اثر مکمل جیره‌ای پودر ژل آلوئه‌ورا بر عملکرد، وزن اندام‌های لنفوئیدی و پاسخ ایمنی جوجه بلدرچین‌های ژاپنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

2 استادیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

3 دانشیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان

چکیده

این آزمایش به منظور ارزیابی اثر مکمل جیره‌ای پودر ژل آلوئه‌ورا روی عملکرد و پاسخ ایمنی هومورال جوجه بلدرچین‌های ژاپنی انجام شد. هفتصد قطعه جوجه بلدرچین یک روزه در یک طرح کاملاً تصادفی با 4 تیمار، هر تیمار با 5 تکرار (35 پرنده برای هر تکرار) به واحد های آزمایشی اختصاص یافتند. تیمارهای آزمایشی شامل 1) گروه شاهد (جیره پایه، بدون پودر ژل آلوئه‌ورا) و 2)، 3) و 4) به ترتیب جیره پایه مکمل شده با 1/0، 2/0 و 3/0 درصد پودر ژل آلوئه‌ورا بودند. نتایج نشان داد که خوراک مصرفی، وزن و ضریب تبدیل غذایی پایان دوره جوجه بلدرچین‌ها تحت تاثیر سطوح مختلف پودر ژل آلوئه‌ورا قرار نگرفت (05/0<P). مصرف سطوح مختلف پودر ژل آلوئه‌ورا سبب بهبود وزن اندام‌های لنفوئیدی شد که این اختلاف برای وزن نسبی تیموس در سطح 3/0 درصد پودر ژل آلوئه‌ورا نسبت به تیمار شاهد معنی‌دار بود (05/0P<). اگرچه اثرات مصرف پودر ژل آلوئهورا روی اوزان نسبی بورس فابریسیوس و طحال و همچنین عیار  IgTو  IgGمعنی‌دار نبودند (05/0<P)، ولی روند تغییرات مشاهده شده با افزایش سطح مصرف پودر ژل آلوئه‌ورا افزایشی بود. مصرف پودر ژل آلوئه‌ورا سبب بهبود در تولید IgM علیه سلول‌های خونی قرمز گوسفند (SRBC شد به طوریکه تیمار 3/0 درصد پودر ژل آلوئه‌ورا تفاوت معنی‌داری با تیمار شاهد داشت (05/0P<). بنابراین و با توجه به موثر بودن پودر ژل آلوئه‌ورا در ارتقای سیستم ایمنی همورال و وزن اندام‌های لنفوئیدی، امکان موثر بودن این ترکیب فایتوبیوتیکی در شرایط استرسی خاص از جمله بیماری پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of dietary Aloe vera gel powder supplementation on performance, lymphoid organ weights and immune response of Japanese quails

نویسندگان [English]

  • A. Jafarzadeh 1
  • H. Darmani Kuhi 2
  • N. Ghavi Hossein-Zadeh 2
  • M. Roostaei-Ali Mehr 3
1 Graduated MSc student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Iran
2 Assistant professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Iran
3 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Iran
چکیده [English]

This experiment was conducted in order to evaluate the effects of dietary Aloe vera gel powder on performance, and humoral immunity of Japanese quails. Seven hundred one day old Japanese quails were allocated in a completely randomized design with 4 treatments replicated 5 times each with 35 birds per replicate. The experimental treatments were: 1) control group (basal diet, diet without aloe vera gel powder), 2) basal diet + 0.1 percent of aloe vera gel powder.3) basal diet + 0.2 percent of aloe vera gel powder, and 4) basal diet + 0.3 percent of aloe vera gel powder. The results did not show any significant differences between dietary treatments for feed intake, feed conversion ratio and weight gain (P>0.05). Statistically, a higher thymus weight (% of body weight) was found for group with 0.3% Aloe vera gel powder compared to the control group (P<0.05). The relative weights of spleen and bursa of fabricius and total immunoglobulin (Ig) and IgG antibody titers against SRBC were increased by increasing the levels of Aloe vera gel powder in the diets but these differences were not significant (P>0.05). The highest IgM antibody against Sheep Red Blood Cell (SRBC) was obtained by 0.3% Aloe vera gel powder group (P<0.05). In spite of non-effective impact of Aloe vera gel powder on the performance of the birds, the improved response of immune system found with this study could be beneficial for the quails in some stress conditions.

کلیدواژه‌ها [English]

  • Aloe vera
  • Humoral immunity
  • Japanese quails
  • Performance

مراجع

Akhtar M., Hai A., Awais M. M., Iqbal Z., Muhammad F., Haq A. and Anwar M. I. 2011. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens. Veterinary Parasitological, 11: 59-67.
Allen C. M., Bedford M. R. and McCracken K. J. 1995. A synergistic response to enzyme and antibiotic supplementation of wheat-based diets for broilers. In: Proc. 10th Europe Symposium on Poultry Nutrition: 15-19 Oct. Antalya, Turkey, pp. 369-370.
Basmacıoglu H. H., Tokusoglu Ö. and Ergül M. 2004. The effect of oregano and rosemary essential oils or alpha-tocopheryl acetate on performance and lipid oxidation of meat enriched with n-3 PUFA’s in broilers. South African Journal of Animal Science, 34: 197-210.
Botsoglou N. A., Christaki E., Florou-Paneri P., Giannenas I., Papageorgiou G. and Spais A. B. 2004. The effect of a mixture of herbal essential oils or                         -tocopheryl acetate on performance parameters and oxidation of body lipid in broilers. South African Journal of Animal Science, 34: 52-61.
Changkang W., Hongqiang J., Jiangming T., Weiwei G., Renna S. and Qi Z. 2007. Effect of Aloe powder and extract on production performance and immune function of broiler chickens. Journal of Fujian Agriculture and Forestry University, 36: 614-617.
Chinnah A. D., Baig M., Tizard I. R. and Kemp M. C. 1992. Antigen-dependent adjuvant activity of a polydispersed B-(1,4)-linked acetylated mannan. Vaccine, 10: 551–558.
Corrier D. E. and DeLoach J. R. 1990. Evaluation of cell-mediated cutaneous basophil hypersensitivity in young chickens by an interdigital skin test.Poultry Science, 69: 403- 408.
Cyster J. G. 2005.Chemokines, sphingosine-1-phosphate, and cell migration in secondary lymphoid organs. Annual Review of Immunology, 23: 127- 159.
Darabighane B., Zarei A., ZareShahneh A. and Mahdavi A. 2011. Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broiler. Italian Journal of Animal Science, 10: 189-194.
Darabighane B., Zarei A. and ZareShahneh A. 2012. The effects of different levels of Aloe vera gel on ileum microflora population and immune response in broilers: a comparison to antibiotic effects. Journal of Applied Animal Research, 40: 31-36.
Durrani F., Sanaullah R., Chand N., Durrani Z. and Akhtar S. 2008. Using aqueous extract of Aloe gel as anticoccidial and immunostimulant agent in broiler production. Sarhad Journal Agriculture, 24: 665-669.
Grasman K. A. 2010. Immunotoxicity testing. Methods and protocols. Department of Microbiology and Immunology. College of Veterinary Medicine, Cornell UniversityIthaca, NY 14853, USA. Pp, 387-398.
Hernández F., Madrid J., Orengo J., and Megias M. D. 2004. Influence of two plant extracts on broilers performance, digestibility and digestive organ size. Poultry Science, 83: 169-174.
Im S. A., Oh S. T., Song S., Kim M. R., Kim D. S., Woo S. S., Jo T. H., Park Y. I. and Lee C. K. 2005. Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunemodulatory activity. International Immunopharmacology, 5: 271-279.
Jagadeeswaran A. 2012. Effect of supplementation of Aloe vera extracts on growth performance in commercial broilers. Available at: http://www.notoare.com.
Jyotsana M., Arun S., Nazma I. and Harwinder S. 2008. Immunomodulatory properties of Aloe vera gel in mice. International Journal of Green Pharmacy, 2: 152-154.
Karaca K., Sharma J. M. and Nordgren R. 1995. Nitric oxide production by chicken macrophages activated by acemannan, a complex carbohydrate extracted from Aloe vera. International Journal of Immunopharmacology, 17: 183-188.
Kathi J., Kemper M. D. and Victoria B. 2013. Longwood herbal task force. Available at: http://www.mcp.edu/herbal/default.htm.
Koreleski J. and Swiatkiewicz S. 2007. Effect of coneflower, thyme and sage extracts in the diet on changes in chicken white meat quality during storage. Polish Journal of Food and Nutrition Science, 57: 303-307.
Lavinia S., Dumitrescu G., Drinceanu D., Stef D., Mot D., Julean C., Tetileanu R. and Corcionivoschi N. 2009. The effect of medicinal plants and plant extracted oils on broiler duodenum morphology and immunological profile. Romanian Biotechnological Letters, 14: 4606-4614.
Lee Y.C. 1988. Mannose-binding proteins of animal origin. Advances in Experimental Medicine and Biology, 228: 105-121.
Li P. and Zhao C. 2010. Antibacterial test of aqueous extract and ethanol extract of floslonicerae Japonica. China Modern Medicine, 17: 48-48.
Lin J., Zhang F. Y., Xu Y., Ting Z. X. and Po Y. D. 2005. Effects of gel, polysaccharide and acemannan from Aloe vera on broiler gut flora, microvilli density, immune function and growth performance. Chinese Journal of Veterinary Science, 25: 668-671.
Mehala C. and Moorthy M. 2008a. Production performance of broilers fed with Aloe vera and Curcuma longa (Turmeric). International Journal of Poultry Science, 7: 852-856.
Mehala C. and Moorthy M. 2008b. Effect of Aloe vera and Curcuma longa (Turmeric) on carcass characteristics and biochemical parameters of broilers. International Journal of Poultry Science, 7: 857-861.
Mmereole F. U. C. 2011. Evaluation of the dietary inclusion of Aloe vera as an alternative to antibiotic growth promoter in broiler production. Pakistan Journal of Nutrition, 10: 1-5.
Moorthy M., Mehala C., Saravanan S. and Edwin S. C. 2009. Aloe vera in white Leghorn layer diet. International Journal of Poultry Science, 8: 706-709.
Ni Y., Turner D., Yates K. M. and Tizard I. 2004. Isolation and characterization of structural components of Aloe vera L. leaf pulp. International Immunopharmacology, 4: 1745-1755.
Ni Y. and Tizard I. R. 2004. Analytical methodology: The gel-analysis of Aloe pulp and its derivatives. In Aloes: The GenusAloe; Reynolds, T., Ed.; CRC Press: Boca Raton, pp. 111-126.
Nordgren R. M., Stewart-Brown B. and Rodeberg J. H. 1992. The role of acemannan as an adjuvant for Marek’s disease vaccine. Proc. XIX. World's Poultry Congress. Amsterdam, Netherlands, 20–24 September, Pp. 165-169.
National Research Council 1994. Nutrient Requirement for Poultry. 9th Ed. National Academy Press, Washington, DC.
Ramamoorthy L, Kemp M. C, Tizard I. R. 1996. Acemannan, a beta-(1,4)-acetylated mannan, induces nitric oxide production in macrophage cell line RAW 264.7. Molecular Pharmacology, 50: 878-884.
Reynolds T. and Dweck A. C. 1999. Aloe vera leaf gel: a review update. Journal of Ethnopharmacology, 68: 3-37.
Schrank C. S., Cook M. E. and. Hansen W. R. 1990. Immune response of mallard ducks treated with immuno suppressive agents: antibody response to erythrocytes and in vivo response to phytohemagglutinin-p. Journal of Wildlife Diseases, 26: 307-315.
Sinurat A. P., Purwadaria T., Togatorop M. H., Pasaribu T., Bintang I. A. K., Sitompul S. and Rosida J. 2002. Responses of broilers to Aloe vera bioactives as feed additive: the effect of different forms and levels of bioactives on performances of broilers. Journal of ILmuTernak danVeteriner, 7: 69-75.
SPSS 2011. SigmaPlot 5.0 User’s Guide. Chicago: SPSS Inc.
Talmadge J., Chavez J., Jacobs L., Munger C., Chinnah T., Chow J. T, Williamson D. and Yates K. 2004. Fractionation of Aloe vera inner gel, purification and molecular profiling of activity. International Immunopharmacology, 4: 1757-1773.
Tietze C., Schlesinger P. and Stahl P. 1982. Mannose-specific endocytosis receptor of alveolar macrophages: demonstration of two functionally distinct intracellular pools of receptor and their roles in receptor recycling. The Journal of Cell Biology, 92: 417-424.
Vahedi V., Taghavi M., Maleki A. K. and Habibian R. 2011. The effect of Aloe vera extract on humoral and cellular immune response in rabbit. African Journal of Biotechnology, 10: 5225-5228.
Vazquez B., Avila G., Segura D., and Escalante B. 1996. Anti-inflammatory activity of extracts from Aloe vera gel. Journal of Ethnopharmacology, 55: 69-75.
Womble D. and Helderman J. H. 1988. Enhancement of allo responsiveness of human lymphocytes by Acemannan (Carrisyn TM). International Journal of Immunopharmacology, 10: 967-974.
Yim D., Kang S. S., Kim D. W., Kim S. H., Lillehoj H. S. and Min W. 2011. Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens. Experimental Parasitological, 127: 322–325.
Zhang L. and Tizard I. R. 1996. Activation of a mouse macrophage line by acemannan: the major carbohydrate fraction from Aloe vera gel. Immunopharmacology, 35: 119-128.

فایل ها

هم رسانی

ارجاع به این مقاله

آمار