بررسی کیفیت اسپرم پوشش دار شده قوچ بعد از 72 ساعت ذخیره سازی در 4 درجه سانتی‌گراد و افزودن مایع منی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی گروه علوم دامی دانشکده علوم کشاورزی، دانشگاه گیلان

2 دانشیار گروه علوم دامی دانشکده علوم کشاورزی، دانشگاه گیلان

چکیده

این آزمایش به منظور بررسی اثر افزودن مایع منی به اسپرم پوشش‌دار شده پس از 72 ساعت ذخیره‌سازی انجام شد. منی از 4 راس قوچ نژاد تالشی به وسیله واژن مصنوعی جمع‌آوری شد. جهت پوشش‌دارکردن اسپرم، منی در لوله حاوی تریس-فروکتوز-15 درصد زرده تخم مرغ جمع‌آوری شد. نمونه‌ها بعد از ارزیابی اولیه، تجمیع و سانتریفیوژ شدند. پس از حذف مایع ‌فوقانی، رسوب رقیق شد. نمونه به 3 بخش مساوی تقسیم و تا °C4 سرد شد و بعد به مدت 68 ساعت ذخیره شد. بخش اول نمونه‌ها سانتریفیوژ شده و مایع‌ فوقانی برداشته شد (روشI). بخش دوم نمونه‌ها سانتریفیوژ شده و دوباره با مایع‌ فوقانی مخلوط شد (روشII). بخش سوم نمونه‌ها بدون تغییر در دمای °C4 نگهداری شدند (روشIII). نمونه‌های به دست آمده از هر روش‌ به سه قسمت مساوی تقسیم شد و مقادیر صفر، 10 و 20 درصد مایع منی به آن‌ها اضافه شد و در °C4 ذخیره شدند. تحرک پیش‌رونده، سلامت غشای پلاسمایی، زنده‌مانی اسپرم و سلامت غشای آکروزوم نمونه‌ها پس از 4 ساعت بررسی شدند. زنده‌مانی در مقدار صفر درصد مایع منی (416/69%) بیشتر از مقدار 20 درصد مایع منی (667/60%) بود (05/0>P). کمترین میزان تحرک پیش‌رونده (667/11%)، زنده‌مانی اسپرم (167/59%) و سلامت غشای پلاسمایی (167/48%) در روش 1 مشاهده شد (05/0>P). روش‌ها اثری بر سلامت غشای آکروزوم نداشتند (05/0<P). بنابراین حذف زرده تخم مرغ و افزودن مایع منی به اسپرم پوشش‌دارشده پس از 72 ساعت سبب کاهش ماندگاری اسپرم می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of quality of ram coated sperm after 72 hours storage at 4"C and adding seminal plasma

نویسندگان [English]

  • A. R. Vaferi 1
  • M. Roostaei Ali-Mehr 2
1 MSc physiology student, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Associate professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

This experiment was conducted to evaluate the addition of seminal plasma to coated spermatozoa after 72 h. Semen was collected from four Taleshi rams by artificial vagina. To prepare coated spermatozoa, semen was collected in tube containing Tris-fructose-15% egg yolk. After initial evaluation, the samples were mixed and centrifuged. After removal of supernatant and dilution, samples were divided into three sections, cooled to 4°C and stored for 68 hours. The first part was centrifuged and the supernatant was removed (Method I). The second section was centrifuged and the supernatant was mixed again (Method II). The third section was kept without change at 4°C (Method III). Samples were obtained from each method were divided into three equal parts, added 0, 10 and 20% of seminal plasma and incubated at 4°C. Sperm motility, viability, plasma membrane integrity, acrosome membrane integrity were evaluated after 4 hours. Sperm viability was higher in the presence of 0% seminal plasma (69/416%) than 20% seminal plasma (60/667%; P<0/05). The lowest sperm motility (11/667%), viability (59/167%) and plasma membrane integrity (48/168%) was observed in Method I (P<0/05). Methods did not affect the integrity of the acrosome membrane (P>0.05). Therefore, removing egg yolk and adding seminal plasma reduces coated sperm quality after 72 h storage.

کلیدواژه‌ها [English]

  • Centrifuge
  • Coated Sperm
  • Seminal plasma
  • Taleshi ram

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