اثر تزریق درون آمنیوتیکی روی - متیونین و نانو روی - متیونین بر بیان ژن Zn-T1 و فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز روده کوچک جوجه‌های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

2 دانشیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

3 استادیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

چکیده

هدف از این مطالعه بررسی اثر تزریق درون­آمنیوتیکی روی - متیونین و نانو روی - متیونین بر بیان ژن انتقال­دهنده روی (Zn-T1) و فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز در ژژنوم جوجه‌های گوشتی بود. چهارصد عدد تخم‌ مرغ بارور به چهار گروه (تیمار)، هریک با چهار زیر گروه (تکرار) تقسیم شد. تیمار اول تا سوم به ترتیبت شامل تزریق درون­آمنیوتیکی یک میلی­لیتر محلول سرم فیزیولوژی، سرم فیزیولوژی حاوی ۲۵% روی - متیونین و ۲۵% نانو روی - متیونین در روز هفدهم جوجه­کشی بود. چهارمین تیمار (کنترل منفی) هیچ تزریقی دریافت نکرد. درصد تفریخ هر تیمار محاسبه و در روزهای اول، سوم و هفتم پرورش، دو پرنده از هر تکرار ذبح شد و بیان ژن Zn-T1، فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز در بافت روده اندازه‌گیری شد. وزن جوجه‌ها در زمان تفریخ در تیمار روی - متیونین (۰۶/۰±۰۲/۴۳) و نانو روی - متیونین (۰۶/۰±۷۷/۴۲) از سایر تیمارها بیشتر بود (۰۱/۰P˂). همچنین وزن روده کوچک در روزهای یک و هفت پس از تفریخ در تیمار روی - متیونین (به ترتیب ۰۴۸/۰±۷/۹ ، ۰۷/۰±۵۵/۱) و نانو روی - متیونین (به ترتیب ۰۴۸/۰±۵/۹ ، ۰۷/۰±۵۱/۱) از سایر تیمارها بیشتر بود (۰۱/۰P˂). تیمار نانو روی - متیونین در روزهای یک، سه و هفت بعد از تفریخ، بیشترین فعالیت آنزیم آلکالین فسفاتاز (به ترتیب ۱/۰±۸/۱۷، ۰۸/۰±۵/۱۱، ۰۴/۰±۲/۷) مالتاز (به ترتیب ۰۶/۰±۸/۸ ،۰۲/۰±۵/۵ ،۰۳/۰±۲/۴) و بیان ژن Zn-T1 (به ترتیب ۰۰۹/۰±۱۷/۳ ،۰۰۷/۰±۲۴/۴ ،۰۰۵/۰±۶۷/۳) را نشان داد (۰۱/۰P˂). استنتاج نهایی این است که تزریق درون تخم‌مرغی نانو روی - متیونین منجر به افزایش بیان Zn-T1 شده و فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز روده را در هفته اول پرورش افزایش می‌دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of In ovo injection of zinc-methionine and nano-zinc methionine on the Zn-T1 gene expression, alkaline phosphatase and maltase activity in broilers small intestine

نویسندگان [English]

  • K. Razani 1
  • M. Mottaghitalab 2
  • S. H. Hossieni Moghaddam 3
1 Ph.D student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Assistant professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

The aim of this study was to investigate the effect of intra amniotic injection of zinc-methionine and nano-zinc-methionine on intestinal zinc transporter (zn-t1)expression and alkaline phosphatase and maltase activity in the broiler jejunum. Four hundred fertile eggs were divided into four groups (treatment), each with four samples (replicate). The first three treatments were subject to intra amniotic injection of one milliliter of physiologic serum solution, physiologic serum solution containing 25% zinc- methionine and/or containing 25% nano-zinc- methionine, respectively, at the 17th day of incubation. The fourth treatment (negative control) received no injection. The hatchability percentage was calculated and two birds from each replicate were killed then intestinal alkaline phosphatase and maltase activity and Zn-T1gene expression were assayed at 1, 3 and 7 days after hatch. The highest body weight was for Zinc-methionine (43.02± 0.06) and Nano-zinc-methionine (42.77±0.06) treatments (P˂0.01). Also at 1 and 7 days of post hatch the weight of small intestine in the Zinc-methionine (1.55±0.07, 9.7±0.048) and Nano-zinc-methionine (1.51±0.07, 9.5±0.048) treatments were higher than other treatments (p˂0.01). Ten nano-zinc-methionine treatment, at 1, 3 and 7 days after hatching, had the most activity of alkaline phosphatase (7.2 ± 0.04, 11.5± 0.08, 17.8 ± 0.1) and maltase (4.2±0.03, 5.5±0.02, 8.8±0.06) andmost Zn-T1 gene expression (3.67±0.005, 4.24±0.007, 3.17±0.009) (P˂0.01). In conclusion, in Ovo injection of Nano-zinc-methionine showed up-regulate Zn-T1 gene expression and lead to increase alkaline phosphatase and maltase activity of chick intestine during first week after hatch.
 

کلیدواژه‌ها [English]

  • Alkaline phosphatase
  • Broiler
  • Maltase
  • RT-PCR
  • Zinc transporter
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