اثر افزودن زرده تخم مرغ به رقیق کننده شیر پس چرخ و شوک سرما بر ذخیره سازی اسپرم پوشش دارشده قوچ تالشی به صورت مایع در سرما

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی دام دانشکده علوم کشاورزی دانشگاه گیلان

2 دانشیار گروه علوم دامی دانشکده علوم کشاورزی دانشگاه گیلان

چکیده

این آزمایش به منظور تعیین اثر زرده تخم مرغ و شوک سرما بر اسپرم پوشش­دارشده، با استفاده از آزمون فاکتوریل 2×5 با 10 تیمار و 5 تکرار در قالب طرح کاملاً تصادفی به­صورت اندازه­گیری­های تکرارشده در زمان انجام شد. منی از سه راس قوچ در پنچ نوبت با استفاده از واژن مصنوعی متصل به لوله حاوی تریس- فروکتوز- 15% زرده تخم­مرغ  جمع­آوری شد. نمونه­ها تجمیع، سانتریفیوژ و مایع رویی حذف شد. رسوب به دو گروه و هر گروه به پنج زیر گروه مساوی تقسیم شد و بعد به هر قسمت صفر، 5، 10، 15 و 20% زرده تخم­مرغ اضافه شد. نمونه های گروه اول به سرعت (شوک سرما) و نمونه­های گروه دوم به­تدریج تا 5 سانتیگراد سرد شدند و بعد برای 72 ساعت ذخیره شدند. تحرک پیش­رونده اسپرم، سلامت غشا پلاسمایی، زنده­مانی (هوخست بیس بنزامید33258) و واکنش آکروزومی (آلکسافلور-488) در ساعت صفر، 24، 48 و 72 بررسی شد. زنده­مانی اسپرم طی شوک سرما و سردکردن تدریجی در غلظت 5% زرده تخم مرغ (به ترتیب 47/1±60/67، 47/1±30/77) نسبت به صفر درصد (به ترتیب 47/1±750/59، 47/1±20/71) بیشتر بود. تحرک پیش­رونده اسپرم، سلامت غشا پلاسمایی و آکروزومی اسپرم در حضور زرده تخم مرغ بهبود یافت، اما در تیمارهای حاوی زرده تخم­مرغ تفاوت نداشت. بنابراین جهت ذخیره­سازی اسپرم پوشش­دارشده قوچ در رقیق­کننده شیر پس­چرخ و دما °C5 استفاده از 5% زرده تخم مرغ پیشنهاد می­شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of the addition of egg yolk to skim milk extender and cold shock on ram coated spermatozoa under cold liquid storage

نویسندگان [English]

  • A. Mohamadei 1
  • M. Roostaei-Ali Mehr 2
1 Graduate M.Sc. Student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

This experiment was conducted to determine the effect of egg yolk levels and cold shock on coated spermatozoa by using 2 × 5 factorial arrangement of the ten treatments and 5 replicates under a repeated measure randomize design. At 5 sessions, semen was collected from three ram by artificial vagina contact with a tube containing Tris- fructose-egg yolk 15%. Samples were pooled, centrifuged and removed supernatant. Aliquot was split into two groups and each one was split into 5 subgroups and after that it was added egg yolk 0, 5, 10, 15 and 20%. First group was encountered with cold shock and second group was gradually cooled up to 5◦C then samples were incubated for 72 h. Progressive sperm motility, plasma membrane integrity, viability (by Hoechst 33258 fluorescent staining) and acrosome reaction (by PNA-Alexa flur-488) were investigated at 0, 24, 48 and 72 h. Sperm viability under cold shock and gradual cooling was higher in 5% egg yolk (67.6 ±1.47and 77.3±1.47, respectively) than 0% egg yolk (59.75±1.47 and 71.2±1.47, respectively). Sperm progressive motility, plasma membrane and acrosome integrity were improved in present of egg yolk; but, there was no difference among the treatments containing egg yolk. It was suggested that 5% egg yolk was superior to keep the function of ram coated spermatozoa for storage at 5ºC in skim milk extender.

کلیدواژه‌ها [English]

  • Skim milk
  • Egg yolk
  • cold shock
  • Coating
  • Taleshi ram

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