اثر تیمول به دو شکل آزاد و آهسته رهش بر ویژگی های تخمیر شکمبه و متابولیت های پلاسما در گوسفند

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دوره دکتری گروه علوم دامی، دانشکده کشاورزی دانشگاه بوعلی سینا

2 دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه بوعلی سینا

3 استاد گروه فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی شهید بهشتی

4 استادیار ، دانشکده دامپزشکی، دانشگاه بوعلی سینا

چکیده

این پژوهش به­منظور مقایسه اثرات تیمول به دو شکل آزاد و آهسته­رهش بر ویژگی­های تخمیر شکمبه، پروتئین میکروبی و برخی     متابولیت­های پلاسما در گوسفند انجام شد. آزمایش در قالب طرح مربع لاتین 3´3 به صورت ادغام شده با استفاده از 6 راس گوسفند نر فیستوله شده انجام شد. تیمارها عبارت بود از (الف) جیره پایه +250 میلی­گرم تیمول به شکل آزاد، (ب) جیره پایه +250 میلی­گرم تیمول به شکل آهسته­رهش و (ج) تیمار شاهد (جیره پایه بدون تیمول). هر دوره آزمایش 14 روز و شامل 10 روز سازگاری و 4 روز نمونه­گیری بود. در دوره نمونه­گیری، نمونه­های مدفوع جمع­آوری شد و در روز پایانی هر دوره نیز نمونه­های خون و مایع شکمبه در زمان­های مختلف جمع­آوری شد. مصرف تیمول به هر دو شکل آزاد و آهسته­رهش، ماده خشک مصرفی (به ترتیب 5/917 و 5/985 گرم در روز)، قابلیت هضم پروتئین خام (به ترتیب 7/82 و 6/85 درصد) و غلظت گلوکز پلاسما (به ترتیب 8/114 و 5/108 میلی­گرم در دسی­لیتر) را افزایش داد (05/0>P). در تیمارهای محتوی تیمول، غلظت اوره­ی پلاسما، سنتز پروتئین میکروبی و غلظت نیتروژن آمونیاکی در 5 و 7 ساعت پس از خوراکدهی کاهش یافت (05/0>P). تعداد دیپلودینیوم­ها در جیره محتوی تیمول آزاد 105×38/0 بود که از تعداد مربوط به دو تیمار دیگر کمتر بود (05/0>P). مصرف تیمول می­تواند با کاهش تولید نیتروژن آمونیاکی بازده استفاده از پروتئین خوراک را بهبود بخشد و با توجه به افزایش گلوکز پلاسما می­تواند اثری مثبت در تامین انرژی دام ایجاد کند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of free thymol and sustained release thymol on rumen fermentation and plasma metabolites in sheep

نویسندگان [English]

  • Z. Zamani 1
  • D. Alipour 2
  • H. R. Moghimi 3
  • S. A. Mortazavi 3
  • S. M. Zolhavarieh 4
1 PhD graduated student, Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University
3 Professor of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences
4 Assistant Professor, Department of Clinical Science, Faculty of Paraveterinary Science, Bu-Ali Sina University
چکیده [English]

This experiment was conducted to compare the effect of free and sustained release thymol on rumen fermentation characteristics, microbial protein and some plasma metabolites. Six ruminally cannulated sheep were used in a 3×3 merged Latin square design. Treatments were (a) basal diet+ 250 mg free Thymol, (b) basal diet+ 250 mg slow relrase Thymol and (c) control (basal diet without Thymol). The experiment consisted of three 14-d periods with 10 d adaptation and 4 d sampling. At sampling period, the feces were collected and at the end of each period blood sampling and rumen fluid collection were made at different times. The measured traits were: Feed digestability, N-NH3, plasma and glucose concentration, ruminal pH, microbial protein synthesis and efficiency of microbial protein synthesis. Dry matter intake (985.5 g and 917.5 g per day, respectively), crude protein digestibility (82.7% and 85.6%, respectively) and plasma glucose (114.8 mg/dL and 108 mg/dL, respectively) were increased by free and sustained release thymol consumption (P<0.05). Plasma urea concentration, microbial protein synthesis and N-NH3 were decreased in 5 and 7 hours after feeding (P<0.05). Number of diplodinia was 0.38×105 in diet with free thymol and this number was lower than those in other two treatments (P<0.05). Thymol consumption improved the efficiency of dietary protein utilization by decreasing N-NH3 and increased level of plasma glucose showed a positive effect on energy utilization of animal.

کلیدواژه‌ها [English]

  • Ammonia
  • Microbial protein
  • Thymol
  • Glucose

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