اثر شکل فیزیکی خوراک و نحوه عرضه آن بر عملکرد رشد، گوارش‌پذیری ظاهری مواد مغذی، رفتار تغذیه ای و فراسنجه های خونی و شکمبه ای بره های پرواری

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان

چکیده

در این پژوهش، اثر شکل فیزیکی و نحوه عرضه خوراک بر عملکرد رشد، گوارش‌پذیری ظاهری، برخی فراسنجه­های شکمبه­ای و خونی بره­های پرواری مورد بررسی قرار گرفت. 28 رأس بره نر به­مدت 75 روز با چهار جیره­ (TMR با کنسانتره مش، کنسانتره مش و علوفه به­صورت مجزا، TMR با کنسانتره پلت و کنسانتره پلت و علوفه به­صورت مجزا) تغذیه شدند. تغذیه کنسانتره پلت در دوره رشد، ضرایب گوارش‌پذیری ADF و NDF و در دوره پایانی، ضرایب گوارش‌پذیری مواد مغذی، ماده خشک و ماده آلی و ADF را افزایش داد (05/0>P). تغذیه کنسانتره مش در دوره پایانی، نیتروژن اوره­ای خون، فعالیت آلانین آمینوترانسفراز و زمان فعالیت خوردن و جویدن را افزایش داد و سبب کاهش زمان استراحت شد (05/0>P). تغذیه جداگانه علوفه و کنسانتره در دوره رشد و پایانی باعث افزایش مصرف خوراک، بهبود گوارش‌پذیری چربی خام شد و میزان pH شکمبه و فعالیت آلکالین فسفاتاز را در دوره پایانی افزایش داد. تغذیه خوراک مخلوط در دوره رشد موجب افزایش گوارش‌پذیری NDF و فعالیت آلکالین فسفاتاز و در دوره پایانی باعث بهبود گوارش‌پذیری ظاهری ADF شد (05/0>P). برهم­کنش بین نحوه فرآوری کنسانتره و عرضه خوراک بر ضرایب گوارش‌پذیری چربی خام، نیتروژن اوره­ای خون و فعالیت خوردن در دوره رشد، پروتئین خام و چربی خام دوره پایانی تاثیر داشت (05/0>P). فرآوری کنسانتره و نحوه عرضه آن و برهم­کنش بین فرآوری کنسانتره و نحوه عرضه بر روی هیچ­کدام از فراسنجه­های عملکردی تاثیر نداشت (05/0P>). نتایج نشان داد که شکل فیزیکی و نحوه عرضه خوراک بر گوارش‌پذیری ظاهری، فراسنجه‌های شکمبه‌ای و خونی و رفتار تغذیه‌ای تأثیرگذار است، اما تأثیری بر عملکرد رشد نداشت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of physical form of feed and feed delivery on performance, digestibility and nutritional behavior, and blood and rumen parameters of fattening lambs

نویسندگان [English]

  • S. Nazari Seresht
  • A. Azarfar
  • A. Fadayifar
  • Y. Beyranvand
Animal Science Department, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
چکیده [English]

Introduction: Although sheep husbandry in Iran has a long history, it is usually practiced in a traditional manner with low productivity. The growing population, increasing demand for red meat, and high costs of feed supply necessitate the use of rations with balanced nutrients, minimal waste, and maximum profitability. Therefore, nutrition management and improving productivity in this sector are of particular importance. Physical processing of feed is one of the key management strategies in this regard and is among the most important factors affecting feed intake and apparent digestibility. Feed processing and particle size modification have been shown to influence animal performance. Pelleting, grinding feed ingredients, and various processing methods applied to cereal grains and other ration components have all been employed to improve nutrient utilization efficiency in ruminant nutrition. This study investigated the effects of physical form and feeding method of diets on growth performance, apparent digestibility, and selected ruminal and blood parameters of fattening lambs.
Materials and methods: Twenty-eight Lori-Bakhtiari male lambs, with an average age of approximately three months and an average body weight of 35.8±4.4 kg, were used at the Small Ruminant Research Farm, Faculty of Agriculture, Lorestan University, Iran. The experimental period lasted 75 days, including 15 days of adaptation and 60 days of fattening. Animals were housed individually (150×100×100 cm; length×width×height) in pens equipped with water and feed buckets, and had ad libitum access to feed and water throughout the study. Four experimental diets were designed in a 2×2 factorial arrangement, consisting of two concentrate forms (pelleted and mash) and two feeding methods (total mixed ration and separate feeding). The treatments included: (1) Total mixed ration (TMR) with mash concentrate, (2) Separate feeding of mash concentrate and forage, (3) TMR with pelleted concentrate, and (4) Separate feeding of pelleted concentrate and forage. The diets for both growing and finishing periods were formulated according to NRC (2007) to meet at least the minimum nutrient requirements of the lambs. The concentrate portion of each diet was ground to 3 mm, and pelleted concentrates were processed using a 5 mm die. During the experimental period, diets were fed twice daily at 08:00 and 16:00 h. Feed intake and refusals were recorded throughout the study. Lambs were weighed on day one and subsequently every 20 days before the morning feeding after a 16-hour fasting period. Average daily gain was calculated as the difference between initial and final weights divided by the number of days. Apparent nutrient digestibility was determined using the acid-insoluble ash marker method. On day 28 of the growing phase and day 58 of the finishing phase, ruminal fluid samples were collected three hours after the morning feeding using a stomach tube. To reduce saliva contamination, the first 10–20 mL of the sample was discarded. Data were analyzed using a completely randomized design with the SAS software (version 9.4).
Results and discussion: Feeding pelleted concentrate during the growing period increased the digestibility coefficients of ADF and NDF, while in the finishing period, it improved the digestibility of nutrients, dry matter, organic matter, and ADF (P<0.05). Feeding mash concentrate during the finishing period increased blood urea nitrogen, alanine aminotransferase activity, and eating and chewing times, while reducing resting time (P<0.05). Separate feeding of forage and concentrate during both the growing and finishing periods increased feed intake, improved crude fat digestibility, and in the finishing phase, increased ruminal pH and alkaline phosphatase activity. Feeding mixed rations increased NDF digestibility and alkaline phosphatase activity during the growing phase, and improved ADF digestibility during the finishing phase (P<0.05). The interaction between concentrate processing and feeding method significantly affected crude fat digestibility, blood urea nitrogen, and eating activity in the growing period, as well as crude protein and crude fat digestibility in the finishing phase (P<0.05). However, concentrate processing, feeding method, and their interaction had no significant effect on growth performance traits (P>0.05). Overall, the results demonstrated that physical form and feeding method of the diet influenced apparent digestibility, ruminal and blood parameters, and feeding behavior, but had no effect on growth performance.
Conclusions: The results of this study indicated that although dry matter intake was higher in the separate feeding of concentrate and forage treatment, feeding a TMR with pelleted concentrate improved apparent nutrient digestibility and some metabolic parameters. These findings suggest that feed efficiency was higher in the TMR with pelleted concentrate treatment, which may be more beneficial for fattening lambs. Furthermore, feeding method significantly influenced ruminal pH during the finishing period and blood alkaline phosphatase activity during both periods, while concentrate physical form affected blood urea nitrogen and alanine aminotransferase activity in the finishing period, warranting further investigation.

کلیدواژه‌ها [English]

  • Fattening lamb
  • Physical form of feed
  • Growth performance
  • Blood parameters
  • Digestibility
Abbasi, M., Bashtani, M., Foroughi A. R., Farhangfar, H., & Ganji, F. (2016). Comparison of flour concentrate and pellet on milking performance, nutritional behaviors and some blood metabolites in dairy cows. Journal of Animal Science Research, 26(3), 151-163. [In Persian] 
Aksoy, N. H., Karaşahin, T., Dursun, Ş., Akbulut, N. K., Haydardedeoğlu, A. E., İlgün, R., & Büyükleblebici, O. (2018). Comparative investigation of some liver enzyme functions considering age and gender distinctions in healthy Akkaraman sheep. Journal of Experimental and Clinical Medicine35(3), 71-75.
Al-Hadithy, H. A. H. (2013). Estimation of serum liver enzymes activities in Awassi sheep. The Iraqi Journal of Veterinary Medicine37(1), 115-120.
Allen, M. S. (2000). Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science83(7), 1598-1624.
Atwood, S. B., Provenza, F. D., Wiedmeier, R. D., & Banner, R. E. (2001). Influence of free-choice vs mixed-ration diets on food intake and performance of fattening calves. Journal of Animal Science79(12), 3034-3040.
Beigh, Y. A., Ganai, A. M., Ahmad, H. A. (2017). Prospects of complete feed system in ruminant feeding: a review. Veterinary World, 1, 424.
Bertipaglia, L. M. A., Fondevila, M., Van Laar, H., & Castrillo, C. (2010). Effect of pelleting and pellet size of a concentrate for intensively reared beef cattle on in vitro fermentation by two different approaches. Animal Feed Science and Technology159(3-4), 88-95.
Braun, J. P., Trumel, C., & Bézille, P. (2010). Clinical biochemistry in sheep: A selected review. Small Ruminant Research92(1-3), 10-18.
Broderick, G. A., & Kang, J. H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science63(1), 64-75.
Castillo, C., Hernández, J., Méndez, J., Llena, J., Pereira, V., López-Alonso, M., & Benedito, J. L. (2006). Influence of grain processing on acid–base balance in feedlot steers. Veterinary Research Communications30, 823-837.
Chen, K. H., Huber, J. T., Theurer, C. B., Swingle, R. S., Simas, J., Chan, S. C., ... & Sullivan, J. L. (1994). Effect of steam flaking of corn and sorghum grains on performance of lactating cows. Journal of Dairy Science77(4), 1038-1043.
DeVries, T. J., & Von Keyserlingk, M. A. G. (2009). Feeding method affects the feeding behavior of growing dairy heifers. Journal of Dairy Science92(3), 1161-1168.
Dijkstra, J., Mills, J. A., & France, J. (2002). The role of dynamic modelling in understanding the microbial contribution to rumen function. Nutrition Research Reviews15(1), 67-90.
Dos Santos, W. B. R., Santos, G. T. D., da Silva-Kazama, D. C., Cecato, U., De Marchi, F. E., Visentainer, J. V., & Petit, H. V. (2011). Production performance and milk composition of grazing dairy cows fed pelleted or non-pelleted concentrates treated with or without lignosulfonate and containing ground sunflower seeds. Animal Feed Science and Technology169(3-4), 167-175.
Economides, S., Koumas, A., Georghiades, E., & Hadjipanayiotou, M. (1990). The effect of barley-sorghum grain processing and form of concentrate mixture on the performance of lambs, kids and calves. Animal Feed Science and Technology31(1-2), 105-116.
Genis, S., Verdú, M., Cucurull, J., & Devant, M. (2021). Complete feed versus concentrate and straw fed separately: Effect of feeding method on eating and sorting behavior, rumen acidosis, and digestibility in crossbred Angus bulls fed high-concentrate diets. Animal Feed Science and Technology273, 114820.
Ghassemi-Nejad, J. G., Torbatinejad, N., Naserian, A. A., Kumar, S., Kim, J. D., Song, Y. H., ... & Sung, K. I. (2012). Effects of processing of starter diets on performance, nutrient digestibility, rumen biochemical parameters and body measurements of Brown Swiss dairy calves. Asian-Australasian Journal of Animal Sciences25(7), 980.
Gimeno, A., Al Alami, A., Toral, P. G., Frutos, P., Abecia, L., Fondevila, M., & Castrillo, C. (2015). Effect of grinding or pelleting high grain maize-or barley-based concentrates on rumen environment and microbiota of beef cattle. Animal Feed Science and Technology203, 67-78.
Gordon, F. J., Patterson, D. C., Yan, T., Porter, M. G., Mayne, C. S., & Unsworth, E. F. (1995). The influence of genetic index for milk production on the response to complete diet feeding and the utilization of energy and nitrogen. Animal Science61(2), 199-210.
Hoffmann, W. E., & Solter, P. F. (2008). Diagnostic enzymology of domestic animals. Clinical Biochemistry of Domestic Animals6, 351-378.
Huntington, G. B. (1997). Starch utilization by ruminants: from basics to the bunk. Journal of Animal Science75(3), 852-867.
Iraira, S. P., De La Torre, J. R., Rodríguez-Prado, M., Manteca, X., Calsamiglia, S., & Ferret, A. (2012). Effect of feeding method on intake and behaviour of individually reared beef heifers fed a concentrate diet from 115 to 185 kg of body weight. Animal6(9), 1483-1490.
Islam, R., Redoy, M. R. A., Shuvo, A. A. S., Sarker, M. A. H., Akbar, M. A., & Al-Mamun, M. (2017). Effect of pellet from total mixed ration on growth performance, blood metabolomics, carcass and meat characteristics of Bangladeshi garole sheep. Progressive Agriculture28(3), 222-229.
Karimizadeh, E., Chaji, M., & Mohammadabadi, T. (2017). Effects of physical form of diet on nutrient digestibility, rumen fermentation, rumination, growth performance and protozoa population of finishing lambs. Animal Nutrition3(2), 139-144.
Kaufman, W. (1976). Influence of the composition of the ration and the feeding frequency on ph-regulation in the rumen and on feed in-take in ruminants. Livestock Production Science3(2), 103-114.
Keane, M. G., Drennan, M. J., & Moloney, A. P. (2006). Comparison of supplementary concentrate levels with grass silage, separate or total mixed ration feeding, and duration of finishing in beef steers. Livestock Science103(1-2), 169-180.
Ki, K. S., Kim, H. S., Jeong, H. Y., Lee, H. J., Ahn, B. S., Kim, J. S., ... & Ha, J. K. (2003). The survey of particle size of total mixed ration in Korea. Journal of Animal Science and Technology45(5), 813-818.
Kolver, E. S., & De Veth, M. J. (2002). Prediction of ruminal pH from pasture-based diets. Journal of Dairy Science85(5), 1255-1266.
Maekawa, M., Beauchemin, K. A., & Christensen, D. A. (2002). Effect of concentrate level and feeding management on chewing activities, saliva production, and ruminal pH of lactating dairy cows. Journal of Dairy Science85(5), 1165-1175.
Martin, W. F., Armstrong, L. E., & Rodriguez, N. R. (2005). Dietary protein intake and renal function. Nutrition & Metabolism2, 1-9.
McDonald, P., Edwards, R. A., Greenhalgh, J. F. D., Morgan, C. A., Sinclair, L. A., & Wilkinson, R. G. (2002). Animal nutrition 6th edition. London and New York: Longman.
Moya, D., Holtshausen, L., Marti, S., Gibb, D. G., McAllister, T. A., Beauchemin, K. A., & Schwartzkopf-Genswein, K. (2014). Feeding behavior and ruminal pH of corn silage, barley grain, and corn dried distillers' grain offered in a total mixed ration or in a free-choice diet to beef cattle. Journal of Animal Science92(8), 3526-3536.
Mohammad, M. E. A., Gorgulu, M., & Goncu, S. (2017). The effects of total mixed ration and separate feeding on lactational performance of dairy cows. Asian Research Journal of Agriculture5(2), 1-7.
Nikkhah, A. (2013). Feeding frequency interfacing tradition and modernity in dairy production: feeding behavior insights. Journal of Animal and Poultry Sciences2(4), 91-97.
National Research Council (2007). Committee on Nutrient Requirements of Small Ruminants. Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids.
Owens, F. N., Secrist, D. S., Hill, W. J., & Gill, D. R. (1998). Acidosis in cattle: a review. Journal of Animal Science76(1), 275-286.
Rashid, M. A., Khan, M. J., Khandoker, M. A. M. Y., & Monir, M. M. (2016). Feeding different forms of ration including compound pellet and performance of growing black bengal goat. IOSR Journal of Agricultural and Veterinary Science, 9(5), 1-8.
Samanta, A. K., Singh, K. K., Das, M. M., Maity, S. B., & Kundu, S. S. (2003). Effect of complete feed block on nutrient utilisation and rumen fermentation in Barbari goats. Small Ruminant Research48(2), 95-102.
Sofi Siavash, R., & Jan Mohammadi, H. (2009). Animal nutrition. 14th Ed. Amidi pub, Amidi, Tabriz. P. 908. [In Persian]
Solanas, E., Castrillo, C., Serrano, X., Janacua, H., Fondevila, M., & Guada, J. A. (2005). Effect of concentrate extrusion and castration on diet digestion and performance of intensively reared male calves. Livestock Production Science94(3), 225-236.
Stone, W. C. (2004). Nutritional approaches to minimize subacute ruminal acidosis and laminitis in dairy cattle. Journal of Dairy Science87, E13-E26.
Strusińska, D., Minakowski, D., Bomba, G., Otrocka-Domagała, I., Wiśniewska, M., & Tywończuk, J. (2009). Effect of whole cereal grains contained in the ration on calf performance and selected morphometric parameters of the rumen and small intestine. Czech Journal of Animal Science54(12), 540-551.
Tabatabaei, S. M. M. (2003). Aspects physiological of animal nutrition. Bu-Ali Sina University Publications, P. 758. [In Persian]
Terefe, G., Kitaw, G., Walelegne, M., Fekadu, D., Kehaliew, A., Dejene, M., ... & Yadesssa, E. (2021). Growth performance of jersey calves fed maize stover silage based total mixed ration compared to calves fed hay and concentrate separately. Ethiopian Journal of Agricultural Sciences31(1), 93-100.
Theurer, C. B. (1986). Grain processing effects on starch utilization by ruminants. Journal of Animal Science63(5), 1649-1662.
Theurer, C. B., Huber, J. T., Delgado-Elorduy, A., & Wanderley, R. (1999). Invited review: summary of steam-flaking corn or sorghum grain for lactating dairy cows. Journal of Dairy Science82(9), 1950-1959.
Van Soest, P. J. (1994). Nutritional Ecology of the Ruminant. Cornell University Press, USA.
Yang, W. Z., Beauchemin, K. A., & Rode, L. M. (2001). Barley processing, forage: concentrate, and forage length effects on chewing and digesta passage in lactating cows. Journal of Dairy Science84(12), 2709-2720.
Yavuz, E., Todorov, N. A., Ganchev, G., & Nedelkov, K. (2015). Effect of physical form of starter feed on intake, growth rate, behavior and health status of female dairy calves. Bulgarian Journal of Agricultural Science21(4), 893-900.
Zhang, C., Li, M. M., Al-Marashdeh, O., Gan, L. P., Zhang, C. Y., & Zhang, G. G. (2019). Performance, rumen fermentation, and gastrointestinal microflora of lambs fed pelleted or unpelleted total mixed ration. Animal Feed Science and Technology253, 22-31.
Zhong, R. Z., Fang, Y., Zhou, D. W., Sun, X. Z., Zhou, C. S., & He, Y. Q. (2018). Pelleted total mixed ration improves growth performance of fattening lambs. Animal Feed Science and Technology242, 127-134.