شناسایی ژن‌های متفاوت بیان شده در جوجه‌های آلوده به ویروس H5N1 با استفاده از فرا‌تحلیل داده‌های ریزآرایه DNA

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استاد دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 دانشیار پژوهشکده بیوتکنولوژی دانشگاه شیراز

4 استادیار سازمان دامپزشکی ایران

چکیده

پیدا کردن نیم‌رخ بیان ژنی در طول سال­های اخیر، منجر به شناسایی ژن­های خاص در پاسخ به بیماری­های گوناگون شده­است. یکی از این بیماری­های مهم در صنعت طیور، بیماری آنفولانزا است. هدف از پژوهش حاضر، شناسایی ژن­های موثر در بروز آنفولانزای ناشی از ویروس H5N1 با استفاده از فرا­تحلیل دو مجموعه داده­ ریزآرایه DNA که بیان ژن­های میزبان در پاسخ به آنفولانزا را مورد بررسی قرار داده بودند، بود. فراتحلیل نشان داد که ژن­های STK17B، CCL4، MCM9، IRF7 و IL6  با روش­های Fisher، AW و maxP متمایز بیان می‌شوند. برای تایید تمایز بیان ژن­های شناسایی شده در آزمایشگاه از Real-time PCR استفاده شد. بدین منظور از نمونه­هایی استفاده شد که پیش­تر با ویروس H5N1 چالش داده شده بودند. نتایج برای هر پنج ژن انتخابی، تمایز بیان آنها را در حالت مواجهه با آلودگی و حتی جهت تنظیمی بیان را که در فرا­تحلیل مشاهده شده بود، در سطوح معنی­دار تایید نمود، به گونه­ای که ژن­های IL6، IRF7 و CCL4 افزایش بیان و ژن­های STK17B و MCM9 کاهش بیان نشان دادند. این نتایج پیشنهاد می­دهند فرا­تحلیل روشی بسیار قوی در شناسایی ژن­هایی است که ممکن است در پژوهش­های انفرادی مورد توجه قرار نگیرند. این ژن­ها ممکن است نقش حیاتی در تنظیم پاسخ میزبان ایفا نمایند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification of differentially expressed genes in H5N1 infected chickens using meta-analysis of DNA microarray datasets

نویسندگان [English]

  • M. Salehinasab 1
  • Gh. Rahimi Mianji 2
  • E. Ebrahimie 3
  • S. A. Ghafouri 4
1 Ph.D student, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
2 Professor, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
3 Associate Professor, Institute of Biotechnology, Shiraz University, Shiraz, Iran
4 Assistant Professor, Iran veterinary organization, Tehran, Iran
چکیده [English]

During the last years, gene expression profiling has revealed specific genes which are involved in the regulation of host response to several diseases. Avian Influenza infection is one of the important diseases in poultry industry. The aim of the present study was to identify significantly involved genes in H5N1 infection using the meta-analysis of two DNA microarray datasets of host response to influenza infection. The meta-analysis revealed that STK17B, CCL4, MCM9, IRF7 and IL6 were differentially expressed using Fisher, AW and maxP methods. We used real-time PCR to validate the differential expression of identified genes. For this aim, the previously H5N1-infected samples were used. The real-time PCR results of all five genes confirmed the differential expression between infected and non-infected samples and also the type of regulation obtained from meta-analysis significantly. The genes IL6, IRF7 and CCL4 were over expressed while STK17B and MCM9 were down expressed in infected samples. The results suggested that the meta-analysis is a robust method to find differentially expressed genes which may be ignored in individual analysis. These genes may play some critical roles in host response.

کلیدواژه‌ها [English]

  • Avian influenza
  • Host response
  • Real-Time PCR
  • Differential expression
  • Meta-analysis

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تحقیقات تولیدات دامی
دوره 7، شماره 3
آذر 1397
صفحه 13-23

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