شناسایی چندشکلی‌های تک‌نوکلئوتیدی ژن PPP2CA و اثر آن بر صفات لاشه، رشد و ذخیره چربی در گوسفندان لری‌بختیاری و زل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی دانشگاه تهران

2 دانشیار گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران

3 استاد گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران

چکیده

هدف این مطالعه، تعیین چندشکلی‌های ژن PPP2CA در کروموزوم 5 گوسفند و اثر آن بر صفات لاشه، رشد و ذخیره چربی در گوسفندان لری بختیاری و زل بود. از 140 رأس گوسفند زل (اصلاحی یا کشتاری) و 165 رأس گوسفند لری‌بختیاری خون‌گیری و استخراج DNA به عمل آمد. سپس قطعه 243 جفت بازی از اگزون یک ژن PPP2CA تکثیر و الگوهای مختلف آن توالی‌یابی شد. دو الگوی باندی متفاوت مشاهده شد و نتایج توالی‌یابی وجود دو جهش را نشان داد. در گروه گوسفندان زل اصلاحی و لری‌بختیاری، اثر ژنوتیپ‌های T/T و Del/Del بر صفت وزن از شیرگیری (kg 46/1±67/30 در برابر kg 52/1± 67/30) و کلسترول (%94/0±76/60 در برابر % 65/1± 26/54) معنی‌دار بود (05/0P<). در نژاد لری بختیاری، ژنوتیپ Del/Del به وزن دنبه بیشتری (kg21/0±20/5) نسبت به ژنوتیپ T/T (kg12/0±28/3) منجر شد (05/0P<). در زل کشتاری بین اثر ژنوتیپ‌ها بر درصد چربی لاشه و تری­گلیسرید خون تفاوت معنی­داری وجود داشت، به طوری که ژنوتیپ T/T درصد چربی لاشه بیشتر (05/0P<) و ژنوتیپ Del/Del تری­گلیسرید خون بالاتری داشت (01/0P<). بر اساس نتایج این تحقیق، می­توان انتظار داشت که این ژن در صفات چربی تاثیرگذار بوده و باید در برنامه­ های اصلاح نژادی مورد توجه قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification of single nucleotide polymorphisms of PPP2CA gene and its effect on carcass, growth and fat storage traits in Lori Bakhtiari and Zel sheep

نویسندگان [English]

  • M. Zare 1
  • M. Sadeghi 2
  • M. Moradi-Shahrbabak 3
1 M.Sc. gratuated of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran .
2 Associate professor of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
3 Professor of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
چکیده [English]

The purpose of this study was to determine the polymorphism of PPP2CA gene in chromosome 5 of sheep and its effect on carcass, growth and fat storage traits in Lori-Bakhtiari and Zel sheep. Blood samples were collected from 140 Zel sheep (breeding or slaughtering), and 165 Lori-Bakhtiari sheep, and their DNA was extracted. Then, the fragment of 243 bp of exon 1 of PPP2CA gene was amplified of and its various patterns were sequenced. Two different banding patterns were observed and the results of sequencing showed existence of two mutations. Within the group of breeding Zel and Lori-Bakhtiari, the effect of T/T and Del/Del genotypes on weaning weight (27.79±1.46 vs 30.67 ± 1.57) and cholesterol (60.76±0.94 vs 54.26±1.65) was significant (P<0.05). In Lori-Bakhtiari, Del/Del genotype resulted in heavier fat tail (5.20±0.21kg) than T/T genotype (5.20±0.21kg vs 3.28±0.12kg) (P<0.05). In slaughtering Zel, the effect of genotypes on carcass fat percentage and triglyceride was significant, so that the T/T genotype had more carcass fat percentage (P<0.05) and Del/Del genotype had higher triglyceride (P<0.01). On the basis of the results, it is expected that this gene is affective on fat traits and should be considered in breeding programs.

کلیدواژه‌ها [English]

  • Gene PPP2CA
  • Lori-Bakhtiari sheep
  • Zel sheep and Indels

مراجع

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تحقیقات تولیدات دامی
دوره 5، شماره 4
اسفند 1395
صفحه 70-78

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