اثر افزودن زرده تخم مرغ به رقیق کننده شیر پس چرخ و شوک سرما بر ذخیره سازی اسپرم پوشش دارشده قوچ تالشی به صورت مایع در سرما

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی دام دانشکده علوم کشاورزی دانشگاه گیلان

2 دانشیار گروه علوم دامی دانشکده علوم کشاورزی دانشگاه گیلان

چکیده

این آزمایش به منظور تعیین اثر زرده تخم مرغ و شوک سرما بر اسپرم پوشش­دارشده، با استفاده از آزمون فاکتوریل 2×5 با 10 تیمار و 5 تکرار در قالب طرح کاملاً تصادفی به­صورت اندازه­گیری­های تکرارشده در زمان انجام شد. منی از سه راس قوچ در پنچ نوبت با استفاده از واژن مصنوعی متصل به لوله حاوی تریس- فروکتوز- 15% زرده تخم­مرغ  جمع­آوری شد. نمونه­ها تجمیع، سانتریفیوژ و مایع رویی حذف شد. رسوب به دو گروه و هر گروه به پنج زیر گروه مساوی تقسیم شد و بعد به هر قسمت صفر، 5، 10، 15 و 20% زرده تخم­مرغ اضافه شد. نمونه های گروه اول به سرعت (شوک سرما) و نمونه­های گروه دوم به­تدریج تا 5 سانتیگراد سرد شدند و بعد برای 72 ساعت ذخیره شدند. تحرک پیش­رونده اسپرم، سلامت غشا پلاسمایی، زنده­مانی (هوخست بیس بنزامید33258) و واکنش آکروزومی (آلکسافلور-488) در ساعت صفر، 24، 48 و 72 بررسی شد. زنده­مانی اسپرم طی شوک سرما و سردکردن تدریجی در غلظت 5% زرده تخم مرغ (به ترتیب 47/1±60/67، 47/1±30/77) نسبت به صفر درصد (به ترتیب 47/1±750/59، 47/1±20/71) بیشتر بود. تحرک پیش­رونده اسپرم، سلامت غشا پلاسمایی و آکروزومی اسپرم در حضور زرده تخم مرغ بهبود یافت، اما در تیمارهای حاوی زرده تخم­مرغ تفاوت نداشت. بنابراین جهت ذخیره­سازی اسپرم پوشش­دارشده قوچ در رقیق­کننده شیر پس­چرخ و دما °C5 استفاده از 5% زرده تخم مرغ پیشنهاد می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of the addition of egg yolk to skim milk extender and cold shock on ram coated spermatozoa under cold liquid storage

نویسندگان [English]

  • A. Mohamadei 1
  • M. Roostaei-Ali Mehr 2
1 Graduate M.Sc. Student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

This experiment was conducted to determine the effect of egg yolk levels and cold shock on coated spermatozoa by using 2 × 5 factorial arrangement of the ten treatments and 5 replicates under a repeated measure randomize design. At 5 sessions, semen was collected from three ram by artificial vagina contact with a tube containing Tris- fructose-egg yolk 15%. Samples were pooled, centrifuged and removed supernatant. Aliquot was split into two groups and each one was split into 5 subgroups and after that it was added egg yolk 0, 5, 10, 15 and 20%. First group was encountered with cold shock and second group was gradually cooled up to 5C then samples were incubated for 72 h. Progressive sperm motility, plasma membrane integrity, viability (by Hoechst 33258 fluorescent staining) and acrosome reaction (by PNA-Alexa flur-488) were investigated at 0, 24, 48 and 72 h. Sperm viability under cold shock and gradual cooling was higher in 5% egg yolk (67.6 ±1.47and 77.3±1.47, respectively) than 0% egg yolk (59.75±1.47 and 71.2±1.47, respectively). Sperm progressive motility, plasma membrane and acrosome integrity were improved in present of egg yolk; but, there was no difference among the treatments containing egg yolk. It was suggested that 5% egg yolk was superior to keep the function of ram coated spermatozoa for storage at 5ºC in skim milk extender.

کلیدواژه‌ها [English]

  • Skim milk
  • Egg yolk
  • cold shock
  • Coating
  • Taleshi ram
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