427-438. doi: 10.21608/AJS.2006.15578
Frizzera, D., Del Fabbro, S., Ors, G., Zanni, V., Bortolomeazzi, R., Nazzi, F., & Annoscia, D. (2020). Possible side effects of sugar supplementary nutrition on honey bee health. Apidologie, 51(4), 594-608. doi: 10.1007/s13592-020-00745-6
Forghani, M. A., Tahmasbi, G. H., & Foladee, H. (2007). A study on the nutritional effect of thiamine (B1) and pantothenic acid (B5) supplements on royal jelly production and population growth in honeybee colonies (Apis mellifera). Proceedings of the 6th Iranian Honeybee Seminar, November 1, 2007, Agricultural Education Publications, Karaj, Iran. P. 145. [In Persian]
Ghosh, S., Jung, C., & Meyer-Rochow, V. B. (2016). Nutritional value and chemical
composition of larvae, pupae, and adults of worker honey bee, Apis mellifera ligustica a sustainable food source.  Journal of Asia-Pacific Entomology, 19, 487-495. doi: 10.1016/j.aspen.2016.03.008
Glavinic, U., Stankovic, B., Draskovic, V., Stevanovic, J., Petrovic, T., Lakic, N., & Stanimirovic, Z. (2017). Dietary amino acid and vitamin complex protects honey bees from immunosuppression caused by Nosema ceranae. PloS One, 12(11), e0187726. doi: 10.1371/journal.pone.0187726
Ghornani, V. (2016). Investigating the effect of mineral nanosupplements on honey production, population growth, and increasing brood rearing in honey bee colonies. Ph.D. dissertation. University of Tehran, Iran.
Hagedorn, H. H., Maferof, M., & Moeller, F. E. (2015). Effect of the age of pollen used in pollen supplements on their nutritional value for the honeybee. I. Effect on thoracic weight, development of hypopharyngeal glands, and brood rearing. Journal of Apicultural Research, 7(2), 89-95. doi: 10.1080/00218839.1968.11100195
Herbert Jr, E. W., & Shimanuki, H. (1978). Mineral requirements for brood-rearing by honeybees fed a synthetic diet. Journal of Apicultural Research, 17(3), 118-122. doi: 10.1080/00218839.1978.11099916
Hrassnigg, N., Leonhard, B., & Crailsheim, K. (2003). Free amino acids in the haemolymph of honeybee queens. Amino Acids, 24, 205-212.  doi: 10.1007/s00726-002-0311-y
Huang, Z. (2010). Honey bee nutrition. American Bee Journal, 138, 22-26.
Jafariani, A. (2021). The effect of B vitamins on queen egg laying, colony population growth, honey bee queen egg laying. MSc thesis. University of Guilan, Iran. [In Persian]
Krol, A. (1993). Effect of dietary vitamin B1 on the condition and development of honeybees. Pszczelnicze-zeszyty-naukowe Poland, 37, 11-21.
Mattila, H. R., & Otis, G. W. (2006). The effects of pollen availability during larval development on the behavior and physiology of spring-reared honey bee. Apidologie, 37, 533-546. doi: 10.1051/apido:2006037
Mollud Poor, V. (2019). Investigating the effect of mineral nano supplements on honey production, population growth, and increasing brood rearing in bee colonies. Ph.D. Thesis, University of Tehran, Iran. [In Persian]
Morais, M., Turcatto, A. P., Francoy, T. M., Gonçalves, L. S., Cappelari, F. A., & Jong, D. (2013). Evaluation of inexpensive pollen substitute diets through quantification of hemolymph proteins. Journal of Apicultural Research, 52(3), 119-121. doi: 10.3896/IBRA.1.52.3.01
Nahzati, G. (2009). Digestion study of several protein supplements in honey bees. Ph.D. Thesis, University of Tehran. [In Persian]
Nawaz, M., Ashfaq, M., & Ali, A. (2008). Studies on improvement of artificial diet and its effect on biological characters of Chrysoperla carnea Stephens. Pakistan Journal of Entomology, 30(1), 73-76.
Page, R., Laidlaw, H., & Erickson, E. (2002). Closed population honeybee breeding 4. The distribution of sex alleles with top crossing. Journal of Apicultural Research, 24(1), 38-42. doi: 10.1080/00218839.1985.11100646
Pryor, W. A. (2000). Vitamin E and heart disease: Basic science to clinical intervention trials. Free Radical Biology and Medicine, 28, 141-164. doi: 10.1016/s0891-5849(99)00224-5
Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L., & Jamali, J. (2017). Morphological diversity and phylogenetic relationships Study of Iranian subspecies honey bee (Apis mellifera meda) populations via morphological characteristics. Sociobiology, 64(1), 33-41. doi: 10.13102/sociobiology.v64i1.1179
Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L., & Jamali, J. (2018). Genetic variation in Iranian honeybees, Apis mellifera meda Skorikow, 1829, (Hymenoptera: Apidae) inferred from RFLP analysis of two mtDNA regions (COI and 16S rDNA). Sociobiology, 65(3), 482-490. doi: 10.13102/sociobiology.v65i3.2876
Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L., & Jamali, J. (2019). Phylogenetic relationship study of Iranian subspecies honeybee with other honeybee subspecies using morphological and molecular markers. Animal Science Journal (Pajouhesh & Sazandegi), 123, 323-334. doi: 10.22092/asj.2018.121251.1676 [In Persian]
Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L., & Jamali, J. (2021). Genetic diversity evaluation of Iranian honeybee (Apis mellifera meda) populations based on PCR-RFLP marker analyses. Modern Genetics Journal,16(3), 219-233. [In Persian]
Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L., & Jamali, J. (2022). Molecular genetic diversity and population structure of Iranian honey bee (Apis mellifera meda) populations: Implications for breeding and conservation. Journal of Plant Diseases and Protection, 129(4), 1-12. doi: 10.1007/s41348-022-00657-w
Rahimi, A., Tahmasebi, G. H., Bahmani, H. R., Salehi, S., Zare, B., Parsanaseb, A., & Rokhzad, B. (2023a). Comparative Evaluation of Performance for Improved Iranian Honey Bee queens (Apis mellifera meda Skorikov 1929) in the Climate Conditions of Kurdistan Province. Research on Animal production. 14(39), 102-111. doi: 20.1001.1.122518622.1402.14.39.11.2 [In Persian]
Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L., & Jamali, J. (2023b). Genetic characterization of Iranian honeybees, Apis mellifera meda Skorikow, 1829, based on microsatellite DNA polymorphism. Biochemical Genetics, 61(2), 1-25. doi: 10.1007/s10528-023-10368-y
Rezvan, M., Dashab, G. R., Elmi, M., Tajabadi, N., Shojayan, K., & Behjatuan Esfahani, M. (2023). Investigating the effect of sweet paste containing AVP-4 nanosupplement on queen egg laying rate, population size, and carcass biochemical characteristics in honey bee (Apis mellifera) colonies. Animal Science Journal, 139, 123-134. doi: 10.22092/ASJ.2022.360217.2261 [In Persian]
Robinson, A. (2005). Worker nutrition and division of labor in honeybees. Animal Behavior, 69, 427-435.
Sharma, V. P., & Kumar, N. R. (2010). Changes in honeybee behavior and biology under the influence of cellphone radiation. Current Science (Bangalore), 98(10), 1376-1378.
Somerville, D. (2005). Honey bee nutrition and supplementary feeding. Agnote Nsw Agriculture, 178, 1-8.
Somerville, D., & Nicol, H. I. (2022). Mineral content of honey bee-collected pollen from southern New South Wales. Australian Journal of Experimental Agriculture, 42(8), 1131-1136. doi: 10.1071/EA01086
Tahmasebi, G., Ebadi, R., Baneh, H., Parichehreh, Sh., Babaei, M., Seyfie, E., & Sartippour, A (2022). The variation trend of the functional traits of improved queens of Iranian honeybees (Apis mellifera meda) during four generations in different private apiaries. Animal Production Research, 11, 91-105. doi: 10.22124/AR.2022.15688.1534 [In Persian]
Yarahamdi, S., Miraei Ashtiani, S., Ebadi, R., & Tahmasabi, G. (2007). Phenotypic correlation between nine morphological traits and three production traits in the population of honey bees in Tehran province. Agricultural Sciences and Techniques and Natural Resources, 5(2), 157-168. [In Persian]
Wilde, J., Siuda, M., & Bak, B. (2014). Development and productivity of honeybee colonies administered food supplements in spring. Medycyna Weterynaryjna, 70(12), 750-753.
Animal Production Research
Volume 13, Issue 2
August 2024
Pages 87-98

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