تاثیر وزن هفت روزگی بلدرچین سویه مانچوریا بر میزان بیان ژن IGF-I، وزن زنده و وزن لاشه در سن سی و پنج روزگی

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار، گروه علوم کشاورزی، دانشگاه فنی و حرفه‌ای، کرمان، ایران

چکیده

در این تحقیق برای بررسی ارتباط بین وزن هفت روزگی و بیان ژن IGF-I در 35 روزگی در عضلات ران و سینه از 300 قطعه بلدرچین سویه مانچوریا در قالب طرح کاملاً تصادفی استفاده شد. در روز هفتم پرورش، جوجه بلدرچین‌‌ها وزن شده و به سه گروه سبک، متوسط و سنگین گروه‌بندی شدند. میزان بیان ژن IGF-I در روز 35 دوره پرورش در عضلات ران و سینه این سویه بلدرچین به روش Real-time PCR مورد بررسی قرار گرفت. همچنین در این سن، وزن سینه، ران، وزن زنده و لاشه بلدرچین­ها اندازه‌گیری شد. بیان ژن IGF-I در گروه سبک وزن کمترین و گروه سنگین وزن بیشترین میزان را به خود اختصاص داد و در بلدرچین‌‌های با وزن متوسط، میزان بیان این ژن در وسط دو گروه دیگر قرار داشت (05/0>P). علاوه بر این، تمام صفات در گروه سنگین وزن، سطح بالاتری نسبت به گروه سبک وزن داشتند (05/0>P). با توجه به این تحقیق می‌توان چنین نتیجه‌گیری کرد که پایین بودن وزن هفت روزگی سبب کاهش بیان ژن IGF-I و وزن 35 روزگی در بلدرچین سویه مانچوریا خواهد شد. با توجه به نقش ژن IGF-I در رشد و نمو، افزایش بیان این ژن در این سویه از بلدرچین می تواند سبب افزایش میزان رشد شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Influence of seven days old weight of Manchuria strain quail on IGF-I gene expression, live weight at the age of 35 days old, and carcass weight

نویسنده [English]

  • V. Bahrampour
Assistant Professor, Department of Agricultural Science, Technical and Vocational University (TVU), Kerman, Iran
چکیده [English]

In this study, the relationship between the seven days old weight and the IGF-I gene expression in the breast and thigh muscles of 300 Manchuria quail was studied in a completely randomized design at 35 days of age. On the seventh day, quails were divided into three groups of light, medium, and heavy weight. The amount of IGF-I gene expression in the thigh and breast muscles of quails was evaluated using the Real-time PCR on day 35. At this age, the weight of the breasts, thighs, and live weight were measured in quails. The IGF-I gene expression was the lowest in the light weight group and the highest was in the heavy weight group. In the medium weight quails, the amount of gene expression was between the amounts in the other two groups (P<0.05). Also, all traits were higher in the heavy group than those in the light weight group (P<0.05). According to the results of this study, it can be concluded that low weight in the seven days of age will reduce IGF-I gene expression and weight at 35 days of age in the Manchuria quail. Increasing the expression of this gene in the present strain of quail can increase the growth rate. Reducing the IGF-I gene expression in the Manchuria quail reduced body weight and weight gain. Because this gene causes the rate of growth and the amount of expression of this gene was low in quail with low weight and low growth rate. Because of the role of the IGF-I gene in growth and development, an increase of the IGF-I gene expression can cause an increase in the growth in this quail strain.

کلیدواژه‌ها [English]

  • Manchuria quail
  • IGF-I gene expression
  • Thigh muscle
  • Breast muscle
  • Real-time PCR
ایرانمنش م.، اسمعیلی زاده کشکوئیه ع.، محمدآبادی م. ر، و سهرابی س. 1395. شناسایی جایگاههای ژنی موثر بر سرعت رشد و نسبت کیلبر روی کرموزم شماره پنج بلدرچین ژاپنی. تحقیقات تولیدات دامی، 4: 12-22.
رستم زاده آ.، اسدی فوزی م.، اسدی م.، و اسماعیلی زاده ع. 1394. بررسی اثر وزن اولیه بر بیان ژن IGF-I در عضله سینه بلدرچین ژاپنی. پژوهش‌های تولیدات دامی، 8: 19-26.
Balthazart J., Baillien M., Charlier T. D., Cornil C. A. and Ball G. F. 2003. The neuroendocrinology of reproductive behavior in Japanese quail. Domestic Animal Endocrinology, 25: 69-82.
Beccavin C., Chevalier B., Cogburn L. A., Simon J. and Duclos M. J. 2001. Insulin-like growth factors and body growth in chickens divergently selected for high or low growth rate. Journal of Endocrinology, 168: 297-306.
Bomgaardt J. and Baker D. H. 1973. Effect of age on the lysine and sulfur amino acid requirement of growing chickens, Poultry Science, 52: 592-597.
Bottje W. G. and Carstens G. E. 2008. Association of mitochondrial function and feed efficiency in poultry and livestock species. Journal of Animal Science, 87: E48-E63.
Butler A. A. and LeRoith D. 2010. Minire view: tissue-specific versus generalized gene targeting of the igf1 and igf1rgenes and their roles in insulin-like growth factor physiology. Journal of Endocrinology, 142: 1685-1688.
Cain J. R. and Cawley W. O. 1972. Care management propagation. Japanese quail (coturnix). Texas Agricultural Experiment Station. Retrieved June 23, 1972. from https: //agriliferesearch.tamu.edu.
Duclos M. J. 2005. Insulin-like growth factor-I (IGF-I) mRNA levels and chicken muscle growth. Journal of Physiological Pharmacology, 3: 25-37.
Edgar R. C. 2004. Muscle: multiple with high accuracy and high throughput. Gene runner 4.0.9.68 beta. Nucleic Acids Research, 32: 1792-1797.
Genchev G. S., Mihaylova A., Ribarski M. and Kabakchie V. 2008. Meat quality and composition in Japanese quails. Trakia Journal of Sciences, 6: 72-82.
Guernec A., Berri C., Chevalier B., Wacrenier-Cere N., Le E. and Duclos M. J. 2003. Muscle development, insulin-like growth Factor-I and myostatinm RNA levels in chickens selected for increased breast muscle yield. Growth Hormone and IGF Research, 13: 8-18.
Lei M. M., Nie Q. H., Peng X., Zhang D. X. and Zhang Q. 2005. Single nucleotide polymorphisms of the chicken insulin-like factor binding protein2 gene associated with chicken growth and carcass traits. Poultry Science, 84: 1191-1198.
Mills A. D. and Faure J. M. 1991. Divergent selection for duration of tonic immobility and social reinstatement behavior in Japanese quail (Coturnix coturnix japonica) chicks. Journal of Comparative Psychology, 105: 25-38.
Mohammadabadi M. R., Nikbakhti M., Mirzaee H. R., Shandi A., Saghi D. A., Romanov M. N., Moiseyeva I. G. 2010. Genetic variability in three native Iranian chicken populations of the Khorasan province based on microsatellite markers. Russian Journal of Genetics, 46: 505-509.
Moradian H., Esmailizadeh A. K., Sohrabi S., Nasirifar E., Mohammadabadi M. R., Baghizadeh A. 2014. Genetic analysis of an F2 intercross between two strains of Japanese quail provided evidence for quantitative trait loci affecting carcass composition and internal organs. Molecular Biology Reports, 41: 4455-4462.
National Research Council. 1994. Nutrient Requirements of Poultry, 9th edition National Academy Press. Washington. D.C.
Ori R. J., Esmailizadeh A. K., Charati H., Mohammadabadi M. R. and Sohrabi S. S. 2014. Identification of QTL for live weight and growth rate using DNA markers on chromosome 3 in an F2 population of Japanese quail. Molecular Biology Reports, 41: 1049-1057.
Obolewska A., Elminowska-Wenda G., Bogucka J., Szpinda M., Walasik K. and Bednarczyk M. 2011. Myogenesispossibilities of its stimulation in chickens. Folia Biology (Krakow), 59: 85-90.
Oguzet I., Altan O., Kirkpinar F. and Settar P. 1996. Body weights, carcass characteristics, organ weights, abdominal fat, and lipid content of liver and carcass in two lines of Japanese quail (Coturnix coturnix japonica), unselected and selected for four-week body weight. British Poultry Science, 37: 579-588.
Parvin R., Mandal A. B., Singh S. M. and Thakur R. 2010. Effect of dietary level of methionine on growth performance and immune response in Japanese quails (Coturnix coturnix japonica). Journal of the Science of Food and Agriculture, 90: 471-481.
Pfaffl M. W., Horgan G. W. and Dempfle L. 2002. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30(9): e36.
Ratnamohan N. 1985. The management of Japanese quail and their use in virological research. A review. Veterinary Research Communications, 9: 1-14
SAS Institute. 1999. SAS/STAT Users Guide. SAS Inc, NC.
Sjogren K., Liu J. L., Blad K., Skrtic S., Vidal O. and Wallenius V. 1999. Liver derived insulin-like growth factor I (IGF-I) is the principal source of IGF-I in blood but is not required for postnatal body growth in mice. Proceedings of the National Academy of Science of the USA, 96: 70-92.
Sohrabi S. S., Esmailizadeh A. K., Baghizadeh A., Moradian H., Mohammadabadi M. R., Askari N. and Nasirifar E. 2012. Quantitative trait loci underlying hatching weight and growth traits in an F2 intercross between two strains of Japanese quail. Animal Production Science, 52: 1012-1018.
Velloso C. P. 2008. Regulation of muscle mass by growth hormone and IGF-I. British Journal of Pharmacology, 154: 557-568.
Yakar S., Liu J. L., Stannard B., Butler D. and Sauer B. 1999. Normal growth and development in the absence of hepatic insulin-growth factor I. Proceedings of the National Academy of Science of the USA, 96: 7324-7329.
Zhou H., Mitchell A. D., McMurtry J. P., Ashwell C. M. and Lamont S. J. 2005. Insulin-like growth Factor-I gene polymorphism associations with growth, body composition, skeleton integrity and metabolic traits in chickens. Poultry Science, 84: 212-221.