آثار مهارکننده‌های آروماتاز بر عملکرد و برخی فراسنجه‌های تولیدمثلی در بلدرچین نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد فیزیولوژی دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه کردستان

2 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه کردستان

3 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه کردستان

چکیده

آنزیم آروماتاز در تعدادی از بافت‌ها بیان شده و آندروژن‌ها را به استروژن‌ها تبدیل می‌کند. آثار زیستی مهار این آنزیم در بعضی از مهره‌داران بررسی شده است. این مطالعه به منظور بررسی آثار یک داروی مهارکننده آروماتاز (لتروزول) و یک ترکیب گیاهی دارای مهارکننده طبیعی آروماتاز (آفرودیت) بر فراسنجه‌های خونی، سرمی، تولیدمثلی و هیستوپاتولوژی بیضه و اپیدیدیم در بلدرچین نر انجام شد. بدین منظور، تعداد 120 قطعه بلدرچین نر از سن 21 روزگی در قالب یک طرح کاملاً تصادفی به سه تیمار، پنج تکرار و هشت پرنده در هر تکرار تقسیم شدند. تیمارهای آزمایشی شامل تیمار شاهد، تیمار دریافت­کننده 100 میکرولیتر آفرودیت و تیمار دریافت­کننده 03/0 میلی‌گرم لتروزول بودند. پرنده‌ها مواد آزمایشی را به‌صورت گاواژ دریافت نمودند. تجویز لتروزول موجب افزایش معنی‌دار وزن بدن پرندگان آزمایشی شد. همچنین افزایش معنی‌دار در افزایش روزانه وزن بدن و بهبود ضریب تبدیل غذایی در گروه‌های دریافت‌کننده لتروزول و آفرودیت مشاهده شد (05/0P<). فراسنجه‌های خونی و هورمونی تحت تأثیر گروه‌های آزمایشی قرار نگرفتند، اما مصرف آفرودیت و لتروزول موجب افزایش درصد زنده‌مانی اسپرم، سلامت آکروزوم و غشای اسپرم، و نیز کاهش تعداد اسپرم در بیضه بلدرچین‌ها شد (05/0P<). نتایج این پژوهش نشان داد استفاده از داروهای مهارکننده آروماتاز، علاوه بر اثر مطلوب بر عملکرد تولیدی، فراسنجه‌های تولیدمثلی از جمله فراسنجه‌های کیفی اسپرم شامل درصد زنده‌مانی، درصد سلامت آکروزوم و درصد سلامت غشای اسپرم بلدرچین نر را بهبود می‌بخشد. همچنین در آزمایش‌های انجام شده، آثار منفی ناشی از تجویز لتروزول مشاهده نشد و استفاده از این ترکیب می‌تواند در برنامه‌های پرورشی مد نظر قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of aromatase inhibitors on performance and some reproductive parameters in male quails

نویسندگان [English]

  • E. Shafiee 1
  • A. Vaziry 2
  • A. Farzinpour 3
1 Former MSc Student of Animal Physiology, Department of Animal Science, Faculty of Agriculture, Kurdistan University, Sanandaj, Iran
2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
3 Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
چکیده [English]

The biological effects of aromatase inhibitors in humans and some animals have been studied in several types of research. This study was performed to investigate the effects of two aromatase inhibitors: Letrozole, a synthetic aromatase inhibitor, and Aphrodite, a blend of the medicinal plant containing natural aromatase inhibitor, on blood, reproductive parameters, and also testis and epididymis morphology in male quails. The experiment was conducted with 120 male quails from the age of three weeks in a completely randomized design with three treatments, five replicates, and eight birds in each replicate. Experimental birds in the treatments received distilled water, Aphrodite (100 μL) or letrozole (0.03 mg), via oral gavage. Administration of Aphrodite and letrozole significantly increased the body weight and feed conversion ratio (P<0.05). Feed intake was significantly increased in the Aphrodite received group (P<0.05). Elevated relative weight concurrent with a lowered number of sperm in the testis was also recorded in both Aphrodit and letrozole-treated birds. Serum levels of phosphorus, albumin, total protein, estrogen, and testosterone were not affected by the experimental treatments, but consumption of Aphrodite and letrozole improved sperm viability, acrosome, and membrane integrity (P<0.05). The result showed that application of Letrozole, and Aphrodite, improved production performance and reproductive parameters, such as body weight, feed conversion ratio, sperm viability, and acrosome and membrane integrity in experimental male quails. Letrozole did not reveal any negative effects on the examined parameters and organs and could be considered as a candidate to apply in the breeding programs.The result shows that application of Letrozole and Aphrodite improved productive performance and reproductive parameters, such as body weight, feed conversion ratio, sperm viability, and acrosome and membrane integrity in experimental male quails. Letrozole did not revealed any negative effects on the examined parameters and could be considered as a candidate to apply in the birds breeding programs

کلیدواژه‌ها [English]

  • Aromatase
  • Aphrodite
  • Quail
  • Letrozole
  • Steroid hormones

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تحقیقات تولیدات دامی
دوره 10، شماره 3
آذر 1400
صفحه 67-77

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